Inner Mongolian J. of Trad. Chinese Med. 10, 35-36 (2012). Jiangding Doukou Qufeng compound oral liquid is a herbal TCM preparation prescribed to effectively cure flatulence, loss of appetite, nausea, and vomiting. TLC of the extracts of the preparations on silica gel 1) for Cortex Cinnamomi and Foeniculum vulgare, with petroleum ether (60-90 ºC) – ethyl acetate 17:3, detection by spraying with 2,4-dinitrophenylhydrazine solution in 2N hydrochloric acid and viewing in daylight; 2) for Rhizoma Zingiberis, with petroleum ether (60-90 ºC) – chloroform – ethyl acetate 2:1:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C until the zones were visible in daylight.

J. of Modern Trad. Chinese Med. 14 (2), 12-14 (2012). Rubus idaeus, the dried immature fruits of Rubus chingii Hu, is a herbal TCM drug specially effective for tonifying the kidney, controlling nocturnal emission, nourishing the liver, and improving eyesight. It is prescribed clinically to cure spermatorrhea, night emission, enuresis, frequent micturition, sexual impotence and premature ejaculation. TLC of the extracts of the crude drug on silica gel with toluene – ethyl acetate – formic acid 9:3:1, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm.

Chinese J. of Northern Pharmacy 9 (4), 8-9 (2012). Shugan capsules are a herbal TCM herbal preparation for nourishing the liver and tonifying spleen, used for women’s climacteric symptoms. For quality control, TLC on silica gel 1) for Radix Paeoniae Alba, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 4:1 and heating mildly, viewing under daylight; 2) for Rhizoma Cyperi, with n-hexane - ethyl acetate 17:3, detection under UV 254 nm.

f.) Wall. ex Nees. (Chinese). Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (6), 1411-1412 (2012). The extracts of Andrographis paniculata, a traditional Chinese medicinal herb, have been widely used as the core ingredient in a variety of traditional Chinese patent medicines, such as Chuanxinlian pills, Chuanxinlian capsules, Lianzhi pills, and some cosmetics and veterinary drugs. The quality of the extracts became significant for guaranteeing the validity, security, stability and homogeneity of the medicines. TLC of the herb extracts on silica gel with chloroform – ethyl acetate – methanol 20:15:2. Detection under UV 254 nm and by spraying with 2 % 3,5-dinitrobenzoic acid in ethanol – 2 mol/L KOH solution 1:1 followed by evaluation in daylight.

J. Planar Chromatogr. 26, 125-131 (2013). HPTLC of positional isomers ursolic (1) and oleanolic (2) acids and their structural isomer betulinic acid (3) in the leaves or whole fruits of fresh vegetables on RP-18 phase with n-hexane - ethyl acetate 5:1. Qualitative identification by absorbance measurement at 366 nm. The hRf of compounds (1) to (3) were 42, 48 and 62, respectively.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (1), 94-96 (2012). Astragalus as a widely used medicinal raw drug is the dried root of Astragalus membranaceus (Fisch.) and Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao. Its pharmacological effects are nourishing the vitality, invigorating splenic yang, promoting tissue regeneration, inducing diuresis for removing edema, and invigorating the circulation of blood. The chemical constituents of the extracts of astragalus mainly are saponins, polysaccharides and flavones. TLC of the astragalus extracts on silica gel with the lower phase of methanol – chloroform – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation in daylight and under UV 366 nm. By comparison with standards the markers astragaloside I-Vlll, acetylastragaloside, isoastragaloside I, II, lV, and soyasaponin I were identified.

J. Anhui Med. & Pharm. 17 (2), 202-203 (2013). Banlangen granule, processed of Radix Isatidis, is a traditional Chinese herbal medicine prescribed clinically to treat sore throat, cheek swelling, acute and chronic tonsillitis and mumps. For quality control a method for the determination of leucine, a main antiviral effective component, is presented. TLC on silica gel with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C and viewing in daylight. Quantification by densitometry at 509 nm. Validation of the method by investigation of the linearity range (0.10-0.90 µg/zone, r = 0.998, n=5), precision (%RSD = 2.8 %, n=6), stability (%RSD = 0.8 %, n=6, towards single lane within one hour), and reproducibility (%RSD = 2.0 %, n=6, within plate). The recovery (by standard addition) was 98.8 % (%RSD = 1.1 %, n=6).

J. of Guangzhou Chem. Engin. 41 (9), 146-147 (2013). Shuangxiang Hewei capsules are a herbal TCM preparation and contain Pogostemon cablin (Blanco) Benth. as the key drug, which is used for treatment of anorexia, vomiting, gastric distention, and limb weakness. For quality control through identification of the key drug Pogostemon cablin, TLC of the drug extracts and the standard patchouli alcohol on silica gel with cyclohexane – acetone 10:3, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight.