J. Planar Chromatogr. 31, 445-450 (2018). HPTLC of benzyl isothiocyanate in the root of Salvadora persica with n-hexane. Quantitative determination by absorbance measurement at 254 nm. The hRF value for benzyl isothiocyanate was 39. Linearity was between 3–15 µg/zone. LOD and LOQ were 1 and 3 μg/zone. The intermediate precision was <2 % (n=3). Recovery was between 95.2 and 103.2 %.

Phytochemistry L4, 1245-1248 (1985). TLC of iridoid glucosides on silica with dichloromethane - methanol - water 70:30:3 or 80:20:2. Detection with vanillin/sulfuric acid. Also two-dimensional TLC on silica with 1) dichloromethane - methanol - water 70:30:3 and 2) butanol - methanol -water 4:1:5. Solvent systems suitable for other terpenoid glycosides such as saponins.

Phytochemistry 29, 247-249 (1990). TLC of various coumarin glycosides on silica with chloroform - methanol solvent mixtures. Detection by UV 254 and 365 nm. Preparative TLC of acetylated derivatives on silica with concentrating zone (layer thickness 2 mm) with benzene - methanol 20:1. Also column chromatography.

J. Mie Prefect. Inst. Pub. Health (Mie-Ken Eisei Kenkyusho Neupo) 36, 54-60 (1992). TLC of harmane alkaloids on silica with benzene - chloroform - ethanol with benzene - chloroform - ethanol 4:1:1. Quantification of harmane by HPLC.

Planta med. 68, 813-817 (2002). Analytical and preparative TLC of calcaratarin D and E on silica gel with n-hexane - ethyl acetate 23:2 and 43:7 by 2-fold development. Visualization under UV light at 254 nm and by spraying with 10 % molybdic acid reagent followed by heating.

Planta med. 68, 896-900 (2002). TLC of cichoric acid, echinacoside, maackianin, and umbelliferone on silica gel with ethyl acetate - AcOH - acetic acid - water 100:11:11:27 and formic acid - ethyl formate - toluene 3:10:10. Detection after spraying with 1 % 2-aminophenyl diphenyl borate in methanol and 5 % PEG 400 in methanol under UV light at 366 nm.

CBS 91, 12-13 (2003). HPTLC of flavonoids with tetrahydrofuran - toluene - formic acid - water 16:8:2:1, diterpenes with toluene - ethyl acetate 9:2, and iridoids with ethyl acetate - methanol - water 77:15:8 (with chamber saturation), in horizontal developing chamber over 52 mm. Detection of flavonoids by dipping warm plate in natural products reagent (0.5 % in ethyl acetate) followed by dipping in PEG 400 solution (5 % in dichloromethane), of diterpenes by dipping in 10 % methanolic sulfuric acid followed by heating at 105 °C, and iridoids by dipping in 4-dimethylaminobenzaldehyde reagent (1 % in 1 N methanolic HCl). Visual evaluation at 366 nm (flavonoids), white light (diterpenes), and white light with sharp cut filter 560 nm (iridoids). Stability of extracts was investigated under drastic stress conditions (acid, base, light, heat, humidity).

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (4), 343-345 (2004). TLC of ephedrine chloride on silica gel with chloroform - methanol - ammonia 200:35:6. Detection by spraying with 0.5 % ninhydrin in ethanol followed by heating at 105 ºC for a few minutes. Quantitative determination at 510 nm. Validation of the method by investigation of linear range of the calibration curve (0.42 - 2.10 µg/spot, R = 0.999), precision (RSD = 2.1 %, n=6 within plate and 2.7 %, n = 5 plate-to-plate), reproducibility (RSD = 2.3 %, n = 5), and recovery (98.1 %, n = 6). The results obtained by using the method are given towards some real-life samples.