J. Chromatogr. A 1490, 201-211 (2017). HPTLC for comparison of the phenolic profiles of polar extracts from Populus nigra L. (1), Populus alba L. (2) and Salix alba L. (3) buds. Five chemotypical patterns were distinguished after derivatization with Natural Products reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, which directly linked to active molecules in the chromatograms. The results showed that polyvalent compounds were evident when all derivatization and activity assays were combined together. Detection of at least three antimicrobial compound zones using Aliivibrio fischeri and Bacillus subtilis bioassays and of one phyto-estrogen with the planar yeast estrogen screen in Populus buds. Detection of several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase in all samples. Bioactive compounds were assigned by HPTLC-MS, e.g. chrysin as selective cholinesterase inhibitor, and caffeic acid and galangin as antimicrobials in (1) and (2). The method is suitable to determine the botanical origin and quality of Populus bud extracts and propolis samples.

Synthesis of oxidative degradation products from the methylated component. Phenols of anacardium occidentale and other phenolic lipids: confirmation of the structure of the parent phenols and of a related material. Lipids 17, 561-569 (1982). Isolation of the degradation products of anacardic acid, cardol, 2-methyl-cardol and cardanol by TLC on silica with a) chloroform, b) chloroform ethyl acetate 95:5, c) chloroform - petrol ether 6:4. Detection and visualization acc. to J. Caplin and J. Tyman, J. Chem. Res. 5, 34-35, 11, 0321- 0351 (1982).

Shanghai 1st. Med. Coll.(Shanghai Diyi Yixueyuan Xuebao) 11, 284-287 (1984). (Chinese). (Evaluation of total alkaloids in Chinese traditional drugs fen fang ji by TLC-spectrophotometric method.) TLC of tetradrine on silica with methanol - chloroform 1:5. Detection by UV. Quantification by spectrophotometry of methanol - eluate at 282 nm.

18.) Quantitative analysis of sennosides A and B in senna cultivated in Japan by thin-layer chromatography/densitometry method.) TLC of sennoside A and B on silica with iso-propanol - ethyl acetate - water - formic acid 17:19:12:2. Quantification by densitometry at 350 nm.

Phytochemistry 25, 2551-2553 (1986). TLC of ten flavonoid aglycones on polyamide with toluene - cyclohexane - methanol - MEK 12:6:1:2 or ethylformate - cyclohexane - butylacetate - formic acid 50:25:23:2. Detection by UV. Final purification of flavonoids with highest Rf values by PLC on polyamide with cyclohexane - toluene - MEK 12:1:1.

(Tea drugs. Improved release of active ingredients via pressure treatment and fast release PEX method.) TLC of anthraquinone glycosides on silica with petrol ether - ethyl acetate - formic acid 75.24:1. Densitometry by absorbance at 430 nm. Determination of glycosides and aglycones separately.

(Chinese). J. Chinese Herb Med. 19, 93 (1988) (Zhong Caoyao). TLC on silica with butanol - ethyl acetate - water 4:1:5. Detection by spraying with sulfuric acid - methanol 1:1 and heating at 105 °C. Comparison of the chromatograms under UV at 365 nm.

(Chinese). J. Chinese Herb Med. 19, 66-68 (1988) (Zhongcaoyao). TLC on silica with chloroform - ether 2:1. Detection by spraying with 2% 3,5-dinitrobenzoic acid and 5% NaOH in ethanol. Quantification by densitometry at 218 nm. Recovery > 96%. Calibration curve was linear over range at 0.2-1.0 µg.