59th Indian Pharmaceutical congress F-225, 443, (2007). HPTLC cinnamaldehyde in the bark powder of Cinnamomum zeylenicum on silica gel with toluene - ethyl acetate - formic acid 190:10:1. Densitometric evaluation at 295 nm for quantification. The method was linear within the range of 31 and 157 ng/zone. The identity of the compound was confirmed by over overlaying the UV spectra of sample and standard. Cinnamomum bark was found to contain 0.25 % of cinnamaldehyde. Limit of detection and quantification was 3000 and 9900 ng/mL, respectively.

Pharmazie 62, 900-901 (2007). TLC of dihydroartemisinin and the degradation products 2-(3-oxobutyl)-3-methyl-6-(2-propanol)-cyclohexanon and 2-(3-oxobutyl)-3-methyl-6-ethyl-cyclohexanon on silica gel with chloroform - methanol 19:1. Detection by spraying with vanillin reagent (0.5 g vanillin in 80 mL sulfuric acid and 20 mL ethanol).

J. AOAC Int. 91, 13-20 (2008). Comprehensive proposal for the validation of qualitative HPTLC methods. The steps of the validation process (method selection and optimization, stability, specificity, precision, and robustness) are illustrated with examples of identification methods: green tea leaf, ginseng root, eleuthero root, echinacea root, black cohosh rhizome, licorice root, kava root, milk thistle aerial parts, feverfew aerial parts, and ginger root. The validation protocol is a key element for structuring, managing and documenting the validation process. HPTLC is suitable for reliable identification of botanicals because it can provide chromatographic fingerprints that can be visualized and stored as electronic images. Reproducibility is improved if suitable instrumentation is used, a standardized HPTLC methodology is implemented, and methods have been validated.

J. AOAC Int. 91, 1149-1153 (2008). HPTLC of berberine on silica gel prewashed with methanol using n-butanol - acetic acid - water 8:1:1 in a twin-trough chamber with chamber saturation for 5 min at 33 °C at 57 % relative humidity. Quantitative determination by absorbance measurement at 350 nm.

Indian Drugs 45(8), 663-666 (2008). HPTLC of geranial and luteolin from leaves of Cymbopogon citratus on silica gel with toluene - ethyl acetate 9:1 for geranial and toluene - ethyl acetate - formic acid 10:7:1 for luteolin. Densitometric evaluation at 200 nm (geranial) and 254 nm (luteolin). Alcoholic extracts of the plant leaves were found to contain 1.34 % and 1.49 % of geranial and luteolin respectively.

J. Planar Chromatogr. 21, 263-265 (2008). HPTLC of nimbolide on silica gel in a twin trough chamber with ethyl acetate - hexane 1:1. Quantitative determination by absorbance measurement at 254 nm. The limits of detection and quantification were 3.3 and 1.0 µg/spot, respectively.

Phytochem. Anal.19, 104-115 (2008). TLC of geraniol on silica gel with chloroform - methanol - water 97:24:2 and of geranylacetate with toluene - ethyl acetate 93:7. Detection by spraying with vanillin sulfuric acid reagent.

60th Indian Pharmaceutical Congress PA-217 (2008). HPTLC of oleanolic acid in total methanolic extract of fruits of Randia dumetorum lam. on silica gel with toluene - ethyl acetate - acetic acid 70:30:1 in a twin trough chamber saturated for 10 min. Detection by treatment with 10 % sulphuric acid in methanol, followed by heating at 110 °C and immediate densitometric evaluation. Quantitative determination by absorbance measurement at 540 nm. The method was linear in the range of 50-500 ng/spot. Recovery was in the range of 99.4-100.8 %.