Abstract No. C-338, 61 IPC (2009). HPTLC of gallic acid and piperine in combined formulation on silica gel with toluene - ethyl acetate - formic acid 16:8:1. Quantitative absorbance measurement at 320 nm. The method was linear in the range of 200-800 ng/band for gallic acid and 50-350 ng/band for piperine.

J. Planar Chromatogr. 23, 180-183 (2010). TLC of chamomile extracts on silica gel with benzene - ethyl acetate 19:1 or chloroform - methanol - water 40:10:1 in an unsaturated chamber. Detection at 254 and 366 nm. For bioautographic evaluation bioluminescent Bacillus subtilis or Pseudomonas syringae pv. maculicola were used; visualization by dye a reagent was achieved by dipping the plate in an aqueous solution of MTT. Quantitative determination by densitometric scanning at 300 nm (before biological detection) or at 590 nm (after visualization of the bioautogram with MTT).

International Seminar on Herbal Drug Research, PN-024 (2009). HPTLC of catechins and epicatechin in leaves of tea (Camellia sinensis on silica gel with toluene - ethyl acetate - formic acid 7:5:1. Quantitative determination by absorbance measurement at 254 nm. The leaves were found to contain 10-24 % of total polyphenols.

J. Sci. Res. 1(1), 121-127 (2009). An HPTLC method is described for qualitative and quantitative estimation of sinapic acid in Lepidium sativum (Brassicaceae). Methanolic extracts of the plant material were subjected to chromatographic separation on HPTLC silica gel with n-butanol - acetic acid - water 4:1:5. Derivatization with anisaldehyde sulfuric acid reagent, followed by heating at 110 °C for 10 min. Denstiometric quantification at 326 nm. Identification was confirmed by comparison of hRf-values of sample and standard.

Analytical Chemistry - An Indian Journal 9(2) (2010) (without page number). An HPTLC method has been reported for estimation of beta-sitosterol in whole plant powder of Tridax procumbens. HPTLC on silica gel with toluene - ethyl acetate - glacial acetic acid 40:10:1. Methanolic extracts of the plant material were used. For detection the plate was sprayed with anisaldehyde - sulfuric acid reagent. Densitometric quantification at 550 nm. The method was linear in the range of 10.0-70.0 µg/band. The plant material was found to contain 0.04 % of beta-sitosterol. The proposed method was suitable for routine quality control of plant as well as quantification of beta-sitosterol .

J. Tech. & Sci. of Ningxia Agr. & Forest, 5, 38-39 (2010). For liquorice TLC on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC. For Rhizoma zingiberis TLC on silica gel with cyclohexane - diethyl ether 1:1, detection by spraying with 10% vanillin in sulfuric acid and heating at 105 ºC, followed by evaluation in daylight.

International Journal of Pharma and Bio Sciences 1(1), 2-4 (2010). TLC of methanol 50 % extracts of cut dried flowers of Calendula officinalis on silica gel with chloroform – methanol 19:1. The hRf value of quercetin was 43. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 1-5 µg/band. The identity of quercetin in the sample was confirmed by comparing hRf values and UV spectra of sample and standard.

Acta Chromatographica 22 (3), 491-497 (2010). HPTLC of lupeol (methanolic Soxhlet extract from the bark of Mimosoups elengi) on silica gel with toluene – ethyl acetate – formic acid 12:2:1. The hRf value of lupeol was 64. Evaluation by densitometry at 220 nm. The linearity was in the range of 1–4 µg/band. The precision was 1.06 and 1.03 %RSD, respectively. Recovery was 97.3 %.