J. AOAC Int. 88, 1568-1570 (2005). HPTLC of alliin on silica gel with n-butanol - acetic acid - water 3:1:1 at 25 +/- 2 °C and 40 % relative humidity. Detection by dipping in ninhydrin reagent (0.3 g ninhydrin in 100 mL n-butanol and 3 mL acetic acid) for 2 s, followed by heating at 110 °C for 5 min. Quantitative determination by densitometric evaluation of peak areas at 540 nm. Linearity within the range of 250-1500 ng/spot, correlation coefficient of 0.998 and RSD of 2.87 %; mean recovery 98.4 %.

Planta Med. 72, 351-357 (2006). Analytical and preparative TLC of ergosta-4,6,8(14),22-tetraen-3-one, beta-sitosterone, 2,6,11-trimethyldodeca-2,6-10triene, and stigma-4-ene-3,6-dione on silica gel with n-hexane - ethyl acetate 6:1. Preparative TLC of tsangin A with dichloromethane - acetone 25:1; of tsangin B and beilschmien C with dichloromethane - acetone 20:1; of beilschmien A and B with n-hexane - ethylacetate 2:1. Detection under UV light at 254 nm.

Planta Med. 70, 585-588 (2004). Preparative TLC of rotenon and 6a-alpha,12a-alpha-12a-hydroxyelliptone on silica gel with dichloromethane, benzene - methanol 24:1 and hexane - ethyl acetate 4:1. Detection under UV light at 254 nm.

Chromatographia 63 (3-4), 209-213 (2006). HPTLC of valerenic acid in Valeriana jatamansi and Valeriana officinalis on silica gel with hexane - ethyl acetate - acetic acid 160:40:1. Detection with anisaldehyde-sulphuric acid reagent. Quantitative determination by absorbance measurement at 700 nm. The calibration curves were linear in the range of 500 ng - 2.5 µg/zone.

Phytochem. Anal. 17, 394-397 (2006). HPTLC of phyllanthin and hypophyllanthin from Phyllantus species, e.g. Phyllantus amarus, on silica gel with hexane – acetone – ethyl acetate 37:6:4. Detection by spraying with vanillin in concentrated sulfuric acid and ethanol. Quantitative determination by absorbance measurement at 580 nm. Recovery of phyllanthin is 98.7 % and of hypophyllanthin 97.3 %.The method was validated and the peak purities and limits of detection and quantification were determined.

Pharmazie 60, 455-457 (2005). Preparative TLC of nervogenic acid, nervogenic acid methyl ether, 2,2-dimethyl-8-(3-methyl-2-butenyl)-2H-chromene-6-carboxylic acid, dillapional, dillapiol aldehyde, N-trans-feruloyltyramine, omega-hydroxyisodillapiole, 4-hydroxy-3-(3-methyl-2-butenyl)benzoate, and three new 4-hydroxy-benzoic acid derivatives, 4-methoxy-3,5-bis-(3-hydroxy-3-methyl-1-butenyl)benzoate, 3-hydroxy-2-(1-hydroxy-1-methylethyl)-2,3-dihydroxybenzofuran-5-carboxylic acid, and 3-hydroxy-2-(1-hydroxy-1-methylethyl)-2,3-dihydrobenzofuran-5-carboxylic acid methyl ester on silica gel with chloroform - ethyl acetate - formic acid - 90:10:1; chloroform - methanol 9:1; 8:2; and ethyl acetate - formic acid - water 82:9:9. Detection under UV light.

Pharmazie 61, 670-672 (2006). TLC of swertisin and 2’’-O-rhamnosylswertisin on silica gel with chloroform - methanol 7:3 and 17:3. Detection under UV light at 254 nm or by spraying with 2 % iron(III) chloride solution in ethanol. The compounds were identified by direct comparison with authentic samples.

J. Sep. Sci. 30, 2092-2096 (2007). HPTLC of flavonoids in Bauhinia variegata, Bacopa monnieri, Centella asiatica, Ginkgo biloba, Lonicera japonica, Rosa bourboniana, Rosa brunonii, and Rosa damascena on RP-18 with two-fold development with water (5 % formic acid) – methanol 7:3 and water (5 % formic acid) – methanol 1:1 as mobile phases. Quantitative determination by absorbance measurement at 280 nm. The hRf values of apigenin (1), quercetin (2), rutin (3), luteolin (4), and quercitrin (5) were 19, 29, 34, 51, and 63, respectively. Linearity was between 150 and 800 ng/zone for (1) and (3) and between 200 and 1000 ng/zone for (2), (4) and (5). The limits of detection and quantification for (1) – (5) were 30 and 166 ng/zone, 40 and 200 ng/zone, 20 and 150 ng/zone, 40 and 200 ng/zone, and 40 and 200 ng/zone, respectively. Recovery was between 97 and 99.8% for (1) – (5).