CBS 91, 9-11 (2003). HPTLC on silica gel with ethyl acetate - 2-propanol - water 13:5:2 over 65 mm with chamber saturation. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 5-10 min. Visual evaluation in white light and at 254 nm.

J. Planar Chromatogr. 17, 22-25 (2004). HPTLC and TLC of caffeic acid, rosmarinic acid, caffeetannins and flavonoids on silica gel, amino-, cyano-, and RP-18-phases in a horizontal DS-chamber with a variety of mobile phases at room temperature. Acetone - acetic acid 17:3 was best mobile phase on the aminopropyl layer; water - methanol 3:2 on RP 18. Detection of the colored compounds under UV light at 365 nm before and after spraying with 2 % methanolic aluminum chloride solution, or in visible light after treatment with bis-diazotized sulfanilamide.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (7), 597-600 (2004). TLC of Qieban Zhiyang ointment extracts on silica gel with 1) benzene - ethyl acetate 10:1; 2) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 3) chloroform - methanol - ammonia 40:3:1; 4) benzene - chloroform - acetone 5:4:1; 5) chloroform - methanol 19:1. Detection 1) under UV 365 nm; 2) by exposing to ammonia vapor; 3) by spraying with potassium iodobismuthate solution; 4) under daylight. Identification by fingerprint technique. Quantification of icariine by HPLC.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (8), Append. 9-11 (2004). TLC on silica gel previously immersed in 0.5 % NaOH solution, developed with 1) ethyl acetate - methanol - water 100:17:13; and 2) with the upper phase of toluene - ethyl acetate - formic acid - water 20:10:1:1. Detection by spraying with AlCl3 solution and under UV 365 nm. Identification by fingerprint technique and comparison with the standard. Quantification of scutellarin by HPLC.

J. Planar Chromatogr. 18, 221-223 (2005). HPTLC of horminone on silica gel in a twin-trough chamber with hexane - dioxane 9:1. Absorbance measurement at 271 nm. For fluorescence analysis the plates were dipped in 1 % diphenylboryloxyethylamine in ethyl acetete for 2 s, followed by drying and dipping in a solution of 5 % PEG 8000 in dichloromethane for 2 s. After 15 min fluorescent zones of horminone were scanned at 366/>400 nm. Use of the fluorescence reagent successfully reduced the limits of detection and quantification to 0.75 ng/spot and 1.51 ng/spot, respectively. Linearity range was from 60-300 ng/spot.

Abstract CP-53, IPC (2005). HPTLC of andrographolide and wedelolactones in several market samples on silica gel with toluene - acetone - formic acid 9:6:1. Quantitative determination by absorbance measurement at 254 nm. The hRf value of andrographolide was 52 and of wedelolactone 58. Linearity was obtained between 200-400 ng/spot and 120-200 ng/spot respectively with recovery rates of 98.1-106.7 %. A complex coumarin from Eclipta alba was used as marker.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (3), 385-388 (2005). TLC of the extracts on silica gel with 1) ethyl acetate - acetone - formic acid - water 5:5:1:1; 2) chloroform - ethyl acetate - methanol - water 8:20:11:5; 3) n-hexane - ethyl acetate 9:1. Detection by spraying with 1) 5 % H2SO4 and 2) 10 % H2SO4, both in ethanol followed by heating at 105 ºC until the spots are visualized; 3) under UV 365 nm. Identification by comparison with the standard. Quantification of geniposide by HPLC. The analysis results for three real life samples are given.

Planta Med. 70, 446-251 (2004). Preparative TLC of gobicusin B and 4-[5-(4-methoxy-phenoxy)-3-penten-1-ynyl]-phenol on cellulose with chloroform (60 mL x 3) and analytical TLC on silica gel. Detection under UV light at 254 nm or by spraying with 5 % sulfuric acid in ethanol, followed by heating.