J. Planar Chromatogr. 24, 121-124 (2011). HPTLC of kurarinone and sophoraflavanone G in the roots of Sophora flavescens on silica gel with chloroform - methanol 10:1 with chamber saturation for 30 min at 20 °C. Quantitative determination by absorbance measurement at 285 nm. The intra-day and inter-day %RSD were 1.9-2.0 % and 2.2-2.4 %, respectively. Instrument precision and repeatability of the method were 0.4-0.6 and 1.81-1.84 %, respectively. Average recovery was 96.3-103.4 % for kuraninone and 96.8-102.9 % for sophoraflavanone G. The limits of detection and quantification were 27 and 80 ng/band for kurarinone and 12 and 36 ng/band for sophoraflavanone G. Linearity was in the range of 200-1200 ng/band for kurarinone and 90-550 ng/band for sophoraflavanone. The hRf value was 31 and 50 for kurarinone and sophoraflavanone, respectively.

Chinese J. of Pharm. Anal. 29 (12), 2168-2179 (2009). TLC of the extracts of 11 varieties of herbal drugs and preparations on silica gel (conditioned with 1 % iodine in dichloromethane) with cyclohexane - dichloromethane - ethyl acetate - glacial acetic acid 200:50:80:1. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C. Identification of oleanolic acid and ursolic acid by comparison of the hRf values (hRf 54 for oleanolic acid and 38 for ursolic acid, respectively) with standards.

International Journal of Pharmacy and Pharmaceutical Sciences 3(2), 85-89 (2011). TLC of andrographolide on silica gel with toluene - ethyl acetate - formic acid 10:9:1. Densitometric evaluation at 235 nm before derivatization. Evaluation of the fingerprint profile at 254 nm and after derivatization at 366 nm by spraying with anisaldehyde-sulfuric acid reagent followed by heating at 110 °C.

J. AOAC Int. 94, 795-802 (2011). HPTLC of boeravinone B and E on silica gel (prewashed with methanol) with toluene - ethyl acetate - acetonitrile - formic acid 60:12:4:3 in a twin-trough chamber after saturation for 10 min at 25 +/- 1 °C at a relative humidity of 35-40 %. Quantitative determination by absorbance measurement at 275 nm. The hRf value of boeravinone B and E was 47 and 31, respectively.The intra-day and inter-day precision (n = 3) were 0.6-1.7 % and 1.0-1.3 % for boeravinone B and 0.3-1.5 % and 1.3-1.5 % for boeravinone E. The repeatability of application and detection (%RSD) was between 0.8-1.1 % for boeravinone B and 0.3-0.9 % for boeravinone E (n=7). Linearity was between 75-360 ng/zone for boeravinone B and 160-768 ng/zone for boeravinone E. The LOD and LOQ were 9 and 13 ng/zone for boeravinone B and 30 and 42 ng/zone for boeravinone E. %RSD of robustness was <2 %. The recovery was 98.9-99.5 % for boeravinone B and 97.9-98.4 % for boeravinone E.

International Journal of PharmTech Research 2(4), 2438-2440 (2010). TLC of methanolic extracts of powdered dry fruits of Embelia ribes on silica gel with toluene - acetone - acetic acid 18:2:1. Detection with anisaldehyde-sulphuric acid reagent. The hRf values of the four major bands were 13, 27, 32, and 60.

J. Planar Chromatogr. 25, 571-574 (2012). HPTLC of theanine in tea extracts on silica gel with n-butanol - acetone - acetic acid - water 7:7:2:4. Detection by dipping into a ninhydrin reagent for 3 s, followed by heating at 105 °C for 5-15 min. Quantitative determination by analysis of green channels of photographs using the CP Atlas 2.0 software. The hRf of theanine was 35. Linearity was in the range of 1.4-14 ng/zone. The intermediate/inter-day/intra-day precision was below 0.7 % (n=3). Recovery (by standard addition) was between 95.7 and 102.5 %.

J. Planar Chromatogr. 25, 290-294 (2012). HPTLC of withaferine A (1), 1,2 deoxy-withastramonolide (2), withanolide A (3), and withanolide B (4) in Withania somnifera on silica gel with dichloromethane - toluene - methanol - acetone - diethyl ether 15:15:6:2:2. Quantitative determination by absorbance measurement at 235 nm. The hRf values of (1) to (4) were found to be 58, 61, 68 and 79, respectively. Linearity was in the range of 200-1200 ng/band. Average recoveries were 98, 99.5, 98 and 99 % for compounds (1) to (4), respectively.

J. Planar Chromatogr. 25, 433-438 (2012). HPTLC of quercetin (1) and kaempferol (2) in the leaves of Centella asiatica on silica gel with toluene - ethyl acetate - chloroform - formic acid 6:4:4:1. Quantitative determination by absorbance measurement at 240 nm. The hRf of compounds (1) and (2) were 35 and 48, respectively. Linearity was in the range of 100-1000 ng/band. Limits of detection and quantification were 54 and 165 ng/band for (1) and 68 and 207 ng/band for (2), respectively. Intra-day relative standard deviation of (1) was between 5.3 and 6.5 % and of (2) between 5.1 and 11.4 %. Inter-day relative standard deviation of (1) was 2.1-6.6 % and of (2) 2.6-5.8 % (n=6). Recovery was found to be 98.1 % for (1) and 90.1 % for (2).