2nd International Conference on New Development in Drug Discovery from Natural Product & Traditional Medicine PP82, 82 (2010). Eugenia jambolana pulp was dried in vacuum and enriched by chromatography on XAD 7HP ion-exchange resin, followed by Sephadex LH 20. HPTLC of both enriched extracts on silica gel with ethyl acetate – formic acid – acetic acid – water 100:11:11:26. Quantitative determination by absorbance measurement at 520 nm. The vaccum dried pulp and the enriched extracts 1 and 2 were found to contain 0.08 %, 17 % and 10 % of anthocyanins, respectively. Malvidin-3-laminariobioside was used as marker compound for quantitative analysis.

J. Planar Chromatogr. 24, 295-300 (2011). TLC of Verbascum extracts (e. g. iridoids, and triterpene saponins) on silica gel in a horizontal chamber saturated with mobile phase for 10 min, by 2D separation with ethyl acetate - methanol - water 25 % ammonia 55:35:9:1 in the first direction and methanol - ethyl acetate - water - acetic acid 5:45:13:11 in a perpendicular direction. Detection by dipping in vanillin-sulfuric acid reagent for 1 s followed by heating for 10 min at 105 °C. The method was validated for its specifity, precision (repeatability and intermediate precision), stability, and robustness. Use of an image-processing program for the construction of an ’average’ fingerprint.

Food Research International 44, 2258-2263 (2011). HPTLC of sugar constituents linked to anthocyanidin in the fruits of Acanthopanax sessiliflorum on silica gel with n-propanol - water - 30 % ammonia 20:5:1 + 1 drop triethylamine. Detection by spraying with a solution of 0.3 % N-(1-naphtyl)-ethylenediamine and 5 % sulfuric acid in methanol, followed by heating at 121 °C for 10 min. The acidic hydrolysate consisted of the two monosaccharides, glucose and xylose.

J. Planar Chromatogr. 24, 301-305 (2011). HPTLC of a methanolic extract of E. littorale Blume and isovitexin on silica gel with acetonitrile - water 3:2 at room temperature (28 +/- 2 °C) in a twin-trough chamber saturated for 30 min. Quantitative determination by densitometry at 350 nm. Linearity was between 100-400 ng/band. The %RSD for instrumental precision, intra-day precision, and intermediate precision was less than 2 %. The recovery was 99.7 %. The limit of detection and quantification was 0.6 and 1.9 ng/band, respectively.

Asian Journal of Chemistry 23 (5), 2098-2100 (2011). HPTLC of glycyrrhizic acid in herbal formulation on silica gel with chloroform - glacial acetic acid - methanol - water 15:8:3:2. The hRf value of glycyrrhizic acid was 28. Quantitative evaluation by absorbance measurement at 254 nm. The method was found to be linear in the range of 100-500 ng/band with average recovery between 99-102 %.

J. Planar Chromatogr. 24, 77-81 (2011). HPTLC of belleric acid on silica gel, prewashed with methanol, with toluene - ethyl acetate - methanol - formic acid 15:15:7:1 in a saturated chamber at 22 °C and 65 % relative humidity. Quantitative determination by absorbance measurement at 205 nm. Average recovery was 98.7-100.9 %. Linearity was between 250 and 1250 ng/zone. Repeatability and intermediate precision (%RSD) were 1.2 and 1.5 %, respectively. LOD and LOQ were 49 and 148 ng/zone, respectively. The hRf value of belleric acid was 35.

Planta Med. 77, 742-748 (2011). Preparative TLC of two monoterpenes, two sesquiterpenes, twenty-two flavonoids, fourteen lignans, and thirty-two other compounds on silica gel with petroleum ether - ethyl acetate 5:2 and 2:1 and chloroforrm - methanol 100:1, 10:1 and 20:1.

J. Planar Chromatogr. 25, 426-432 (2012). HPTLC of shanzhiside methyl ester (1) and barlerin (2) on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 240 nm. The hRf of compounds (1) and (2) were 30 and 48, respectively. Linearity was in the range of 200-1000 ng/zone. Limits of detection and quantification were found to be 13 and 22 ng/zone for (1) and 18 and 31 ng/zone for (2), respectively. Recovery was found to be 99.2-99.5 % for (1) and 98.9-99.2 % for (2), respectively. The method showed comparable results to a validated HPLC method.