Indian J. Pharm. Sci. 69(3), 446 (2007). HPTLC of swetimarin (a phytoconstituent of Enieostemma Littorale) in chloroform extracts of a polyherbal antidiabetic commercial formulation on silica gel with benzene - methanol 4:1. Detection by spraying with 10 % methanolic sulphuric acid and heating at 130° C for 2 min. Evaluation by densitometry at 536 nm. The linearity range was 50-1500 ng/zone. Recovery was more than 96 %.

Br. J. Planar Chromatogr. 20, 65-68 (2007). HPTLC of ursolic acid on silica gel with toluene - ethyl acetate - triethylamine - methanol 7:2:1:1 in a saturated twin-trough chamber. Quantitation by densitometry at 366 nm.

by HPTLC. J. Planar Chromatogr. 21, 183-186 (2008). HPTLC of dopamine on silica gel with n-butanol - acetic acid - water 7:2:1. Detection and quantification by densitometry at 280 nm.

J. Sep. Sci. 31, 47-55 (2008). HPTLC of phyllanthin (1), hypophyllanthin (2), niranthin (3), and nirtetralin (4) in the leaves of four Phyllanthus species, P. amarus, P. maderaspatensis, P. urinaria, and P. virgatus, on a chiral TLC plate with n-hexane - acetone - 1,4-dioxane 18:2:1. Detection by dipping in vanillin reagent (1 g vanillin, 5 mL sulphuric acid, 95 mL ethanol) followed by heating at 110 °C for 15 min. Quantitative determination by absorbance measurement at 620 nm. The hRf values were 30, 36, 41, and 48 for (1) to (4), respectively. Linearity was between 100 and 500 ng/band and the recoveries for (1) to (4) were 99.9, 100.5, 99.2, 98.7 %, respectively. The limit of detection and quantification was 26 and 88, 45 and 136, 53 and 176, and 57 and 187 ng/band for (1) to (4), respectively. No significant intra- and interday variation was observed.

J. Chinese Trad. Patent Med. 30 (12), 1781-1785 (2008). TLC of Shenyi granule extracts on silica gel with 1) dichloromethane - ethyl acetate - petroleum ether (60-90 ºC) - methanol 20:14:6:1; 2) ethyl acetate - formic acid - glacial acetic acid - water 30:3:4:3; 3) toluene - ethyl acetate - formic acid 8:6:1; 4) chloroform - methanol - water 26:10:3. Detection 1) by spraying with 10 % sulfuric acid in ethanol and heating; 2) by spraying with iron(III) chloride solution; 3) by spraying with vanillin reagent. Identification by comparison with the standards glycyrrhizic acid and glycyrrhetinic acid.

Abstracts No. 9156, IHCB (2009). TLC of Hoodia gordonii on silica gel with ethyl acetate - methanol - water 36:7:5. Photodocumentation and estimation was carried out by the DISTA method. The authentication of hang-off capsules, which contained several plant ingredients used to prevent hang-over, was performed by TLC on silica gel with toluene - ethyl acetate 9:1. Detection by spraying with vanillin-sulfuric acid reagent.

J. Chromatogr. B 848 (2), 232-238 (2007). HPTLC of boswellic acids in Boswellia serrata extract and 11-keto-beta-boswellic acid in human plasma on silica gel with hexane - chloroform - methanol 10:10:1. Quantitative determination by absorbance measurement at 260 nm. The linearity range for 11-keto-beta-boswellic acid spiked in 1 mL of plasma was 29 - 146 ng/spot, with a recovery of 91.7 %. The limit of detection and quantification for 11-keto-beta-boswellic acid in human plasma was 9 and 29 ng/mL, respectively. The method was applied for the determination of 11-keto-beta-boswellic acid plasma levels in a clinical pilot study.

60th Indian Pharmaceutical Congress PG-262 (2008). HPTLC of withaferin A in Ashwagandha on silica gel aluminum layer with toluene - ethyl acetate - formic acid 5:5:1. Quantitative determination by absorbance measurement at 214 nm. The chromatograms of the tablet formulation showed a zone corresponding to standard withaferin A (hRf value 37) indicating the presence of the same in the herbal formulation. Linearity was in the range of 50-500 ng/spot. The concentration of withaferin A was 8.07 µg/tablet and 0.00134 % w/w in the tablet formulation.