J. Planar Chromatogr. 30, 429-439 (2017). HPTLC of physalin L in orange husks of Physalis alkekengi L. var. franchetii on silica gel with ethyl acetate – n-hexane 3:2. Detection by dipping into 2.5 % (v/v) sulfuric acid in ethanol. Qualitative determination under UV 366 nm. The hRF values for physalin L and its impurity were 61 and 51, respectively, as determined by HPTLC-MS.

Rev. Bras. Farmacogn. 28/1, 92-101 (2018). HPTLC fingerprint of Eugenia uniflora on silica gel with ethyl acetate – formic acid – water 18:1:1. Detection by spraying with natural products reagent followed by PEG reagent. Qualitative identification under UV 365 nm. The hRf values of gallic acid, myricetrin and ellagic acid were 71, 34 and 38, respectively.

J. Planar Chromatogr. 31, 197-201 (2018). HPTLC of shatavarin IV in the roots of Asparagus racemosus on silica gel with ethyl acetate – methanol – water 15:3:2. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 425 nm. The hRf value for shatavarin IV was 43. Linearity was in the range of 600-1800 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 14 and 44 ng, respectively. Average recovery was 96.2 %.

J. Chromatogr. Sci. 56, 518-523 (2018). Presentation of a validated method for the quantitation of dihydrokaempferol-4′-O-glucopyranoside. This flavanonol glucoside is a marker compound in the flower of Alcea rosea L. with significant antioxidant and anticancer activity against the HepG-2 cell line. HPTLC on silica gel with ethyl acetate – methanol – water – acetic acid 600:100:80:3 over 70 mm with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 295 nm. The amount of dihydrokaempferol-4′-O-glucopyranoside in the flowers of A. rosea was 0.733 g/100 g and 0.928 g/100 g after maceration and sonication for 15 min, respectively. Linearity was in the concentration range of 0.9-3.6 µg/zone. The %RSD of the intra-day and inter-day precision was 0.2–1.5 % and 0.5–1.7 %, respectively. The LOD and LOQ were 312 ng/zone and 947 ng/zone, respectively.

Pharmacogn. Mag. 14, 45-51 (2018). HPTLC of stigmasterol in Monochoria vaginalis and Monochoria hastata on silica gel with chloroform – methanol 4:1. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for stigmasterol was 30. Linearity was between 1000 and 5000 ng/zone. LOD and LOQ were 80 and 200 ng/zone. The intermediate precision was <3 % (n=3). Average recovery was 99.8 %.

J. Chromatogr. 312, 357-385 (1984). Two-dimensional TLC of dansylated basic nitrogen fractions (BNF) on silica with cyclohexane - ethyl acetate 2:3 and with benzene - triethylamine 5:1. Detection by UV 366 nm. Dansylation of the plant amines was carried out prior to TLC with DNS chloride in acetone (5 mg/ml) in the presence of excess solid NaHCO3. Standards for comparison were separated with chloroform - butylacetate 8:3 and with cyclohexane - benzene - methanol 2:7:1. Rf values of more than 80 plant amines are reported.

Phytochemistry 25, 2910-2912 (1986). Separation of indole alkaloids and heteroyohimbine alkaloids on silica with chloroform - methanol 9:1, ethyl acetate - isopropanol - NH3 16:3:1 or chloroform - acetone 5:4. Detection with Dragendorff's reagent for alkaloids, 0.2 M FeCl3 in 35 % perchloric acid for heteroyohimbine alkaloids, 0.5 % anisaldehyde in formic acid - sulfuric acid - methanol 2:1:17 for terpenes and procyanidins and with 1 % vanillin in 50 % aq. phosphoric acid for alkaloids and terpenes. Preparative Separation by centrifugal TLC on silica with chloroform - methanol 9:1 and 4:1.

Planta Med. 57, 560-565 (1991). TLC of fourteen bufadienolides from a bulb extract of Urginea maritima on silica with chloroform - methanol - water 140:44:7 and ethyl acetate - methanol - water 81:11:8. Also HPTLC on RP-2 with methanol - water 1:1. Detection by spraying with anisaldehyde/sulfuric acid. Excellent separation of cardiotonic glycosides on RP-2 HPTLC plates. Also CC and HPLC.