J. Planar Chromatogr. 34, 95-102 (2021). HPTLC of solasodine (1) and diosgenin (2) in different parts of Solanum xanthocarpum on silica gel with toluene - ethyl acetate - diethyl amine 60:20:3. Detection by dipping into anisaldehyde - sulfuric acid. Quantitative determination by absorbance measurement at 540 nm for (1) and 440 nm for (2). The hRF values for (1) and (2) were 38 and 45, respectively. Linearity was between 0.4 and 2.0 µg/zone for (1) and 0.1 and 0.5 µg/zone for (2). Intermediate precision was below 4 % (n=3). The LOD and LOQ were 524 and 1588 ng/zone for (1) and 523 and 1596 ng/zone for (2). Average recovery was 100.7 % for (1) and 100.2 % for (2).

J. Planar Chromatogr. 34, 61-69 (2021). HPTLC of quercetin (1), gallic acid (2), eugenol (3) and β-sitosterol (4) in Psidium guajava on silica gel with toluene - ethyl acetate - formic acid 7:3:1 for (1) and (2) and toluene - ethyl acetate - formic acid 18:2:1 for (3) and (4). Detection by spraying with vanillin reagent. Quantitative determination of (1) - (4) by absorbance measurement at 254 nm, 370 nm, 570 nm and 600 nm. The hRF values for (1) to (4) were 52, 28, 87 and 59, respectively. Linearity was between 1 and 5 µg/zone for (1) and 2 and 10 µg/zone for (2) to (4). Intermediate precision was below 5 % (n=3). The LOD and LOQ were 535 and 1621 ng/zone for (1), 1059 and 3210 ng/zone for (2), 1034 and 3134 ng/zone for (3) and 1059 and 3210 ng/zone for (3), respectively. Average recovery was 100.0 % for (1) to (4).

J. Planar Chromatogr. 34, 34-45 (2021). HPTLC of labiatenic acid (synonym: rosmarinic acid) (1), apigenin (2) and buddleoside (3) in Hyssopus officinalis on silica gel with dichloromethane - ethyl acetate - formic acid 6:5:1. Detection by spraying with 2 % aluminum trichloride ethanol solution, followed by heating until the zones were clear. Quantitative determination by absorbance measurement at 330 nm. The hRF values for (1) to (3) were 20, 36 and 59, respectively. Linearity was between 90 and 454 ng/zone for (1), 33 and 197 ng/zone for (2) and 20 and 120 ng/zone for (3). Intermediate precision was below 4 % (n=6). Average recovery was 101.3 % for (1), 99.8 % for (2) and 100.5 % for (3).

J. Planar Chromatogr. 34, 79-87 (2021). HPTLC of lupeol in roots and stems of Hygrophila schulli on silica gel with benzene - chloroform - methanol 372:21:7. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 100 ºC for 3-5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for lupeol was 43. Linearity was between 200 and 1000 ng/zone. Intermediate precision was below 2 % (n=3). The LOD and LOQ were 21 and 63 ng/zone, respectively. Average recovery was 98.7 % in roots and 98.8 % in stems.

J. Planar Chromatogr. 34, 39-44 (2021). HPTLC of gallic acid (2) and quercetin (1) in Eclipta alba and Guiera senegalensis on silica gel with toluene - ethyl acetate - glacial acetic acid 6:3:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 58 and 76. Linearity was between 200 and 2000 ng/zone for both (1) and (2). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 3 and 10 ng/zone for (1) and 13 and 39 ng/zone for (2). Recovery was between 98.9 and 113.2 % for (1) and 100.8 and 113.7 % for (2).

J. Planar Chromatogr. 34, 55-60 (2021). TLC of genistein‑7‑O‑[α‑rhamnopyranosyl‑(1 → 6)]‑β‑glucopyranoside (GTG) in Derris scandens on silica gel with ethyl acetate - methanol - water 80:13:10. Quantitative determination by absorbance measurement at 254 nm for (1), 256 nm for (2), 300 nm for (3) and 296 nm for (4). The hRF value for GTG was 26. Linearity was between 8 and 120 µg/mL. Intermediate precision was below 2 % (n=3). The LOD and LOQ were 3 and 9 µg/mL. Recovery was between 102.3 and 108.6 %. The results obtained from the TLC–densitometric, TLC–image analysis and HPLC methods were comparable.

J. Planar Chromatogr. 34, 39-44 (2021). HPTLC of salicylic acid (1), kaempferol (2), gallic acid (3), and protocatechuic acid (4) in Cinnamomum verum on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Quantitative determination by absorbance measurement at 302 nm for (1), 256 nm for (2), 300 nm for (3) and 296 nm for (4). The hRF values for (1) to (4) were 54, 66, 31 and 37, respectively. Linearity was between 200 and 700 ng/zone for (1) and 100 and 600 ng/zone for (2) to (4). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 70 and 200 ng/zone for (1), 40 and 100 ng/zone for (2) to (4). Recovery was between 97.8 and 98.9 % for (1), 98.1 and 99.0 % for (2), 97.4 and 99.2 % for (3) and 98.4 and 99.5 % for (4).

Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).