J. Planar Chromatogr. 27, 69-71 (2014). HPTLC of bergenin (1) and gallic acid (2) in the rhizome of three Bergenia species, B. ciliata, B. stracheyi, and B. ligulata on silica gel with toluene - ethyl acetate - formic acid 7:11:2. Quantitative determination by absorbance measurement at 314 nm. The hRF values for (1) and (2) were 25 ad 59, respectively.

Chinese J. of Clin. Res. on Trad. Chinese Med. 5 (18), 38-40 (2013). Huaiyu Wan pill is a herbal TCM for treating bleeding of rectum polyp, anal fissure, hemorrhage due to ulcerative colitis etc. For quality control, TLC on silica gel 1) for Gardenia jasminoides Ellis and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones are visible in daylight; 2) for flowers and buds of Sophora japonica L. and the standard rutin, with ethyl acetate – formic acid – water 8:1:1, detection by spraying with 1 % aluminum trichloride in ethanol, detection under UV 366 nm; 3) for Paeonia suffruticosa Andr. and the standard paeonol, with cyclohexane - ethyl acetate 3:1, detection by spraying with 2 % ferric chloride in ethanol and heating until the zones are visible in daylight. Quantification of rutin by HPLC.

J. Trad. Chinese Veterinary Med. 47 (9), 32-33 (2012). In veterninary TCM Cangzhu Xianglian San powder is prescribed for the treatment of dysentery and diarrhea of livestocks. For quality control TLC on silica gel 1) for Atractylodes lancea, with petroleum ether (60-90°C) – acetone 9:2, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly until the zones are clearly visible in daylight; 2) for Radix Aucklandiae, with cyclohexane – ethyl acetate – formic acid 15:5:1, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly until the zones are clearly visible in daylight.

Chinese J. of Spectroscopy & Spectral Anal. 34 (4), 990-993 (2014). In recent years, some frauds were discovered on the drug market, e.g. chemical drugs with similar effect were illegally added to herbal TCM for hypertension which may cause a variety of adverse reactions and even endanger life. To ensure the safety of patients a method is presented for rapid screening of antihypertensive drugs. TLC on silica gel with dichloromethane – methanol – water 90:10:1, detection under UV 254 nm. Identification of nicardipine hydrochloride, doxazosin mesylate, propranolol hydrochloride and hydrochlorothiazide by comparison of the corresponding hRf values of standards. Qualitative analysis of the sample target zones by adding a few drops of silver glue solution onto each zone and then detection by surface enhanced Raman spectroscopy (SERS) using a portable Raman spectrometer at the integration time of 10 s and the laser power of 100 mW with a minimum detection amount of 5, 5, 50 and 50 ng, respectively. The TLC method was optimized regarding mobile phase, sample application volume, the concentration of silver glue solution, etc. The method was used for the analysis of 10 commercial products from the drug market. Two products contained illegally added nicardipine hydrochloride and doxazosin mesylate, and one product contained propranolol hydrochloride and hydrochlorothiazide.

Heilongjiang Med. J. 26 (5), 746-750 (2013). Ganmaoling Jiaonang capsules are a TCM preparation composed of traditional Chinese herbal and Western drugs, with antipyretic and analgesic effects, which is used for headaches, fever, runny nose, sore throat, etc. For quality control, TLC on silica gel (1) for Evodia lepta (Spreng.) Merr with water-saturated toluene – ethyl acetate – formic acid 5:4:1, detection at UV 366 nm; (2) for Herba Bidentis bipinnate and Herba Bidentis pilosae with chloroform – methanol – formic acid 35:5:2, detection by (A) exposure to ammonia vapor and evaluation under white light and (B) spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible; (3) for Ilex asprella (Hook. et Arn.) Champ. ex Benth. with benzene – acetone 9:1, detection at UV 366 nm. Quantitation of 4-acetamidophenol, chlorpheniramine maleate and caffeine by HPLC.

Chinese J. of Pharm. Research 33 (6), 328-331 (2014). Yinyanghuo Tongluo Wan pill is a TCM preparation for special treatment of diabetic peripheral neuropathy. For quality control, TLC on silica gel (1) for processed Epimedium and the standard icariin with ethyl acetate – butanone – formic acid 10:1:1:1, detection by spraying with 3 % aluminum trichloride in ethanol and heating mildly until the zones are visible at UV 366 nm; (2) for Panax ginseng C. A. Mey., ginsenoside Rb1, ginsenoside Re, and ginsenoside Rg1 with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible and at UV 366 nm; (3) for Ligusticum chuanxiong Hort. with cyclohexane - ethyl acetate 9:1, detection at UV 366 nm; (4) for Paeonia lactiflora Pall. and paeoniflorin with chloroform – methanol – ethyl acetate – concentrated ammonia 20:8:4:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until the zones are visible; (5) for Atractylodes macrocephala Koidz. with petroleum ether (60-90 °C), detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until the zones are visible; (6) for Cassia twig with petroleum ether (60-90 °C) – ethyl acetate 17:3, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating at 105 °C until the zones are visible, and at UV 366 nm.

(Rutaceae). Rapid Commun. Mass Spectrom. 29, 1530-1534 (2015). HPTLC of complex phytoconstituents in the peels of Citrus aurantium on silica gel with ethyl acetate - acetic acid - formic acid - water 100:5:5:13. Detection by spraying with vanillin reagent. Images were acquired by desorption electrospray ionization mass spectrometry in the positive ionization mode. The method allowed the identification of 20 compounds.

Planta Medica 82, 09/10, 903-909 (2016). To monitor the sorbent phase purification of a chloroform subfraction of the sponge Myxilla incrustans, TLC of fractions on silica gel with dichloromethane – methanol – formic acid 900:100:1, detection with Dragendorff’s reagent. The four positive fractions were purified by preparative HPLC, yielding three new N-acyl dopamine/dehydrotyrosine glycosides: myxillins A, B and C._x000D_