International Seminar on Herbal Drug Research, PN-013 (2009). HPTLC of ethyl acetate extracts of seeds of Eugenia jambolana on silica gel with chloroform - acetone - formic acid 150:33:17. The antioxidant activity of different extract was assessed by DPPH method. The proposed method was applied to plant extract as well as to polyherbal formulation. The ethyl acetate extract was found to have good antioxidant activity. For HPLC fingerprint profiling a RP18 column was used with 80 % methanol as mobile phase.

Abstract No. C-293, 61st IPC (2009). HPTLC of gallic acid in fruits of Phyllanthus emblica on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. The hRf value of gallic acid was 40. Quantitative absorbance measurement at 280 nm. The method was linear in the range of 40-240 ng/band. The method was reproducible and suitable for quality control.

Ind. J. Pharma. Sci. 72(1), 39-45 (2010). The Ayurvedic Pharmacopoeia of India recognized roots of the three plants Cissampelos pareira, Cyclea peltata, and Stephania japonica (all Menispermaceae) as source for the marketed drug Patha. HPTLC fingerprint analysis of methanolic extracts of roots of all three plants on silica gel with n-butanol - ethyl acetate - formic acid - water 3:5:1:1. Detection under UV 365 nm (crude extract) and 295 nm (total alkaloids). Quantification of the marker berberine by HPLC. The three plants exhibit significantly different physico-chemical features and chromatographic fingerprints. Roots of C. pareira contained the highest concentration of berberine, S. Japonica contained very low amounts and C. peltata no berberine at all.

Modern J. of Integrated Trad. Chinese & Western Med. 19(31), 3439-3441 (2010). TLC on silica gel with chloroform – methanol – water 13:7:2. Detection by spraying with 10 % slfuric acid in ethanol and heating at 105 °C until the zones are visualized, evaluation under UV 366 nm. Identification of the component drugs Radix Astragali and Rhizoma Chuanxiong P.E by comparison of the retention values and color of the zones by the active compounds astragaloside and ferulic acid in the individual drug.

J. of Qilu Med. & Pharm. 29(11), 658-659 (2010). TLC on silica gel with 1) benzene – methanol 27:1; 2) toluene – methanol 17:1 ; 3) cyclohexane – propanone 10:3:4) petroleum ether (60-90 ºC) – ethyl acetate – formic acid 85:15:2, or 80:20:1. Detection by spraying with 1 % vanillin in sulfuric acid and heating at 100 °C until the zones were visualized. Identification by comparison of the fingerprint with the characteristic reference standards magnolol and honokiol. System 4) provided the best separation.

J. Planar Chromatogr. 24, 394-399 (2011). TLC of leave extracts and six flavonoids as markers (vitexin, isovitexin, orientin, isoorientin, quercetin, and tricin) on silica gel, prewashed with methanol and methylene chloride, with methanol - ethyl acetate - acetone - methylene chloride in different ratios using automated multiple development. The developed plate was dried in air for 2 h and sprayed with 1 % aluminum trichloride in ethanol. Then the plate was left for 2 h for derivatization in a glass drying chamber. Quantitative determination by densitometry at 366 nm. The hRf values of the six marker flavonoids were 22, 31, 38, 45, 57, and 88, respectively. Linearity was between 175 and 1750 ng/band. Instrument precision (n = 10) was between 0.2-0.9 %. The repeatability for standards and samples (n = 9), was 0.7 and 0.5, 0.8 and 0.5, 0.8 and 0.5, 0.8 and 0.4, 1.3 and 0.7, 1.1 and 0.3 % for isoorientin, orientin, isovitexin, vitexin, quercetin, and tricin, respectively. The limits of detection were 35, 40, 35, 50, 80, and 20 ng/zone for isoorientin, orientin, isovitexin, vitexin, quercetin, and tricin, respectively. The intra-day and inter-day precision was between 0.1-2.9 % and 0.3-2.4 % for all six marker flavonoids.

J. Planar Chromatogr. 21, 431-436 (2008). HPTLC of sponge extracts (dienon, aeroplysinin-1, avarone and avarol as standards) on silica gel, prewashed with methanol, by automated multiple development in the AMD2 with a fifteen-step gradient based on methanol, dichloromethane, and n-hexane. Optional derivatization was performed by dipping (speed 4 cm/s, immersion time 1 s) the plate into sulfuric acid reagent followed by heating for 5 min at 110 °C. For bioluminescence detection the developed plate was dipped (speed 3.5 cm/s, immersion time 0 s) into a luminescent Vibrio fisheri bacteria suspension. Detection of bioluminescence with the Bioluminizer. Identification of zones of interest by MS. Visual LOD of avarol and avarone was 70 and 60 ng/band, respectively.

J. Planar Chromatogr. 24, 497-502 (2011). HPTLC of isoswertisin-5-O-beta-D-glucoside (1), swertiamarin (2), and swertisin (3) as biomarkers on silica gel with ethyl acetate - methanol - water 16:2:1 in a twin-trough chamber with saturation for 30 min. Quantitative determination by absorbance measurement at 287 nm. Linearity was between 25-75 µg/mL for (1), 200-600 µg/mL for (2), and 100-300 µg/mL for (3). The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was below 2 %. The average recovery was 99.9 % for (1), 99.6 % for (2), and 99.1 % for (3). The hRf values were 32 for (1), 41 for (2), and 52 for (3). The limit of detection was 570 ng, 740 ng, and 300 ng for (1), (2), and (3), respectively.