J. Planar Chromatogr. 25, 314-319 (2012). HPTLC of 4-odemethylpodophyllotoxin (1), podophyllotoxin (2), kaempferol (3), podophyllotoxone (4), and deoxypodophyllotoxin (5) in the rhizomes of Podophyllum hexandrum on silica gel with toluene - ethyl acetate 2:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 1-8 µg/band for (1), (2) and (4) and 2-10 µg/band for (3) and (5). The intermediate/inter-day/intra-day precision was below 2 %. Average recovery for all (1) to (4) were between 96.4 and 101.8 %.

J. Planar Chromatogr. 25, 420-425 (2012). HPTLC of juglone (1), quercetin (2), myricetin (3), rutin (4), caffeic acid (5), and gallic acid (6) in the stem bark of Juglans regia L. on RP-18 with methanol - water - formic acid - acetic acid 61:58:3:3. Quantitative determination by absorbance measurement at 254 nm. The hRf values of compounds (1) to (6) were 16, 20, 31, 42, 55 and 78, respectively. Linearity was in the range of 13-800 ng/zone for (1) and (5), 100-1000 ng/zone for (2) and (6) and 50-800 ng/zone for (3) and (4). Limits of detection and quantification were 4 and 13 ng/zone for (1) and (5), 30 and 100 ng/zone for (2), 15 and 50 ng/zone for (3), (4) and (5), respectively. The intra-day and inter-day precisions for compounds (1) to (6) were in the range of 0.6-1.3 % and 0.5-1.9 %, respectively. Average recovery was between 98.6 and 101.1 %.

J. of Jinan Univ. (Natural Sci.) 33 (3), 289-293 (2012). Compound Shuyu granule is a herbal TCM preparation prescribed clinically to treat insomnia, neurosis and melancholia. TLC of the extracts of the medicine on silica gel 1) for Caulis Polygoni Multiflori and the standard emodin, with the upper phase of petroleum ether (60-90 ºC) – formyl acetate – formic acid 15:5:1, detection under UV 366 nm and by exposiure to iodine vapors and viewing under UV 366 nm; 2) for Salvia miltiorrhiza and the standard salvianolic acid B, with cyclohexane – dichloromethane – acetone – formic acid – glacial acetic acid 2:4:2:2:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 ° until the zones are visible; 3) for white Paeony root, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are seen.

Chinese J. of Herald of Med. 31 (10), 1344-1347 (2012). Compound Bibao tablet is a herbal TCM preparation prescribed clinically to treat chronic rhinitis, nasosinusitis and migraine. For quality control a method for the identification of the main ingredients and determination of volatile oil in the preparation has been established. TLC on silica gel 1) for Licorice, with ethyl acetate – acetic acid – water 18:2:1 with chamber saturation with the mobile phase for 15 min, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly until the zones are visible, evaluation in daylight and under UV 366 nm; 2) for Asarum sieboldi Mig., with petroleum ether (60-90 ºC) - ethyl acetate 3:1 with chamber saturation with the mobile phase for 15 min, detection at UV 254 nm; 3) Flos Magnoliae liliflorae Buds, with petroleum ether (60-90 ºC) – ethyl acetate – formic acid 15:5:2 with chamber saturation with the mobile phase for 15 min, detection under UV 366 nm.

J. of China Pharm. 21 (3), 22-23 (2012). Rubingxiao tablet is a herbal TCM preparation prescribed to treat mastitis and hyperplasia of mammary glands. Quality control by qualitative identification of its major component drugs and quantitative determination of tetrahydropalmatine in it. TLC of the extracts of the preparations on silica gel 1) for Epimedium davidii Franch, with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection at UV 366 nm, followed by spraying with 1 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Astragalus mongholicus, with chloroform – methanol 10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visible, evaluation at UV 366 nm; 3) for Poria cocos, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid - ethanol 1:4 and heating at 105 °C until the zones were visible, viewing under daylight.

J. of Qilu Med. & Pharm. 31 (6), 327-330 (2012) Zhachong Shisanwei Pill is a herbal TCM preparation for invigorating the circulation of blood. TLC on silica gel 1) for Fructus Chebulae and the standard gallic acid, with chloroform – ethyl acetate – formic acid 3:2:1, detection by spraying with 2 % ferric chloride in ethanol and heating at 105 ºC until the zones are visible in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with chloroform – cyclohexane 5:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 ºC until the zones are visible in daylight; 3) for Aquilaria agallocha (Lour.) Roxb, with toluene – ethyl acetate 4:1, detection under UV 366 nm; 4) for Rhizoma Acori Tatarinowii, with petroleum ether (60-90 ºC) – ethyl acetate 8:3, detection under UV 366 nm.

J. AOAC Int. 96, 24-26 (2013). HPTLC of stevioside (1) and rebaudioside-A (2) in the leaves of Stevia rebaudiana on silica gel with ethyl acetate - ethanol - acetone - water 5:1:2:2. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 10-15 min. Quantitative determination by absorbance measurement at 580 nm. The hRf values of (1) and (2) were 34 and 28, respectively. Linearity was 1-7 µg/zone for both. LOD and LOQ were 127 and 387 ng/zone for (1) and 393 and 1191 ng/zone for (2). The interday and intra-day precisions were below 1.2 % (n=3). Recovery (by standard addition) ranged 89.6-93.9 % for (1) and 86.7-90.6 % for (2).

Chinese J. of Jiangxi Ind. (3), 68-70 (2012). Genianan capsules are a herbal TCM for treating some diseases during women’s menopause such as tinnitus, palpitation, insomnia, blood pressure instability, etc. For quality control, TLC on silica gel 1) Radix Polygoni Multiflori and Caulis Polygoni Multiflori and standard emodin, with toluene – ethyl acetate – formic acid 15:2:1, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis and the standards deoxyschizandrin and schizandrin B, with petroleum ether (30-60 °C) – ethyl acetate – formic acid 15:5:1, detection under UV 254 nm; 3) for Radix Ophiopogonis, with chloroform – acetone 22:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 4) Rhizoma Curculigins and the standard curculigoside, with ethyl acetate – methanol – formic acid 100:10:1, detection by spraying with 0.1 mol potassium ferricyanide – 0.1 mol ferric chloride 1:1 and viewing in daylight.