Chinese J. Ethnomed. Ethnopharm. 1, 55-66 (2011). TLC of the extracts of Baibanting 1) for Gardenia jasminoides Ellis, on silica gel with ethyl acetate - acetone – methanol – water 5:5:1:1, detection under daylight and by spraying with 10 % sulfuric acid in ethanol and heating at 110 ºC; 2) for Cuscuta chinensis Lam, on polyamide phase with methanol - glacial acetic acid - water 4:1:5, detection by spraying with 3 % alumnium chloride in ethanol, evaluation under UV 366 nm; 3) for Malaytea scurfpea fruit, on silica gel with n-hexane - ethyl acetate 4:1, detection by spraying with 10 % KOH in methanol and evaluation under UV 366 nm. Quantification of gardenoside by HPLC. The procedures proved to be simple, accurate, reproducible, robust and suitable for the quality control of the medicine.

Planta Med. 74, 352-353 (2008). HPTLC of tetraphyllin, turneradiffusin, beta-arbutin, terniflorin, echinaticin, turneradin and methanolic extracts of Turnera diffusa on silica gel with ethyl acetate - acetic acid - water 190:10:1. Quantitative determination by densitometric absorbance measurement at 254 nm.

J. Planar Chromatogr. 24, 257-263 (2011). HPTLC of hydrophilic and lipophilic constituents of Salvia miltiorrhiza and standards protocatechuic acid and aldehyde, salvianolic acid A and B, dihydrotanshinone I, rosmarinic acid, caffeic acid, cryptotanshinone, tanshinone II A, tanshinone I, and miltirone on silica gel with dichloromethane - ethyl acetate - formic acid 11:12:5 for the first development and petroleum ether - ethyl acetate - cyclohexane 15:11:14 for the second development with chamber saturation for 30 min. The first mobile phase separated the hydrophilic constituents salvianolic acid B, salvianolic acid A, rosmarinic acid, caffeic acid, protocatechuic acid, and protocatechuic aldehyde. Detection under UV light at 254 and 365 nm. After documentation the plates were placed in a second chamber and development with the low polarity mobile phase which separated dihydrotanshinone I, cryptotanshinone, tanshinone I and II A, and miltirone. Detection under UV light at 254 and 365 nm. Quantitative determination by densitometry in absorbance mode at 260 or 290 nm. The linear range was between 0.1-0.3 and 0.7-8.3 µg/zone. Instrumental precision was less than 4 % (n = 6). Precision on one plate was below 5 % (n = 6) and on different plates below 14 %. Depending on the substance, the limits of detection and quantification were between 14-22 and 69-276 ng/zone, respectively. The repeatability (n = 6) was between 1.3-3.4 %. Some of the compounds had similar hRf values: for rosmarinic acid 44, for salvianolic acid 43, for caffeic acid 49, for protocatechuic acid 49, for dihydrotanshinone 65 and for cryptotanshinone 63. Additional detection by spraying with 5 % sulfuric acid in ethanol.

leaves and its geographical variation using HPTLC fingerprint. Trends in Natural Product Research, NRP-2011 abstract No. SNP-NRP-11/069. TLC of ferulic acid in ethanolic extracts of Ricinus communis leaves on silica gel with chloroform - methanol 19:1. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 300-900 ng/band. LOD and LOQ were 4 and 11 ng/zone, respectively. The plant collected from different geographic locations showed variations in the amount of ferulic acid. The content of ferulic acid was 2.87 µg/g in leaves collected from Delhi, which was higher than those from Guwahati and Jhansi.

J. Planar Chromatogr. 24, 306-311 (2011). HPTLC of ethanolic leaf extracts of A. squamosa, with rutin and isoquercitrin as standards, on silica gel (prewashed with methanol) with ethyl acetate - formic acid - glacial acetic acid - ethyl methyl ketone - water 50:7:3:30:10 with chamber saturation for 30 min at room temperature (25 +/- 2°C) and a relative humidity of 50 +/- 5 %. Quantitative determination by densitometry at 366 nm. Linearity was between 200-1600 ng/band for both rutin and isoquercitrin. The robustness of the method (%RSD) was 1.9-2.9 % for rutin and 1.5-2.3 % for isoquercitrin, respectively. The instrumental precision (%RSD) was 0.9 and 0.3 % for rutin and isoquercitrin, respectively. The intra-day and inter-day precision (%RSD) was less than 3 % in all cases. LOD and LOQ was 75 and 100 ng/band for rutin and 40 and 80 ng/band for isoquercitrin, respectively. The hRf value was 32 for rutin and 58 for isoquercitrin. Recovery (by standard addition) was found to be 96-107 %.

Journal of Pharmacy Research 4(5), 1353-1355 (2011). HPTLC of methanolic extracts of Aegle marmelos fruit pulp on silica gel with toluene - ethyl acetate - glacial acetic acid 70:30:1. Densitometric quantification of marmelosin at 310 nm. The method was linear in the range of 50-350 ng/band. The method was used to determine the marmelosin content of pulp, unripe pulp, seeds, leaves, rind, outer stain and inner stain.

Phytochem. Anal. 24, 1-24 (2013) The application of chemometrics in combination with chromatographic fingerprining was reviewed. The authors described how this combination allowed to find correlation between different variables such as molecular profile and genetic variability and their geographical origins and growing conditions.

Hebei J. Trad. Chinese Med. 34 (3), 433-434 (2012). Compound Kuhuang lotion is a TCM preparation used clinically for clearing heat and eliminating dampness, eliminating stagnations of blood, and relieving pains. Description of a method for the quality control of the preparation. TLC of the extracts of the preparations on silica gel 1) for Rheum officinale and the standards emodin, rhein, chrysophanol and aloe emodin, with petroleum ether (60-90 ºC) – ethyl acetate – formic acid 15:5:1, detection in daylight; 2) for Radix Sophorae flavescentis and the standard matrine, with ethyl acetate – propanone – benzene – ammonia 30:10:30:1, detection by spraying with a solution of potassium iodobismuthate – water – hydrochloric acid 10:200:1, detection in daylight; 3) for Golden Cypress and the standard berberine, with benzene – ethyl acetate – methanol – isoamyl alcohol – water 20:10:5:5:1, detection at UV 366 nm.