Phytochem. Anal. 33, 83-93 (2022). HPTLC of table olives (Olea europaea) on silica gel with dichloromethane - methanol - acetic acid 45:5:1. Detection by spraying with sulfuric vanillin reagent (5 % vanillin in methanol - 5 % sulfuric acid in methanol 1:1) and under UV light at 254 and 366 nm. Further analysis by nuclear magnetic resonance. 

Phytochem. Anal. 33, 115-126 (2022). HPTLC bioautography of 14 Egyptian plants on silica gel with methylene chloride - methanol 9:1 (system I) or ethyl acetate - methanol - water - glacial acetic acid 120:20:16:1 (system II). Detection by spraying with anisaldehyde/sulfuric acid reagent. Aromatase inhibitory assay was performed by dipping into 112.5 mL cofactor solution (containing 4.5 mL of 0.5 % sodium phosphate buffer, 4.5 mL NADPH regenerating system solution A and 0.9 mL NADPH regenerating system solution B and then completed to volume with distilled water), followed by incubation at 37 °C for half an hour, then re-immersed in 75 mL aromatase E/S mix (containing 120 μL aromatase enzyme, 0.03 mL DBF and 3 mL albumin dissolved in 0.075 M potassium phosphate buffer) and re-incubated for another half an hour. Enzymatic reaction was terminated by immersing plates into 2N sodium hydroxide stop solution. Active zones were observed under UV 366 nm. Calculation of the inhibition zones was performed by reciprocal iso-inhibition volume (RIV) that is based on measuring the zone pixel intensity. The hRF values for chrysin were 63 and 91 in systems I and II, respectivley. The intermediate precision was below 3 % (n=3). Linearity was between 0.1 and 0.3 μg/zone. LOD and LOQ were 80 and 206 ng/zone, respectively. Recovery was between 92.5 and 106.5 %. Two quantification methods, the peak area and RIV method were compared and the RIV method showed superiority over the peak area method with %RSD values of 0.98 and 1.49 compared with 2.86 and 3.58, respectively.

Chinese J. Ethnomed. & Ethnopharm. (10), 36-39 (2021). Huangjia Ruangan granules is a TCM preparation, which relaxes the liver and promotes blood circulation, and is used for treating liver fibrosis and early cirrhosis, spleen deficiency, liver depression and blood stasis. (A) Identification of Salvia miltiorrhiza Bge, Pueraria lobata (Willd.) Ohwi, and Paeoniae rubra root, by TLC of the methanol extracts, the control solutions lacking the three drugs, and the standards tanshinone IIA, puerarin, paeoniflorin, on silica gel first with chloroform – methanol – water 70:25:2.5 to 5 cm and then with toluene – ethyl acetate 24:1 to 8 cm; detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 ˚C until the zones are visible in white light and UV 365 nm. (B) Identification of Astragali Radix, Panax notoginseng (Burkill) F. H. Chen ex C. H.) and Radix Bupleuri, by TLC of the methanol extracts, the control solutions lacking the three drugs and the standards astragaloside lV, notoginsenoside R1 and saikosaponin A, on silica gel with butanol – ethyl acetate – water 4:3:5 to 17 cm, detection by spraying with 2 % ethanol p-dimethylaminobenzaldehyde solution - sulfuric acid 2:3 and heating at 80 ˚C until the zones are visible, evaluation in white light and at UV 365 nm. The method was successfully applyed to identify six major ingredients in Huangjia Ruangan granules on the same plate through multi-information obtained, and proved to be simple, fast, specific, reproducible, robust and well suitable for the purpose.

J. Trad. Chinese Veterinary Med. 55 (1), 15-19 (2021). Qixingcha Keli is a TCM preparation converted from human to animal use, for the treatment of gastric stagnation in piglets and other animals. Quality control of its component (A) Crataegus L. by TLC of the ethanol extracts, the control solutions with and without Crataegus L. on silica gel with ethyl ether - chloroform - formic acid 5:5:1 to 9 cm. Detection by heating the plates at 105 ˚C for 15 min, then spraying with 0.05 % bromophenol solution (pH = 7.5) and viewing in white light. For component (B) Glycyrrhiza uralensis Fisch., TLC of the ethanol extracts, the control solutions with or without Glycyrrhiza uralensis Fisch. on silica gel containing 1 % NaOH with ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:1 to 18 cm, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ˚C, evaluation in white light and at UV 365 nm. For (C) Uncaria rhynchophylla (Miq.) Miq. ex Havil., TLC of the ethanol extracts and the control solutions on silica gel with petroleum ether (60-90 ˚C) - propanone 3:2 to 9 cm, detection at UV 254 nm. For (D) Semen Coicis, by TLC of the ethyl acetate extracts and control solutions on silica gel with petroleum ether (60-90 ˚C) - ethyl ether - glacial acetic acid 166:34:1 to 9 cm, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:200 and heating at 105 ˚C, detection in white light and at UV 365 nm.

J. Food Compos. Anal. 93, 103616 (2020). HPTLC profile of a selected herbal dietary supplement containing seven herbal components as ingredients on the product label (capsicum, cactus, wheat, white bean, Garcinia cambogia, psyllium husk and black pepper) on silica gel with hexane - ethyl acetate 2:1. Detection by spraying with Dragendorff’s reagent or 10 % potassium hydroxide solution in ethanol for the detection of alkaloids and anthraquinones, respectively. Qualitative analysis under UV light at 254 and 366 nm. Further analysis by mass spectrometry. The method allowed the identification of contaminant species, including the hidden oleamide compound within the selected herbal product.

J. Liq. Chromatogr. Relat. Technol. 44, 87-94 (2021). HPTLC fingerprinting of Selinum
tenuifolium on silica gel with toluene - ethyl acetate - formic acid 13:11:2. Detection under UV light at 254 nm and 366 nm. The hRF values for gallic acid and ferulic acid were 36 and 58, respectively. 

J. Planar Chromatogr. 34, 89-94 (2021). HPTLC of fresh Kakadu plum fruits (Terminalia ferdinandiana) on silica gel with toluene - ethyl acetate - formic acid 5:3:2. Detection by dipping into natural product reagent (1.0 g of 2-aminoethyl diphenylborinate in 100 mL methanol), followed by heating at 100 ºC for 3 min. Quantitative determination by absorbance measurement at 366 nm. The HPTLC fingerprint allowed to differentiate between different sources of fruit.

Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).