J. Sep. Sci. 34, 749-760 (2011). HPTLC of (-)-epicatechin (1), (-)-epicatechin gallate (2), (-)-epigallocatechin gallate (3), caffeine (4), rutin (5), quercetin (6), gallic acid (7), ellagic acid (8), caffeic acid (9), and ferulic acid (10) in the leaves of green tea (Camellia sinensis) and guava (Psidium guajava) on silica gel with toluene – acetone – formic acid 5:4:1 for compounds (1) - (6) and toluene – ethyl acetate – formic acid – methanol 15:15:4:1 for compounds (7) - (10). Quantitative determination by absorbance measurement at 282 nm for compounds (1) - (6) and 285 nm for compounds (7) - (10). The hRf values of compounds (1) - (10) were 49, 37, 26, 60, 8, 66, 49, 34,62 and 70, respectively. Linearity was between 100-350 ng/band for compounds (1) - (5), 66.6-233.2 ng/band for compound (6) and between 50-300 ng/band for compounds (7) - (10). The limits of detection were found to be 60 ng/band for compounds (1) - (3), 30 ng for compounds (4), (5) and (8), 40 ng/band for compound (6), 20 ng/band for compound (7) and 10 ng/band for compounds (9) and (10). The limits of quantification were 100 ng/band for compounds (1) - (3), 60 ng/band for compounds (4) - (7), 30 ng/band for compounds (9) - (10), and 75 ng/band for compound (8). Inter- and intraday precisions were below 1.50 % and 2.84 %, respectively. Recoveries were found in the range of 95-100 %.

Chinese J. Ethnomed. Ethnopharm. (23), 61-64 (2010). Optimization of the sample preparation procedure for Herba Saururi chinensis 1) by ultrasonication with methanol for 60 min; 2) by ultrasonication with methanol for 20 min and filtration through neutral alumina column with methanol; 3) by reflux extraction with 80 % methanol for 60 min and extraction with diethyl ether; 4) by ultrasonication with ethanol for 60 min; 5) by ultrasonication with methanol - 25 % hydrochloric acid 4:1 for 60 min and extraction with ethyl acetate. Procedure 5) was best suited. TLC on silica gel 1) with petroleum ether (60-90 °C) – acetone 5:2, and detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones appear; 2) with toluene – ethyl acetate – formic acid 5:2:1, and detection by spraying with 1 % AlCl3 in ethanol and evaluation under UV 366 nm; 3) with n-hexane – ethyl acetate – formic acid 70:50:8, and detection by spraying with 1 % AlCl3 in ethanol, heating at 105 ºC and evaluation under daylight or under UV 366 nm; 4) with toluene – ethyl acetate – formic acid 5:4:1 with chamber saturation with hydrochloric acid vapor, detection under daylight or UV 366 nm.

Planta Med. 75, 1000 (2009). TLC and HPTLC of Mezereon (Daphne mezereum) bark extracts and scopoletin, umbelliferone, mezerein, and daphnetoxin on silica gel with various mobile phases containing toluene, ethyl acetate, and formic acid at different proportions. Detection under UV 254 and 366 nm and visible light. The applied procedure may be proposed for an updated and optimized TLC identification test of the homeopathic monograph of Daphne mezereum L.

Ham. Ex D. Don, Myricaceae. J. Planar Chromatogr. 23, 326-331 (2010). HPTLC of myrecitin and stem bark extracts on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:3:2. Quantitative determination by absorbance measurement at 268 nm. Linearity was between 0.4-2.0 µg/band. The limits of detection and quantitation were 93 ng and 284 ng/zone. The intra-day and inter-day precision of the method was in the range of 0.14-0.55 %. The recovery of myrecitin at three concentrations was in the range of 98.9-100.1 % and the average recovery was 99.3 %.

Chinese J. of Pharm. Anal. 29 (12), 2168-2179 (2009). TLC of the extracts of 11 varieties of herbal drugs and preparations on silica gel (conditioned with 1 % iodine in dichloromethane) with cyclohexane - dichloromethane - ethyl acetate - glacial acetic acid 200:50:80:1. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C. Identification of oleanolic acid and ursolic acid by comparison of the hRf values (hRf 54 for oleanolic acid and 38 for ursolic acid, respectively) with standards.

Planta Med. 75, 976 (2009). TLC of rosmarinic acid and rutin in whole plant powder of Hymenocrater bituminosus Fisch. and C. A. Mey. on silica gel with ethyl acetate - methanol - water - formic acid 72:8:13:7 for rutin and acetone - toluene - formic acid 4:5:1 for rosmarinic acid. Quantitative determination by densitometry in absorbance mode at 366 nm. The linear range for rutin was 50-450 ng/zone and for rosmarinic acid 150-600 ng/zone, both with a correlation coefficient of 0.991.

Journal of Pharmacy Research 4(5), 1353-1355 (2011). HPTLC of methanolic extracts of Aegle marmelos fruit pulp on silica gel with toluene - ethyl acetate - glacial acetic acid 70:30:1. Densitometric quantification of marmelosin at 310 nm. The method was linear in the range of 50-350 ng/band. The method was used to determine the marmelosin content of pulp, unripe pulp, seeds, leaves, rind, outer stain and inner stain.

Chinese J. Prac. Med. 7 (3), 251-252 (2012). Presentation of a procedure for preparation and quality control of Qichai Hugan pills. TLC of the extracts 1) for Astragalus mongholicus, on silica gel with the lower phase of chloroform - ethyl acetate - methanol - water 10:20:11:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min and viewing under daylight and UV 366 nm; 2) for the root of Chinese thorowax, on silica gel with the lower phase of chloroform - methanol - water 7:3:1, detection by spraying with a solution of p-dimethylaminobenzaldehyde - sulfuric acid - ethanol 1:2:18 followed by heating at 105 °C and viewing under daylight; 3) for the fruit of Chinese wolfberry, on silica gel with toluene – ethyl acetate - formic acid 15:5:2, detection under UV 366 nm; 4) for the root of red-rooted salvia, on silica gel with chloroform - acetone - formic acid 60:5:2, detection by spraying with a solution of 2 % ferric chloride - 1 % potassium ferricyanide 1:1 and viewing under daylight.