J. AOAC Int. 92, 785-788 (2009). HPTLC of plant extracts and herbal preparations applied bandwise on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:25 after preconditioning for 5 min. Detection by immersion in natural products reagent (diphenylboric acid 2-aminoethylester) followed by polyethylene glycol 400 reagent for 2 s. After air-drying the plates were evaluated under UV 366 nm.

Abstract No. 9286, IHCB (2009). HPTLC of dially disulfide in garlic oil macerate on silica gel with n-hexane - isopropyl alcohol - formic acid 196:4:3. Quantitative determination by absorbance measurement at 210 nm. The method was linear in the range of 16-48 µg/spot.

J. Planar Chromatogr. 22, 381-384 (2009). TLC of aloin in commercial Ayurvedic preparations on silica gel (prewashed with methanol) with ethyl acetate - methanol - water 50:7:2 in a twin trough chamber with chamber saturation for 5-7 min at 30 +/- 4 °C and a relative humidity of 57 +/- 3 %. Quantitative determination by absorbance measurement at 360 nm. The limit of detection and quantification was 10 and 20 ng/band, respectively.

J. Planar Chromatogr. 22, 293-296 (2009). TLC of verbascoside, forsythoside B, caffeoyl-malic acid and plant (Ballota nigra, B. hirsuta, and B. rupestris) extracts on silica gel with formic acid - acetic acid - water - ethyl acetate 15:15:36:134. Quantitative determination by fluorescence measurement at 395 nm. It was observed that amounts of phenylpropanoids in Ballota nigra leaves increase during the main and secondary flowering periods in June.

Abstract No. C-495, 61st IPC (2009). Screening of different phytoconstituents in a polyherbal tablet formulation. TLC of n-hexane, chloroform and methanol extracts of the tablets on silica gel with n-hexane - ethyl acetate 7:3; chloroform-methanol 9:1, and chloroform - glacial acetic acid - methanol - water 8:40:15:10. Evaluation under UV 254 nm as well as under UV 366 nm after spraying with different reagents: 20 % sulfuric acid, aniline-hydrogen phthalate reagent, anisaldehyde-sulfuric acid reagent, and vanillin-sulfuric acid reagent for the detection of piperine and andrographide, the active constituents present in formulations like Tefroliv Forte tablets. Other constituents (tannins etc) were analyzed by UV spectrophotometry.

Abstract No. C-97, 61st IPC (2009). Chromatographic methods are reported for identification (TLC) and quantification (HPTLC) of withaferin-A in methanolic and chloroform extract of dried fruits of Withania coagulans. Chromatographic separation on silica gel with toluene - ethyl acetate - formic acid 5:5:1. The identification of withaferin-A in both chloroform and methanolic extracts was performed by comparison of hRf values and UV absorbance maxima (209 nm). Quantification was performed by absorbance measurement at 540 nm after spraying the developed plate with Liebermann-Burchard reagent. Methanolic extracts and chloroform extracts contained 3.67 mg/g and 2.10 mg/g of withaferin-A, respectively. No withaferin-A was found in hydroalcoholic extracts.

Phytochem. Anal. 22, 59-65 (2011). TLC and free radical scavenging fingerprints of nineteen Salvia species on silica gel with toluene - ethyl acetate - formic acid 60:40:1 for the less polar constituents and ethyl acetate - water - formic acid - acetic acid 100:26:11:11 for the medium and highly polar substances. After drying at room temperature for 15 min derivatization with vanillin sulfuric acid reagent (1 g vanillin with 20 % sulfuric acid in methanol) followed by heating for 5 min at 105 °C. Quantitative determination by absorbance measurement at 254 nm. Free radical scavenging properties were investigated by spraying the plate with DPPH radical reagent (0.2 %) in methanol and left at ambient temperature for 30 min. The strongest free radical scavenging activity was observed for rosmarinic acid, with an hRf of 70.

Anal. Chim. Acta 690 (2), 148-161 (2011). Chromatographic fingerprinting has been generally accepted as analytical method for the quality control of herbal medicines. This review describes the evolution of the regulations and guidelines on the quality control of herbal medicines, and reviews the established analytical techniques in TLC, HPLC, UHPLC, hydrophilic interaction chromatography, and GC. Emphasis is put on the most recent developments, such as miniaturized techniques, new stationary phases, analysis at high temperatures and multi-dimensional chromatography. The new chemometric data handling techniques are discussed.