Planta Medica 82 (15), 1351-1358 (2016). The barks of forest trees (Acer pseudoplatanus, Alnus glutinosa, Fagus sylvatica, Fraxinus excelsior, Larix decidua, Picea abies, Populus robusta, Populus tremula, Prunus avium, Quercus robur) were successively extracted with n-heptane, methanol, and methanol/water; extracts and gentamycin were applied on TLC plates (but not developed; no TLC), which were sterilised, covered with a Mueller-Hinton agar medium containing a Staphylococcus aureus CIP 53.154 suspension, incubated at 37°C for 24 h, and revealed with MTT. All the methanolic extracts were active, as well as some other, the most active being those of Q. robur, L. decidua, and P. abies.

Chinese J. of Drug Evaluation 32 (4), 193-194 (2015). Fortune Paulownia leaf, the dry leaf of Paulownia fortunei (Seem.) Hemsl, is a TCM raw material used for treatment of carbuncles, furuncles, trauma, hemorrhage, etc. For quality control, TLC of its extracts on silica gel previously impregnated with 1 % iodine in dichloromethane and developed with cyclohexane – ethyl acetate – glacial acetic acid 20:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, viewing (A) in white light and (B) under UV 366 nm. Identification by fingerprint comparison with the standards oleanolic acid (hRf 67) and ursolic acid (hRf 60) and the reference raw material in parallel.

Chinese J. Hosp. Pharm. 35 (4), 288-292 (2015). Zhichuang Xunxi Ye fumigant is a herbal TCM for the treatment of hemorrhoids. For quality control, TLC of its extracts, (1) for Leatherleaf Mahonia Stem, on silica gel preconditioned with ammonia vapor and developed with toluene – ethyl acetate – methanol – isopropanol - ammonium hydroxide 12:6:3:3:1, detection at UV 366 nm; (2) for Radix sophorae flavescentis, on silica gel impregnated with 2 % NaOH, with (A) toluene – acetone – methanol 16:6:1, (B) the upper phase of toluene – ethyl acetate – methanol – water 2:4:2:1 under 10 ºC overnight, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and evaluation in daylight; (3) for Phellodendron Chinense Schneid., on silica gel with chloroform - methanol – water 30:15:4, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and evaluation in daylight. Quantification of berberine chloride hydrate and matrine by HPLC.

J. Chromatogr. A 1532, 198-207 (2018). Development of a method to analyze the efficiency of a diverse set of natural deep eutectic solvents (NADES) for the extraction of compounds of interest from two model plants, Ginkgo biloba and Panax ginseng. HPTLC on silica gel with toluene – ethyl acetate – acetone – methanol 50:25:25:3 for ginkgolides in Ginkgo biloba leaves; with ethyl acetate – acetic acid – formic acid – water 100:11:11:27 for phenolics in Ginkgo biloba leaves; with toluene – ethyl acetate – acetic acid 40:10:1 for ginkgolic acids in Ginkgo biloba leaves; with chloroform – ethyl acetate – methanol – water 15:40:22:10 for ginsenosides in Panax ginseng leaves and stems. The six different NADES were combinations of two or three compounds mixed in defined molar ratios, e.g. malic acid – choline chloride 1:1, malic acid – glucose 1:1, choline chloride – glucose 5:2, malic acid – proline 1:1, glucose – fructose – sucrose 1:1:1 and glycerol – proline – sucrose 9:4:1. Processing the data obtained by multivariate data analysis showed differences between the extracts. Discussion of the foreground of application of NADES in green chemistry and the advantages of NADES as green solvents used in novel green products for the food, cosmetics and pharmaceuticals.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 8 (1984). HPTLC of trigonelline on silica with propanol - methanol - 0.1 M CH3COONa in water 20:3:30. Detection with Dragendorff reagent and by UV. Fluorometry with excitation at 270 nm or, after Dragendorff staining, by absorbance at 525 nm.

Biochemical Systematics and Ecology 13, 169-1 70 (1985). Analytical and preparative TLC on silica developed five times in chloroform - methanol 9:1 or six times in chloroform - methanol 85:15 in a chamber saturated with NH3; authentic samples of aristolochic acids were used as standards.

Determination by densitometry at 270, 240 and 245 nm for the three substances. Rel. standard deviation in the range 0.8-1.7 %.

Detection by spraying with 10 % sulfuric acid and heating at 110 °C for 7 min. Quantification by densitometry at 520 nm.