Chinese J. of Northwest Pharm. 28 (3), 231-235 (2013). Berberis heteropoda Schrank, a Xinjiang special medicinal herb, is a perennial shrub, produced in northwest and southwest China, and is widely used as a herbal TCM drug. It is used for the treatment of dysentery, diarrhea, swollen eyes, eczema, etc. For quality control, identification of three alkaloids by TLC of the sample extracts and the standards berberine hydrochloride, palmatine chloride and jatrorrhizine hydrochloride on silica gel with toluene – ethyl acetate – methanol – isopropanol – ammonia 12:6:4:3:1 (determined by investigating three mobile phase systems), detection under UV 366 nm. In addition to optimization of the stationary phase, the developing system and the detection technique, the method was validated by investigating the effects of the sample application volume (2-3 μL), the developing temperature (room temperature) and the relative humidity (42-65 %).

by high-performance thin-layer chromatography) (Chinese). J. Chinese Trad. Patent Med. 35 (12), 2752-2754 (2013). Chimonanthus nitens Oliv. is a plant of the Calycanthaceae family growing in Jiangxi and Anhui areas of China. Pharmacological studies showed that the chemical constituents of the extracts of its dry leaves are volatile oils, alkaloids, flavonoids, coumarins, etc. The dry leaves are used clinically as the main ingredient drug in TCM prescriptions for the treatment of infantile cough, respiratory tract infection, hand foot and mouth disease and chronic pelvic inflammatory disease, etc. For quality control, HPTLC of the sample extracts and the standards cineole, linalool and beta-caryophyllene on silica gel firstly with chloroform – methanol 9:1 to 5 cm and then with petroleum ether (30-60 °C) – ethyl acetate 10:1 to 10 cm, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 110 °C. Quantitative determination by densitometric evaluation at 606 nm for cineole and linalool and 545 nm for beta-caryophyllene using external standard calibration method. The content of the total unknown components is also given taking linalool as the calibration standard. Validation of the method by investigation of its linearity range (for cineole 0.7-11.4 µg/zone, r=0.990, n=7; for beta-caryophyllene 0.7-10.9 µg/zone, r=0.992, n=7; for linalool 1.8-18.0 µg/zone, r=0.990, n=7), precision (%RSD (n=6) 3.9 %, 1.5 % and 0.6 % for cineole, beta-caryophyllene and linalool, respectively), stability (within 30 min, %RSD (n=6) 2.0 %, 3.0 % and 3.3 % for cineole, beta-caryophyllene and linalool, respectively) and reproducibility (%RSD (n=6) 2.7 %, 4.4 % and 3.2 % for cineole, beta-caryophyllene and linalool, respectively). The recovery for cineole was 82.3-93.4 % (%RSD (n=3) 2.6-4.1 %), for beta-caryophyllene 86.1-96.7 % (%RSD (n=3) 2.2-3.6 %) and for linalool 95.8-109.8 % (%RSD (n=3) 1.9-2.9 %). Some unknown separated components need to be identified in a further study.

J. Planar Chromatogr. 27, 472-476 (2014). HPTLC of (1) genistein and (2) vitexin in seeds of Vigna mungo on silica gel with toluene – ethyl acetate – methanol – acetic acid – formic acid 6:14:2:1:1 after saturation with mobile phase for 25 min. Quantitative determination by absorbance measurement at 254 nm. The hRF values of (1) and (2) were 81 and 31, respectively. Linearities were in the range of 500-2500 ng/zone for (1) and (2). The intermediate precisions were below 8 % (n=6) for (1) and (2). The LOD and LOQ were 14 and 42 ng for (1) and 52 and 159 ng for (2), respectively. Recoveries for (1) and (2) were in the range of 96.1-97.1 and 95.7-97.6 %.

J. Jiangxi Univ. Trad. Chinese Med. 26 (2), 55-58 (2014). Xiangshao Shugan Koufuye oral liquid is a TCM preparation for the treatment of chronic gastritis, gastric ulcer, cholecystitis, etc. For quality control, TLC on silica gel (1) for Cyperus rotundus with cyclohexane – ethyl acetate – isopropanol – formic acid 70:30:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 3 min and evaluation at UV 366 nm; (2) for Paeonia lactiflora Pall. and paeoniflorin with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:4 and heating until the zones are visible; (3) for Radix Bupleuri with butanol – ethyl acetate – 10 % ammonia 4:1:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible and at UV 366 nm. Quantitation of paeoniflorin by HPLC.

J. Planar Chromatogr. 27, 346-356 (2014). HPTLC fingerprint of the leaves of Arctium lappa and Fagus sylvatica, herba of Epilobium hirsutum, Lythrum salicaria, and Artemisia dracunculoides; and fruits of Cydonia oblonga and leaves of Hippophae rhamnoides and Aronia melanocarpa on silica gel with ethyl acetate – acetic acid – formic acid – water 50:6:6:13 for flavonoid glycosides and chloroform – acetic acid – methanol – water 16:8:3:2 for saponins. Detection by immersion into natural product reagent followed by PEG 400 reagent. Qualitative identification at UV 366 nm.

J. Planar Chromatogr. 28, 167-172 (2015). TLC of trans-resveratrol in cosmetic raw materials on RP with methanol - water 3:2. The plate is dried for 3 h. Quantitative determination by fluorescence measurement using a deuterium lamp at 340 nm. The hRF value was 38. The LOD and LOQ were 2 and 6 ng/zone, respectively. The presence of trans-resveratrol in the analyzed samples was additionally confirmed by detection with anisaldehyde reagent.

J. Planar Chromatogr. 28, 294-299 (2015). HPTLC of cucurbitacin B in Lagenaria siceraria on silica gel with chloroform - methanol 19:1. Quantitative determination by absorbance measurement at 242 nm. The hRF value for cucurbitacin was 67. Linearity was in the range of 200-1200 ng/zone. LOD and LOQ were 40 and 123 ng/zone, respectively. The intermediate precision was below 6 % (n=6). Recovery ranged between 95 and 96 %.

Food Sci. Technol. Campinas. 35, 598-604 (2015). TLC of phosphatidylglycerol (1), phosphatidic acid (2), phosphatidylethanolamine (3), phosphatidylinositol (4), phosphatidylcholine (5) and lysophosphatidylcholine (6) in the seeds of flaxseed (Linum usitatissimum) on silica gel with chloroform - acetone - methanol - acetic acid - water 10:4:2:2:1. Detection by exposure to iodine vapours and evaluation in daylight. Solid phase extraction was performed to characterize the phospholipid composition during flaxseed development.