J. of China Pharm. 22 (3), 13-15 (2013). Gushangling Jiaonang capsule is a herbal TCM for post traumatic functional recovery, prescribed to treat muscle atrophy, bone hyperplasia, neck and lumbar diseases, nerve injuries etc. For quality control, HPTLC on silica gel 1) for Paeonia lactiflora Pall., with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 3 % vanillin in sulfuric acid – water 1:200 and heating mildly until the zones are visible in daylight; 2) for Angelica sinensis, with n-hexane – ethyl acetate – 9:1, detection under UV 366 nm; 3) Astragalus membranaceus (Fisch.) Bunge., with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.

Mod. Chinese Med. 3, 232-236 (2014). Shuanghuang Tongfeng Jiaonang capsules are a TCM preparation for the treatment of hyperuricemia. For quality control, TLC on silica gel (1) for Paeonia veitchii and the standard paeoniflorin, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible under white light; (2) for Astragalus membranaceus (Fisch.) Bunge. and the standard astragaloside A, with the lower phase of chloroform – methanol – water 13:6:2 after placing at a temperature lower than 10 °C for a night, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible under white light; (3) for Cortex Fraxini and the standards esculin hydrate and esculetin, with chloroform – methanol – formic acid 20:5:1, detection at UV 366 nm. Quantification of astragaloside A by HPLC.

salina, and NMR profiles of extracts of lichens collected from Brazil and Antarctica. Quim. Nova. 37, 1015-1021 (2014). TLC of (1) atranorin, (2) salazinic, (3) barbatic, (4) alpha-alectoronic, (5) alpha-collatolic, (6) cryptochlorophaeic, (7) caperatic, (8) lobaric and (9) protolichesterinic acids in six lichen species named Parmotrema cetratum (Ach.) Hale (I), Parmotrema wainioi (A. L.Smith) Hale (II), Canoparmelia cryptochlorophaea (Hale) (III), Parmotrema mesotropum (Müll. Arg.) Hale (IV), Cladia aggregata (Sw.) Nyl. (V) and Stereocaulon alpinum Laurer (VI), on silica gel with toluene - ethyl acetate - acetic acid 6:4:1 for (I, IV and V) and toluene - acetic acid 17:3 for (II, III and VI). Detection by spraying with p-anisaldehyde - sulfuric acid, followed by heating at 110 ºC. The hRF values of (1) and (2) in (I) were 73 and 33, respectively, whereas the value of (3) and (4) in (II) were 15 and 36; the value of (1) in (III) was 71. DPPH radical scavenging activity was also investigated.

Journal of Pharmacognosy and Phytochemistry 4 (2), 1-4 (2015). HPTLC of petroleum ether and ethanol extracts of aerial parts of Blumea lacera D.C. (Asteraceae), collected in the Gorakhpur district, with chloroform - benzene - formic acid 15:5:3. Evaluation in daylight revealed 11 zones, at least 9 of them at hRf between 3 and 47. The ethanol extract was purified by washing with water, chloroform and ethyl acetate successively, and then by fractionation on a silica gel (60-120 mesh) column; the benzene subfraction yielded, after exposure to iodine vapor, one yellow zone (hRf 41), identified (by IR, NMR and MS) as 3,5-dihydroxy-3’-methyl-6,7,4’-trimethoxyflavanone.

J. Ethnopharmacol. 181, 236-251 (2016). Analysis of the botanical description, geographical location, traditional and medicinal uses, phytochemistry and pharmacological investigation, toxicological aspects, patent information and clinical studies of Symplocos racemosa. The review described methods for the determination of loturine in the bark extracts of Symplocos racemosa on silica gel with chloroform – acetonitrile – triethylamine 7:5:2, quantitative determination by absorbance measurement at 280 nm.

CBS 114, 10-12 (2014). HPTLC of plant extracts on silica gel with either (1) dichloromethane – methanol – water 70:30:4 for polar extracts, (2) ethyl acetate – methanol – water – formic acid 50:10:7:1 for methanol extracts containing very polar compounds, or (3) toluene – ethyl acetate – formic acid 40:10:1 for lipophilic extracts. Detection by bioautography: Spraying with 2.5 mL substrate solution (0.047 g of L-DOPA mixed with 20 mL of phosphate buffer containing 1 % Triton X-100) and 3 mL of enzyme solution (mushroom tyrosinase in phosphate buffer, activity 400 U/mL), followed by incubation for 10 min at room temperature. Detection under UV 254 nm and 366 nm and white light. Positive results appeared as white inhibition zones on the dark plate background.

Phytochem. Anal. 27, 239-248 (2016). HPTLC of Echium vulgare from different sites (characterized by varied heavy metal contamination in the substrates) on silica gel with ethyl acetate – 98-100 % formic acid – glacial acetic acid – water 100:11:11:26. Qualitative identification under UV light at 366 nm. Pre-processing of TLC plate images was performed using open architecture software.

Planta Medica 82(18), 1546-1552 (2016). The fractionation of the butanol-soluble part of the methanol extract of Lysimachia ciliata was monitored through TLC on silica gel with chloroform – methanol – water 23:12:2, detection by spraying with sulfuric acid. The saponin-containing fractions were mixed and submitted to preparative TLC on silica gel (same mobile phase). Saponins, eluted with methanol from the water-sprayed layer, were analyzed by UPLC-ESI-MS/MS; two were identified as desglucoanagalloside B and anagallosaponin IV (29.1 % and 8.2 % of the saponin fraction, respectively).