J. Planar Chromatogr. 24, 301-305 (2011). HPTLC of a methanolic extract of E. littorale Blume and isovitexin on silica gel with acetonitrile - water 3:2 at room temperature (28 +/- 2 °C) in a twin-trough chamber saturated for 30 min. Quantitative determination by densitometry at 350 nm. Linearity was between 100-400 ng/band. The %RSD for instrumental precision, intra-day precision, and intermediate precision was less than 2 %. The recovery was 99.7 %. The limit of detection and quantification was 0.6 and 1.9 ng/band, respectively.

Anal. Chim. Acta 632 (2), 168-180 (2009). Ochratoxins and aflatoxins are the most significant mycotoxins and there has been a broad range of research. However, it is impossible to use one standard technique for the analysis because of the various structures of mycotoxins. The review discusses existing analytical and detection techniques, such as 1) sample pre-treatment methods like liquid-liquid extraction, supercritical fluid extraction, or solid phase extraction; 2) separation methods such as TLC, HPLC, GC, and CE and 3) other methods such as ELISA. The practical requirements for high-sensitivity analysis and the need for a specialist laboratory setting create challenges for routine analysis. There are a number of methods used, but there is no single technique that stands out above the rest, although HPLC-MS is popular. Discussion of further currents trends, advantages and disadvantages and future prospects of these methods.

Journal of Pharmacy Research 4(4), 1197-1198 (2011) Vitamin C contents were estimated in fruit and fruit pulp. HPTLC of vitamin C from methanolic extracts of Pithecellobium dulce pods on silica gel with ethanol - water 2:1. Densitometric quantification at 254 nm. The method was linear in the range of 100-600 ng/band. The method could be employed for quality control of formulation containing the plant as one of the ingredient.

J. Trad. Chinese Veterinary Med. 5, 53-55 (2010). TLC of stachydrine on silica gel with acetone - ethanol - hydrochloric acid 10:6:1. Detection by spraying with bismuth potassium iodide - 1 % iron(III)chloride in ethanol 5:1 and heating at 100 ºC. Identification by comparison of the hRf value and zone color with the standard. Quantification of stachydrine by densitometry at 510 nm. Precision (%RSD within plate, n = 8) was 3.7 %. Stability of measurement (%RSD within 120 min, n = 5) was 4.5 %. Linearity was in the range of 3.2-38.3 µg/zone (r=0.997, n = 6). The recovery (by standard addition) was 96.6 % with a %RSD of 2.0 % (n = 6).

Chinese J. of Ethnomed. & Ethnopharm. 23, 61-62 (2010). Preparation of the samples by extracting Saururus chinensis (Lour.) Baill with methanol - 25 % hydrochloric acid 4:1 and sonication for 1 h (these were the best conditions of five different solvent compositions and different sonication times investigated). TLC of the obtained extracts on silica gel with 1) petroleum ether (60-90 ºC) - acetone 5:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 2) toluene - ethyl acetate - formic acid 5:2:1, detection by spraying with 1 % aluminium chloride in ethanol and evaluation under UV 366 nm; 3) hexane - ethyl acetate - formic acid 70:50:8, detection by spraying with 1 % aluminium chloride in ethanol and heating at 105 ºC, detection under daylight and UV 366 nm; 4) toluene - ethyl acetate - formic acid 5:4:1 saturated with hydrochloric acid, detection under daylight and UV 366 nm. System 4) provided the best separation and was most practical. Identification of quercetin by fingerprint comparison with the standard.

J. Planar Chromatogr. 25, 361-362 (2012). HPTLC of pigments in wheatgrass on sucrose-impregnated silica gel prepared by dipping the plates into a sucrose solution (400 g granulated sucrose/1 L distilled water), followed by heating at 110 °C for 20 min. Development with n-hexane - acetone 37:13. Detection by absorbance measurement between 400 and 800 nm. Sucrose impregnation prevents the degradation of the pigments during the analysis.

J. of Chinese Med. 26 (160), 1088-1090 (2011). Dangshi tablets are a herbal TCM preparation for cleaning heat, diuresis, and dissolving stones, and are prescribed to cure urinary tract infections and acute nephritis. For quality control, TLC of the extracts of the medicine on silica gel 1) for Poria cocos, with petroleum ether (30-60 ºC) – acetone – ethyl acetate 85:15:1, detection at UV 366 nm; 2) for Licorice, with chloroform – methanol – water 40:10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ° until the zones are visible, evaluation in daylight.

Chinese J. of Inform. on TCM 17 (12), 48-50 (2010). Fuyanshuan suppository is a herbal TCM preparation for heat clearing, detoxifying, eliminating stagnation, regulating the flow of vital energy and invigorating the circulation of blood. For quality control TLC of the extracts of the preparation 1) for Ajuga becumbens Thunb., on silica gel with cyclohexane - chloroform – ethyl acetate – glacial acetic acid 40:10:16:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C, evaluation under UV 366 nm or daylight; 2) for Lonicera japonica, on silica gel with chloroform – acetone – formic acid 3:2:1, detection by exposure to daylight for 10 min and viewing under UV 366 nm.