J. Planar Chromatogr. 33, 179-189 (2020). HPTLC of plumbagin in the roots of Plumbago zeylanica on silica gel with toluene - ethyl acetate 3:1. Quantitative determination by absorbance measurement at 270 nm. The hR_F value for plumbagin was 64. Linearity was between 400 and 1200 ng/mL. Intermediate precisions were below 1 % (n=3). The LOD and LOQ were 1 and 3 ng/zone. Average recovery was found to be in the range of 99.8-100.2 %.

J. Planar Chromatogr. 33, 191-201 (2020). HPTLC of rutin in the leaves of Phoenix sylvestris on silica gel with chloroform - methanol - formic acid 10:40:1. Quantitative determination by absorbance measurement at 265 nm. The hR_F value for rutin was 71. Linearity was between 200 and 1000 ng/zone. Intermediate precisions were below 2 % (n=5). The LOD and LOQ were 125 and 205 ng/zone. Average recovery was found to be in the range of 99.0-100.0 %.

J. Planar Chromatogr. 33, 79-87 (2020). HPTLC of vitamin C in the dried fruit of Rosa laxa on silica gel with ethyl acetate - absolute ethanol - water 40:24:15. Quantitative determination by absorbance measurement at 273 nm. The hR_F value for vitamin C was 61. Linearity was between 0.25 and 1.2 µg/zone. Intermediate precisions were below 2 % (n=6). Average recovery was 98.4 %.

J. Planar Chromatogr. 33, 27-32 (2020). HPTLC of rutin (1), chlorogenic acid (2) and gallic acid (3) in the seeds of Moringa oleifera on silica gel with ethyl acetate - acetone - water - formic acid 6:3:2:2. Quantitative determination by absorbance measurement at 254 nm. The hR_F values for (1) to (3) were 51, 72 and 83, respectively. Linearity was between 1000 and 7000 ng/zone for (1), 100 and 700 ng/zone for both (2) and (3). Intermediate precisions were below 1 % (n=6). The LOD and LOQ were 5 and 17 ng/mL for (1), 7 and 22 ng/mL for (2) and 7 and 20 ng/mL for (3), respectively. Recovery rate was between 98.1 and 99.5 % for (1), 98.8 and 99.5 % for (2) and 97.6 and 98.6 % for (3).

J. Planar Chromatogr. 33, 33-41 (2020). HPTLC fingerprint of the kernel and seed of Allanblackia parviflora fruit (extracted with methanol - water 4:1) on silica gel with methanol - water - ethyl acetate 33:27:200. Detection by spraying with vanillic acid - sulfuric acid reagent (1 g of vanillic acid in 100 mL of 96 % ethanol, followed by the dropwise addition of 2 mL concentrated sulfuric acid). Different formulations of the derivatization reagent were investigated and the use of vanillic acid instead of the more frequently used vanillin provided a better result. Qualitative determination under UV 254 and 366 nm. Consistent hR_F values of the main zones under UV 254 nm were 39, 35 and 12. For the derivatized plate, consistent hR_F values of the main zones were 39, 34, 31 and 22.

J. of Chromatogr. Sci. 57 (8), 688 - 696 (2019). Tephrosia purpurea (L.) Pers., commonly known as “wild indigo”, is used in TCM to treat liver, spleen and kidney disorders. To investigate the seasonal effect on the quantity of its phytoconstituents lupeol, β-sitosterol and rotenone, analysis of the extracts from plant material collected in different seasons by HPTLC on silica gel with toluene - ethyl acetate - formic acid 9:1:1. The zones due to  β-sitosterol, rotenone and lupeol were observed at hRF 38, 45 and 52, respectively. Validation of the method in terms of precision, repeatability, specificity, sensitivity, linearity and robustness. The quantitative results obtained with this method showed that the content of these phytoconstituents varies from season to season. It was found that T. purpurea should not be collected in winter season for the preparation of therapeutic formulations because of the high content of rotenone, which is responsible for Parkinson’s disease and associated with heart failure, fatty liver and liver necrosis.

J. of Modern Trad. Chinese Med. 20 (7), 821-824 (2018). Mulusin, a class of isoprene flavonoids extracted from Mori Cortex, has anti-tumor, anti-inflammatory, hypoglycemic, hypolipidemic, analgesic, anti-spasm, and cholinesterase restricting activity. For quality control, TLC of mulusin on silica gel with petroleum ether - dichloromethane - ethyl acetate 15:8:10 at 25 ± 0.3 ˚C with chamber saturation for 30 min. Detection at UV 254 nm. The hRF of mulusin standard was 43. Quantitative absorbtion measurement of morusin by densitometry at 273 nm. Linearity was in the range of 200 - 1100 ng/zone (r=0.999), precision on one plate was RSD = 1.21 % (n=6) and on plate-to-plate RSD = 2.30 % (n=6). Recovery from standard sample addition was 99.2 % (RSD = 1.51, n = 6). The LOD was 20 ng/zone and LOQ 40 ng/zone.

J. Chromatogr. A 1594, 190-198 (2019). Quality assessment of herbal drugs, e.g. Morus alba L. root bark (sāng bái pí; SBP), using a HPTLC/bioautography method. Identification of the most bioactive constituents with radical scavenging (DPPH radical assay) and antimicrobial activities (Bacillus subtilis, Escherichia coli) of 18 different M. alba samples, supported by UPLC–MS/MS analysis. Higher both radical scavenging and antimicrobial activities were found for plant materials collected from Serbia (11 samples) compared to samples provided from China (7 samples). Confirmation of geographically different samples and recognization of their main markers responsible for differences between Serbian and Chinese samples by principal component analysis (PCA). The developed procedure allowed not only for a fast chemical profiling of the investigated samples and their unambiguous identification, but also for the disclosure of major and minor bioactive constituents present in SBP.