Phytochemistry 30, 3781-3784 (1991). TLC of various kaem-pherol glycosides, including novel apiosides, on silica with ethyl acetate - MEK - acetic acid - water 5:3:1:1; on RP-8 with methanol - water 7:3 and HPTLC on diol with ethyl acetate - toluene 1:1. Detection under UV and by spraying with 2-aminoethylborate/polyethyleneglycol reagent. The use of diol HPTLC plates appears highly suitable for the separation of closely related flavonol glycosides. Also column chromatography and low pressure liquid chromatography.

Planta med. 70, 8-11 (2004). Preparative TLC of 6aa,12aa-12a-hydroxyelliptone, deguelin, a-toxicarol, rotenone, elliptone on silica gel using dichloromethane, benzene - methanol 47:3, n-hexane - ethyl acetate 4:1, and benzene - acetone 47:3. Detection under UV light at 254 nm.

Planta med. 68, 640-642 (2002). Analytical and preparative TLC of i. a. (+)-10,11-dioxoerysotrine, erysotrine, syringaresinol, erybraedin, neobavaisoflavone, abyssinone IV, neorautenol, isoneorautenol, shinpterocarpin, and erythrinin B on silica gel with chloroform - ethyl acetate 6:1 by 3-fold development. Visualization under UV light at 254 and 366 nm and by spraying with vanillin - sulfuric acid reagent. TLC bioautography for antibacterial and antifungal assays.

CBS 87, 6-7 (2001). HPTLC on silica gel with chloroform - methanol - water 6:4:1. Detection by spraying with 10 % ethanolic sulfuric acid solution, followed by heating at 120 °C. Quantitative determination by absorbance measurement at 450 nm.

J. Planar Chromatogr. 17, 14-17 (2004). HPTLC of oxindole alkaloids on silica gel, Diol-, CN-, NH2-, and RP-18-phases. Normal-phase TLC with 1) ethyl acetate - methanol - water 200:27:20; 2) ethyl acetate - methanol - water - acetic acid 100:2.7:5:3; 3) ethyl acetate - methanol - water - formic acid 100:2.7:5:3; 4) ethyl acetate - isopropanol - ammonia 100:2:1. RP TLC with water - methanol 7:3 containing 0.25 % ammonia; development in horizontal chamber (sandwich configuration) at room temperature. Two-dimensional TLC with mobile phase 2 in the first direction and, after drying, with mobile phase 3 in the second direction. Evaluation under UV 254 nm and after derivatization with iodine reagent or with Dragendorff reagent. TLC separations were documented by scanning. Silica and cyanopropyl layers with acidic aqueous mobile phases gave the best separation.

J. Planar Chromatogr. 17, 300-304 (2004). TLC of essential oils and thymol, carvacrol, geraniol as standards and streptomycin and gentamycin as positive controls on silica gel with toluene - ethyl acetate 93:7. Detection with ethanolic vanillin sulfuric acid. Quantitative determination at 500 nm. For bioautography the developed plates were dipped for 10 s in approximately 50 mL culture medium containing the test organism followed by drying for 2 min. After storage of the plates at 26 - 28 °C for 17 h they were dipped for 10 s in an aqueous solution (0.1 g / 60 mL) of 3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyltetrazolium bromide (MTT) the layers were incubated at 28 °C for 2 h and then dipped in 70 % ethanol and dried at room temperature.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 225-227 (2004). TLC on silica gel with 1) petroleum ether (30 - 60 ºC) - ethyl acetate 9:1; 2) benzene - glacial acetic acid 4:1. Detection 1) by spraying with 1 % vanillin solution and heating at 105 ºC for 10 min; 2) under UV 365 nm. Identification by fingerprint technique. Quantification of ferulic acid by densitometry at 325 nm. Validation of the method by investigation of linearity (0.16 µg - 1.6 µg, r = 0.9992); precision (RSD = 1.8 %, n= 5 within plate and RSD = 2.3 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 0.5 %); and standard addition recovery (97.17 %, RSD = 0.9 %, n = 5). The results for three real life samples are given.

J. Planar Chromatogr. 18, 364-367 (2005). HPTLC of ephedrine on silica gel in a presaturated twin-trough chamber with toluene - diethyl acetate - diethylamine 7:2:1. Quantitative determination by absorbance measurement at 200 nm. Also HPTLC of methanolic extracts of roots and aerial parts of different Sida species with toluene - chloroform - ethanol 13:30:7. Common and distinguishing bands were observed.