J. Planar Chromatogr. 24, 470-474 (2011). TLC of methanolic seed extracts with harmine, harmaline, and galanthamine as standards on silica gel with ethyl acetate - methanol - ammonia 20:3:1. Detection by inspection under UV 366 nm and by spraying with Dragendorff’s and vanillin-sulfuric acid reagent as well as by bioautography assay. For the bioautographic assay, AChE was dissolved in 150 mL of 0.05 M TRIS/hydrochloric acid buffer at pH 7.8. 150 mg bovine serum albumin was added for stabilization.The plates were dipped in the enzyme stock solution and incubated in a humid chamber at 37 °C for 20 min. For detection of the enzyme, solutions of 1-naphthyl acetate in ethanol and of Fast Blue B salt in water were prepared and mixed immediately before spraying the plate. The limits of AChE inhibitive activity were 10 ng/zone for all.

Chinese J. Prac. Med. 7 (3), 251-252 (2012). Presentation of a procedure for preparation and quality control of Qichai Hugan pills. TLC of the extracts 1) for Astragalus mongholicus, on silica gel with the lower phase of chloroform - ethyl acetate - methanol - water 10:20:11:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min and viewing under daylight and UV 366 nm; 2) for the root of Chinese thorowax, on silica gel with the lower phase of chloroform - methanol - water 7:3:1, detection by spraying with a solution of p-dimethylaminobenzaldehyde - sulfuric acid - ethanol 1:2:18 followed by heating at 105 °C and viewing under daylight; 3) for the fruit of Chinese wolfberry, on silica gel with toluene – ethyl acetate - formic acid 15:5:2, detection under UV 366 nm; 4) for the root of red-rooted salvia, on silica gel with chloroform - acetone - formic acid 60:5:2, detection by spraying with a solution of 2 % ferric chloride - 1 % potassium ferricyanide 1:1 and viewing under daylight.

J. of Chinese Med. 27 (166), 344-345 (2012). Biqiang Bidou flushing fluid is a herbal TCM preparation for treatment of forehead headaches and sinusitis. For quality control, TLC on silica gel 1) for Honeysuckle and the standard chlorogenic acid, with ethyl acetate – formic acid – water 15:12:5, detection under UV 366 nm; 2) for Baical Skullcap, with ethyl acetate – acetone – acetic acid – water 10:4:5:3, detection by spaying with 5 % ferric chloride in ethanol and viewing in daylight.

Modern J. of Integrated Trad. Chinese & Western Med. 21 (16), 1786-1788 (2012). Ruoshiming oral liquid is a herbal TCM preparation for the treatment of children’s optic nerve hypoplasia, myopia, hyperopia, and astigmatism. For identification, TLC of the extracts of the preparations on silica gel 1) for Radix Rehmanniae Preparata, with benzene – diethyl ether 5:2, detection by spraying with 2 % ferric chloride – 2 % potassium ferricyanide 1:1 and viewing in daylight; 2) for Pericarpium Citri Reticulatae, first with ethyl acetate – methanol – water 100:17:13 and then with the upper phase of toluene – ethyl acetate – formic acid – water 20:10:1:1, detection by spraying with 3 % aluminium chloride in ethanol and viewing at UV 366 nm; 3) for Rhizoma Acori Tatarinowii, with chloroform – diethyl ether 8:1, detection by viewing in daylight; 4) for Polygonatum sibiricum, with benzene – ethyl acetate – formic acid 50:40:1, detection by spraying with potassium iodobismuthate – HCl – water 10:1:200 and viewing in daylight; 5) for Fructus Lycii, developed with benzene – diethyl ether 2:1, detection by viewing in UV 366 nm.

Chinese J. of Trad. Chinese Med. 21 (4), 585-586 (2012). Chuanxining pills are a herbal TCM preparation effective for tonifying kidney, relieving uneasiness of mind, body tranquilization, relieving a cough,and preventing asthma. They are prescribed clinically to cure bronchitis and pneumonectasis. For quality control, identification of the three main component drugs by TLC of the extracts of the preparations 1) for Epimedium davidii Franch, on silica gel with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection by spraying with 2 % ferric chloride in ethanol and mild heating; 2) for Fructus Ligustri Lucidi, on silica gel with chloroform – methanol 40:1, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly; 3) for Angelica sinensis, on silica gel with n-hexane - ethyl acetate 9:1, detection at UV 366 nm.

Chinese J. of Trad. Chinese Med. for Emerg. 21 (5), 738-739 (2012). Ningxin pills are a TCM preparation with special efficiency on promoting blood circulation, removing blood stasis, relieving pain and are prescribed clinically to treat coronary disease and angina pectoris. For quality control, TLC of the extracts on silica gel 1) for Panax Notoginseng (Burk.) F.H.Chen, Radix Ginseng rubra, with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Rhizoma Corydalis, with toluene – acetone 9:2, detection by exposure to iodine vapors and evaluation at UV 366 nm; 3) for borneol, with petroleum ether (30-60 ºC) – ethyl acetate – chloroform 11:1:3, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight.

Chinese J. of Inform. on TCM 17 (12), 45-48 (2010). Jinshishenyan pills are a herbal TCM preparation effective for nourishing kidney, activating spleen-energy, relieving internal heat or fever, and removing blood stasis. For quality control, TLC of the extracts on silica gel for 1) Lonicera japonica, with the upper phase of ethyl acetate – formic acid – water 14:5:5, detection at UV 366 nm; 2) for Cortex Phellodendri chinensis, developed with benzene - ethyl acetate – methanol – isopropanol – concentrated ammonia 12:6:3:3:1, detection by exposure to ammonia vapors for a few minutes and evaluation at UV 366 nm.

Chinese J. of Lishizhen Trad. Med. & Pharm. 30 (11), 1499-1501 (2012). Qinyu Shaoshang burn liquid is a herbal TCM preparation for treatment of burns. For quality control TLC on silica gel plates 1) for Borneolum Syntheticum, with chloroform – methanol – ammonia 100:20:1, detection under UV 366 nm; 2) for Amur Cork-tree Bark, with petroleum ether (60-90 °C) ethyl acetate - chloroform 3:1:1, detection by spraying with 5 % phosphomolybdic acid in ethanol and viewing in daylight. Quantification of baicalin by HPLC.