J. AOAC Int. 88, 1562-1567 (2005). HPTLC of beta-asarone (cis-2,4,5-trimethoxy-1-propenylbenzene) and alpha-asarone in Calamus rhizome on caffeine-impregnated silica gel (prepared by immmersion of conventional silica gel plates into a solution of 80 g/L caffeine in dichloromethane for 1 s followed by drying at room temperature for 5 min, then heating at 80 °C for 5 min) with toluene - ethyl acetate 93:7. Quantitative determination by absorbance measurement at 313 nm. The method was validated in terms of stability of sample during chromatography, specificity for beta-asarone, linearity (25-300 ng, including samples containing 0.05-0.7 % beta-asarone), accuracy and precision. Recovery was 100.0-100.8 %, limit of detection 6.4 ng, and limit of quantitation 12.7 ng. The method allows proper identification of Calami rhizoma raw material, and the specific, accurate, and precise quantification of beta-asarone and alpha-asarone

Planta Med. 71, 54-58 (2005). Preparative TLC of microphyllaquinone and (1aS*,1bS*,7aS*,8aS*)-4,5-dimethoxy-1a,7a-dimethyl-1,1a,1b,2,7,7a,8,8a-octahydrocyclopropa[3,4]cyclopenta[1,2-b]naphthalene-3,6-dione on silica gel with hexane - ethyl acetate 7:3 and dichloromethane - chloroform 7:3. Detection under UV light at 250 nm and by spraying with a solution of vanillin - perchloric acid - ethanol, followed by heating at 100 °C for 5 min.

Planta Med. 71, 673-679 (2005). Analytical and preparative TLC of 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester, achyranthoside C dimethyl ester, achyranthoside C butyl dimethyl ester, achyranthoside E dimethyl ester, achyranthoside E butyl methyl ester (and 10 known compounds) on silica gel and RP18 with chloroform - methanol - water 8:5:2 or methanol - water 1:1, respectively. Detection under UV light at 254 nm or by spraying with cerium sulfate - 10 % sulfuric acid 1:99.

Planta Med. 70, 1216-1221 (2004). TLC of 20-formylbenz[6,7]indolizino[1,2-b]quinolin-11(13H)one, 10-methoxy-20-O-acetylcamptothecin, 20-O-beta-glucopyranosyl-18-hydroxycamptothecin, 3,4-methylenedioxy-3’-O-methyl-5’hydroxy ellagic acid and 18 known compounds on silica gel with chloroform - methanol 4:1, chloroform - ethyl acetate 6:1, ethyl acetate - hexane 4:1, and ethyl acetate - acetone 1:4. Detection under UV light.

J. Liq. Chromatogr. & Relat. Technol. 29, 2141-2151 (2006). HPTLC of flavonoids (with rutin, chlorogenic acid, hyperoside, and gallic acid as reference substances) on silica gel in a twin-trough chamber with ethyl formate - toluene - formic acid - water 60:3:8:6. Detection by dipping the hot plate (heated at 100°C for 2 min) into natural products reagent, followed by drying, dipping into polyethylene glycol 400 (10 g in 200 mL dichloromethane), and drying. Evaluation under UV 366 nm. With this method the geographical origin of the material can be determined. Toluene - acetone - formic acid 9:9:2 allows the discrimination of green from black and other speciality teas, based on the polyphenol pattern. Detection by dipping the hot plate (heated at 100°C for 2 min) into a solution of Fast Blue salt B, followed by detection under white light. For investigation of the alkaloid profil ethyl acetate - methanol - water 20:2.7:2, followed by detection under UV 254 nm is used. The amino acid profile is analyzed by using 1-butanol - acetone - acetic acid - water 7:7:2:4. Detection by dipping into ninhydrin reagent, followed by heating at 110°C for 3 min and evaluation under white light. The method for polyphenols was validated regarding specificity, stability, reproducibility, and robustness.

Pharmazie 60, 953-955 (2005). TLC of cochleareine, acoleareine, 14-acetyltalatisamine, and talatisamine on silica gel. Detection under UV light at 254 nm. Also co-chromatography with standards. Using a Chromatotron apparatus the crude alkaloidal mixture was separated on alumina radial plates and eluted with a gradient of petroleum ether, chloroform, and methanol.

J. Liq. Chromatogr. Relat. Technol. 28, 1679-1691 (2005). HPTLC of the iridoid gycoside picroside I and picroside II on silica gel with chloroform - methanol 41:9 in a saturated twin-trough chamber. Quantitation by absorbance measurement at 290 nm.

J. AOAC Int. 90, 1203-1209 (2007). HPTLC of terpenelactones (total bilobalide, gingkolide A, and gingkolide B) on prewashed and sodium acetate preimpregnated silica gel with toluene - ethyl acetate - acetone - methanol 50:25:25:3 or ethyl acetate - hexane 9:1; also HPTLC of flavonol glycosides (quercetin, kaempferol, isorhamnetin as standards) on prewashed and preimpregnated silica gel with chloroform - acetone - formic acid - acetic acid 50:11:6:6. Plates were developed in solvent equilibrated, vapor saturated twin-trough chambers at 30°C. Densitometry in absorbance mode at 370 nm (for aglycones) and at 290 nm following a 1 s immersion in acetic anhydride and heating at different temperatures for varying lengths of time (for terpenelactones). Good relationship (95%) was determined between HPTLC and HPLC for determination of total flavonol glycosides. The HPTLC flavonol aglycone method also performed well in terms of accuracy and consecutive plate repeatability.