China Pharm. 22 (20), 21-23 (2013). Clematis L. is a traditional mongolian medicines. HPTLC identification of C. aethusifolia, C. intricate, C. brevicaudata, C. hexapeta, C. macropetala and C. mandshurica on silica gel with (A) petroleum ether (60-90°C) – ethyl acetate – acetone 9:3:1 at 25 °C and relative humidity of 50 %, detection by spraying with sulfuric acid – water 1:3, followed by heating at 100 °C until the spots were visible, or (B) chloroform – methanol – water 30:6:1 at 25 °C and relative humidity of 50 %, detection by spraying with sulfuric acid – water 1:3, followed by heating to 100 °C and viewing at UV 366 nm.

Chinese Trad. Patent Med. 36 (4), 763-766 (2014). Yinchen Siling Keli granule is a TCM preparation for the treatment of chronic active hepatitis B and liver cirrhosis, etc. For quality control, TLC on silica gel (1) for Artemisia capillaris Thunb. with petroleum ether (60-90 ˚C) – ethyl acetate – acetone 5:3:2, detection by spraying with 5 % KOH solution and evaluation under UV 366 nm; (2) for Rheum officinale Baill. and the standards emodin, rhein, and chrysophanol, with the upper phase of petroleum ether (60-90 ˚C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm. Quantitative determination of paeoniflorin, gardenoside, emodin, and chrysophanol by HPLC.

Trends in Analytical Chemistry 52 (2013) 155-169. Review and discussion of carbohydrates as functional ingredients in herbs and functional foods and their analysis with different techniques. Various references on HPTLC methods for mono-, di-, oligo-, and polysaccharides are listed (HPTLC generally on silica gel, detection with diphenylamine-aniline-phosphoric acid).

J. Planar Chromatogr. 28, 386-390 (2015). HPTLC of gallic acid (1) and berberine (2) in a polyherbal formulation on silica gel with toulene – ethyl acetate – formic acid – methanol 24:18:8:1 for (1) and ethanol – water – formic acid 90:9:1 for (2). Quantitative determination by absorbance measurement at 273 and 366 nm for (1) and (2), respectively. The hRF values for (1) and (2) were 58 and 76, respectively.

Rev. Bras. Farmacogn. 26, 1-14 (2016). HPTLC fingerprinting of three varieties of Marantodes pumilum on silica gel with chloroform - methanol 9:1. Detection by dipping into a mixture of p-anisaldehyde and sulfuric acid reagent, followed by heating at 100 ºC for 10 min. Qualitative evaluation under UV light at 254 and 366 nm. M. pumilum var. pumila leaves differ from the other two varieties based on the presence of an orange band at hRF 22 or 56. The stem extract of M. pumilum var. alata had the least intense orange band at either hRF 22 or 56. A peak at hRf 22 appeared as a major compound of the stem of M. pumilum varieties in the order of var. lanceolata > var. pumila > var. alata, and can be used for quality control and identification of different parts of the plant materials.

Rev. Bras. Farmacogn. 26, 197-202 (2016). HPTLC of tannins (1), flavonoids (2), anthraquinones (3), terpenes (4), cardiotonic glycosides (5) and alkaloids (6) in Myracrodruon urundeuva on silica gel with toluene – acetic acid – formic acid 70:167:14 for (1), ethyl acetate – methanol – water 8:1:1 for (2), toluene – acetone – chloroform 8:5:7 for (3), hexane – ethyl acetate 1:1 for (4), ethyl acetate – methanol – water 20:3:2 for (5), and ethyl acetate – formic acid – acetic acid – water – ethyl methylketone 86:16:23:47:78 for (6). Detection by spraying with 1 % potassium ferricyanide – 2 % iron(III)chloride 1:1 for (1), 2 % aluminium chloride for (2), 5 % potassium hydroxide for (3), anisaldehyde-sulfuric acid reagent for (4), Kedde reagent for (5) and Dragendorff reagent for (6).

J. Planar Chromatogr. 29, 209-215 (2016). HPTLC of berberine in Tinospora cordifolia on silica gel with methanol – acetic acid – water 8:1:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value of ranolazine was 71. Linearity was in the range of 120-360 ng/zone. Intermediate precisions were below 2 %. The LOD and LOQ were 40 and 120 ng/zone, respectively. The average recovery was 98.6 %.

Planta Medica 82 (15), 1351-1358 (2016). The barks of forest trees (Acer pseudoplatanus, Alnus glutinosa, Fagus sylvatica, Fraxinus excelsior, Larix decidua, Picea abies, Populus robusta, Populus tremula, Prunus avium, Quercus robur) were successively extracted with n-heptane, methanol, and methanol/water; extracts and gentamycin were applied on TLC plates (but not developed; no TLC), which were sterilised, covered with a Mueller-Hinton agar medium containing a Staphylococcus aureus CIP 53.154 suspension, incubated at 37°C for 24 h, and revealed with MTT. All the methanolic extracts were active, as well as some other, the most active being those of Q. robur, L. decidua, and P. abies.