Chinese J. of Med. Guide 11 (16), 507-509 (2013). Xiaoxumingtang keli granule is a herbal TCM effective for promoting blood circulation and removing blood stasis, prescribed as an assistant drug for the treatment of stroke, hemiplegia，rheumatis, etc. For quality control, TLC 1) for Saposhnikovia divaricata (Trucz.) Schischk. and the standards isoacteoside and 4’-o-beta-glucopyranosyl-5-o-methylvisamminol on silica gel with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 254 nm; 2) for Panax ginseng C. A. Mey. and the standards ginsenoside Rg1, Re, and Rb1 on silica gel with chloroform – methanol 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight and under UV 366 nm; 3) for Ligusticum chuanxiong Hort. and the standards ligustrazine and ferulic acid on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 4) for Paeonia veitchii and the standard paeoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 5) for Ephedra sinica Stapf and the standard (1R,2S)-(-)-ephedrine hydrochloride, on silica gel with chloroform – methanol – concentrated ammonia 40:10:1, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C until the zones are visible in daylight; 6) for Scutellaria baicalensis Georgi and standard baicalin, on plates made of silica gel - sodium acetate – 0.3 % CMC 50:8:150, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation in daylight.

J. of China Pharm. 22 (z1), 81-82 (2013). Danhong Ruangan Jiaonang capsule is a herbal TCM for promoting blood circulation and strengthening spleen, and is prescribed clinically for the treatment of right flank pain caused by blood stasis, anorexia, abdominal distension, lassitude, etc. For quality control, TLC on silica gel 1) for Atractylodes macrocephala, with n-hexane – ethyl acetate 3:1, detection by spraying with 25 % phosphomolybdic acid in ethanol and heating until the zones are visible in daylight; 2) for Carthamus tinctorius L., with ethyl acetate – formic acid – water – methanol 35:10:15:2, detection in daylight; 3) for Angelica sinensis, with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

Phytochemistry 106, 44-54 (2014). HPTLC of phenylethanoid glycosides acteoside and aucubin in tissue cultures of Plantago lanceolata on silica gel with ethyl acetate - water - acetic acid - formic acid 134:36:15:15. Detection by spraying with 0.5 g vanillin dissolved in methanol - acetic acid - sulphuric acid 17:2:1, followed by heating at 105 ºC for 20 min. Qualitative identification at 520 nm. The hRF values of acteoside and aucubin were 20 and 70, respectively.

J. Planar Chromatogr. 27, 449-453 (2014). HPTLC of the glycome of different species of Panax (P. ginseng, P. notoginseng, and P. quinquefolium). Monosaccharides were analyzed on cellulose phase with ethyl acetate - pyridine - acetic acid - water 35:15:1:7 for the first development and 35:15:1:9 for the second development. Detection by spraying with aniline-O-phthalic acid reagent (containing 1.6 g o-phthalic acid dissolved in 100 mL water-saturated n-butanol, containing 0.9 mL of aniline), followed by heating at 105 °C for 5 min. Oligosaccharides were analyzed on silica gel with n-butanol - isopropanol - acetic acid - water 7:5:1:2. Detection by spraying with diphenylamine–aniline–phosphoric acid solution (4 g diphenylamine mixed with 4 mL aniline and 20 mL 85% (v/v) phosphoric acid in 200 mL acetone), followed by heating at 105 °C for 5 min._x000D_

Phytochemistry 106, 94-103 (2014). HPTLC of corosolic acid in Lagerstroemia speciosa on silica gel with ethyl acetate - methanol 49:1. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 580 nm. The hRF value of corosolic acid was 47. Linearity was between 20 and 100 ng/zone. The intermediate precisions were below 2 % (n=3).

Chinese J. of Northern Pharmacy 11(5), 14-16 (2014). Zhuanggu Tongluo Wan pills are a TCM preparation for the treatment of degenerative joint disease and osteoporosis. For quality control, TLC on silica gel (1) for Epimedium brevicornum Maxim. and the standard icariin, with ethyl acetate – acetone – methanol – water 20:2:3:2, detection at UV 254 nm; (2) for Paeonia veitchii and the standard paeoniflorin, with chloroform - ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible; (3) for aconitine, with chloroform - ethyl acetate 1:1 after exposure to ammonia vapor, detection by spraying with a solution of potassium iodobismuthate – hydrochloric acid – water 25:1:500 and evaluation in daylight.

Journal of Ethnopharmacology 163, 192-202 (2015). TLC of the aqueous extract (crude vs. after tannin removal on a polyamide column) of the kino (red hydrosoluble exudate) of Pterocarpus erinaceus on silica gel with s-butanol – water – acetic acid 14:5:5. Detection with vanillin-hydrochloric acid reagent revealed catechic tannins and related polyphenols as dark pink zones. These compounds showed hRfs between 0 and 40, and above 60 in the case of the crude extract, whereas they almost did not appear in the tannin-free fraction. The extracts were also submitted to HPLC-HRMS, allowing the identification of epicatechin monomer in both extracts and of oligomers in the crude extract only.

J. AOAC Int. 98, 850-856 (2015). Review of the development of planar chromatography-direct bioautography methodology, with special emphasis on its use as a biomonitoring system. HPTLC of cis-spiroether (1), trans-spiroether (2), alpha-bisabolol (3), bisabolol oxides (4) and the fluorescent coumarin derivatives herniarin (5), and umbelliferone (6) in chamomile extracts on silica gel with chloroform - acetone 99:1. Detection by dipping into vanillin–sulfuric acid reagent (40 mg vanillin, 10 mL ethanol and 200 mL concentrated sulfuric acid) followed by heating at 110 °C for 5 min. Bioassays were performed by the direct bioautographic system using a Gram-negative test bacteria (Xanthomanas euvesicatoria), the luminescence gene-tagged Arabidopsis pathogen (Pseudomonas syringae pv. maculicola) and the luminescent marine bacterium (Aliivibrio fischeri) as well as a Gram-positive soil bacterium (Bacillus subtilis).