Phytochem. Anal. 24, 395-400 (2013). HPTLC bioautography of the oleo gum-resin galbanum from Ferula gummosa Boiss on silica gel with chloroform - ethyl acetate - methanol 50:5:1 and detection under UV 366 nm. Bioautographic assay to detect the zones inhibiting acetylcholinesterase (AChE). The zones that showed activity in the assay on AChE inhibition were selected for HPTLC-MS. Auraptene and farnesiferol A were identified as AChE Inhibitors.

J. of Inner Mongolia Univ. for Nationalities (Natural Sci. Edit.) 27 (1), 80-83 (2012). Yuzan Qingyan Shiwuwei pills are a traditional Mongolian herbal medicine for treating colds, sore throat, asthma, cough, etc. For quality control, TLC on silica gel 1) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with cyclohexane – formic acetate – formic acid 15:5:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly, viewing in daylight; 3) for Radix Glycyrrhizae and the standard glycyrrhetinic acid, with benzene – petroleum ether (30-60 °C) – ethyl acetate – glacial acetic acid 50:25:20:3, detection under UV 366 nm.

Chinese J. Ethnopharm.(9), 36-38 (2012). Luman-12 is a traditional Mongolian herbal medicine preparation for treatment of cough, asthma, etc. For quality control of the main component herbal drugs TLC on silica gel 1) for Radix Polygalae, with toluene – ethyl acetate – formic acid 28:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Glycyrrhizae, with toluene – ethyl acetate – formic acid 10:8:1, detection under UV 366 nm; 3) for Piper nigrum, with benzene – ethyl acetate – acetone 7:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. Mod. Drug Appl. 7 (1), 118-119 (2013). Zhiheng tablets are a herbal TCM for treatment of hyperlipidemia. For quality control, TLC on silica gel 1) for Radix Notoginseng, with dichloromethane – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Salvia miltiorrhiza and the standard salvianolic acid A sodium, with dichloromethane – acetone – formic acid 25:10:4, detection by exposing to ammonia vapors for 10 min and viewing under UV 366 nm; 3) for Pericarpium Citri Reticulatae and the standard hesperidin, with ethyl acetate – methanol – water 100:17:13 to a distance of 3 cm and then with toluene – ethyl acetate – formic acid – water 20:10:1:1 to a distance of 8 cm, detection by spraying with 3 % aluminium chloride in ethanol and viewing under UV 366 nm; 4) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. of Medication & Clinics 12 (11), 1445-1446 (2012). Xiaocaihu tablet is a herbal TCM for treating chills and fever, dry throat, etc. For quality control TLC on silica gel 1) for Bupleurum chinense, with ethyl acetate – methanol – water 12:2:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % sulfuric acid in ethanol and heating mildly, viewing in daylight; 2) for root of Pilose Asiabell, with n-butanol – glacial acetic acid – ethyl acetate – water 14:2:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C, viewing in daylight.

J. of Fujian Univ. of TCM 22 (5), 52-54 (2012). Coix seed is a TCM herb frequently prescribed to treat edema, dermatophytosis, spasm and diarrhea. For quality control of the crude drug available from different places of origin, TLC of the herb extract and standard glycerol trioleate on silica gel with petroleum ether (60-90 °C) – ethyl acetate – glacial acetic acid 25:5:1, detection by spraying with 0.5 % vanillin in sulfuric acid – ethanol 1:200, heating at 105 °C for 5 min and viewing under UV 366 nm. Coix seed from different places of origin contains different amounts of glycerol trioleate (measured by HPLC).

J. Liq. Chromatogr. Relat. Technol. 37, 61-72 (2014). HPTLC of khellin in the fruits of Ammi visnaga on silica gel with chloroform - acetone 9:1. Quantitative determination by absorbance measurement at 248 nm. The hRf of khellin was 29. Linearity was between 50 and 300 ng/zone. LOD and LOQ were 10 and 25 ng/mL. Recovery (by standard addition) was in the range of 98-99 %. Intermediate intra- and inter-day precision was below 1.6 % (n=6). The results obtained were comparable with HPLC results.

Chinese J. of Med. Guide 18 (12), 82-84 (2012). Fufang Kuhuang Penwu spray is a herbal TCM preparation for treatment of eczema, skin itch and solar dermatitis. For quality control, TLC on silica gel 1) for borneol, with cyclohexane – ethyl acetate 33:7, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4, heating at 105 °C until the zones are visible in daylight; 2) for Cortex Phellodendri Chinensis and the standard berberine hydrochloride, with benzene – ethyl acetate – methanol – isopropanol – concentrated ammonia 12:6:3:3:1, detection under UV 366 nm; 3) for Rheum palmatum L., Reynoutria japonica Houtt, and the standard emodin, with petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm before and after exposure to ammonia vapors.