J. Chem. Pharm. Res. 2(1), 155-161 (2010). A chroman derivative (C16O4H22) was isolated from the ethanolic extract of dried seeds of Ensete superbum. HPTLC on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The linear range was 300-900 ng/band. The amount of the chroman in different fractions of the extract was 1.83 % (ethanol fraction), 1.74 % (ethyl acetate fraction) and 0.74 % (methanol fraction).

J. of Guangzhou Chem. Engin. 39(1), 144-145 (2011). A course on the technology of TLC/bioautography applied for the analysis of TCM was set-up to enhance students’ understanding of theoretical knowledge and to train and improve the interest and skill of students in chemical experiments. Demonstration of the TLC analysis of rutin and quercetin in Flos Sophorae on silica gel with ethyl acetate – formic acid – water 8:1:1. Detection under UV 254 nm and 366 nm, and by immersing into a solution of 1,1-diphenyl-2-picrylhydrazyl in ethanol (DPPH radical reagent). The result of this practice was satisfactory, and the course proved to be a good example to utilize the modern technology in the experimental teaching.

J. Planar Chromatogr. 23, 323-325 (2010). HPTLC of taraxerol on silica gel with hexane - ethyl acetate 9:1 in a saturated twin-trough chamber. Detection by spraying with anisaldehyde - sulfuric acid reagent followed by heating for 5 min at 80 °C. Quantitative determination by absorbance measurement at 540 nm. The average recovery was between 99.6 and 100.3 % with %RSD less than 2 %. For both intra-day and inter-day precision %RSD was less than 2 %. The LOD and LOQ were 47 and 140 ng/band, respectively. The linearity range was 0.5-2.5 µg/band. The hRf value of taraxerol was 22.

J. Planar Chromatogr. 24, 121-124 (2011). HPTLC of kurarinone and sophoraflavanone G in the roots of Sophora flavescens on silica gel with chloroform - methanol 10:1 with chamber saturation for 30 min at 20 °C. Quantitative determination by absorbance measurement at 285 nm. The intra-day and inter-day %RSD were 1.9-2.0 % and 2.2-2.4 %, respectively. Instrument precision and repeatability of the method were 0.4-0.6 and 1.81-1.84 %, respectively. Average recovery was 96.3-103.4 % for kuraninone and 96.8-102.9 % for sophoraflavanone G. The limits of detection and quantification were 27 and 80 ng/band for kurarinone and 12 and 36 ng/band for sophoraflavanone G. Linearity was in the range of 200-1200 ng/band for kurarinone and 90-550 ng/band for sophoraflavanone. The hRf value was 31 and 50 for kurarinone and sophoraflavanone, respectively.

Chinese J. of Ethnopharm. 2, 57-59 (2011). TLC of the extracts of Xinnao Tongtai capsules 1) for Lobed Kudzuvine Root, on silica gel with chloroform - methanol - water 28:10:1, detection under UV 366 nm; 2) for Szechwan Lovage Rhizome and Angelica sinensis, on silica gel with n-hexane - ethyl acetate 9:1, detection under UV 366 nm. Quantification of gastrodin by HPLC. The procedures proved to be simple, accurate, reproducible, robust and suitable for the quality control of the medicine.

J. Planar Chromatogr. 24, 306-311 (2011). HPTLC of ethanolic leaf extracts of A. squamosa, with rutin and isoquercitrin as standards, on silica gel (prewashed with methanol) with ethyl acetate - formic acid - glacial acetic acid - ethyl methyl ketone - water 50:7:3:30:10 with chamber saturation for 30 min at room temperature (25 +/- 2°C) and a relative humidity of 50 +/- 5 %. Quantitative determination by densitometry at 366 nm. Linearity was between 200-1600 ng/band for both rutin and isoquercitrin. The robustness of the method (%RSD) was 1.9-2.9 % for rutin and 1.5-2.3 % for isoquercitrin, respectively. The instrumental precision (%RSD) was 0.9 and 0.3 % for rutin and isoquercitrin, respectively. The intra-day and inter-day precision (%RSD) was less than 3 % in all cases. LOD and LOQ was 75 and 100 ng/band for rutin and 40 and 80 ng/band for isoquercitrin, respectively. The hRf value was 32 for rutin and 58 for isoquercitrin. Recovery (by standard addition) was found to be 96-107 %.

PHCOGJ 2(8), 211-215 (2011). TLC finger-print profiling of 4 cinnamomum species, C. malabatrum, C. sulphuratum, C. tamala and C. zeylanicum has been reported. The hexane extracts of the plants were separated on silica gel with toluene - ethyl acetate 8:1. Densitometric evaluation at 254 nm. Detection by dipping in vanillin-sulfuric acid reagent followed by heating at 105 °C and evaluation at 620 nm. The hRf value of eugenol was 69. The content of eugenol were higher in C. zeylanicum than in the other species. The fingerprint profile showed some other bands.

J. of China Pharm. 21 (20), 34-35 (2012). Zhongtongxiao capsules are a herbal TCM preparation to treat blood stasis and sore swellings caused by fracture. TLC of the extracts of the medicine on silica gel 1) for Angelica sinensis and the rhizome of Chuanxiong, with n-hexane – ethyl acetate 9:1, detection at UV 366 nm; 2) for Radix Paeoniae, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and heating at 105 °C until the zones are visible, viewing in daylight; 3) for Teasel, developed with toluene – ethanol 9:2, detection by spraying firstly with potassium iodobismuthate – hydrochloric acid – water 10:1:200 and then with 5 % nitrous acid in ethanol, followed by viewing in daylight; 4) for Licorice, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, viewing in daylight.