J. Chinese Trad. Patent Med. 30 (11), 1642-1646 (2008). TLC of Yinao pill extracts on silica gel with 1) toluene - ethyl acetate 9:1; 2) benzene - ethyl acetate 3:2; 3) toluene - ethyl acetate - formic acid 28:8:1. Detection 1) by spraying with 10 % phosphomolybdic acid in ethanol and heating at 105 ºC; 2) by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC.

Abstract No. 9145, IHCB (2009). Terminalia chebula (an important constituent of Triphala, a popular ayurvedic formulation) was standardized for gallic acid content by HPTLC on silica gel with toluene - ethyl acetate - formic acid 10:10:3. The hRf value of gallic acid was 45. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 150-550 ng/spot. Hydroalcoholic extracts of the fruit pulp contained 10.5 mg/g gallic acid.

J. Chinese Trad. Patent Med., 30 (11), 1638-1642 (2008). TLC of Shujin Huoxue capsule extracts on silica gel with 1) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 2) cyclohexane - ethyl acetate 9:1; 3) toluene - acetone - ethanol - ammonia 16:12:1:4. Detection 1) by exposure to iodine vapor; 2) under UV 365 nm; 3) under UV 254 nm. Identification by comparison with the standards of the component drugs. Quantification of paeoniflorin by HPLC.

J. AOAC Int. 91, 1265-1270 (2008). TLC of aloin A and extracts of dried herbal products applied bandwise on silica gel with ethyl acetate - methanol - water 100:17:10 at 25 °C with chamber saturation. Detection by spraying with 1 % methanolic diphenylboric acid 2-aminoethylester (natural products reagent), followed by 5 % ethanolic polyethylene glycol 400 and visualization under UV light at 365 nm. The hRf value of aloin (as a mixture of aloin A and B stereoisomers) was 38. Linearity was between 0.5 and 10 mg/L. Precision (%RSD) of the hRf value of the aloin band was 0.88. The limit of detection was 1 µg/band. Recovery (by standard addition) was 87.6 % for aloin.

J. Planar Chromatogr. 22, 385-387 (2009). Ultrathin-layer chromatography of quercetin, rutin, and quercetin-3-O-glucoside on monolithic silica gel (size 30 mm x 18 mm) with binary and tertiary mobile phases in a cylindrical glass chamber previously saturated for 1 min. The migration distance was 20 mm and development time was 2 min. The investigated mobile phases were ethyl acetate - n-hexane 1:4 and 3:7, tetrahydrofurane - hexane 2:3 and 3:2, tetrahydrofurane - methanol - hexane 3:3:4, and hexane - acetone - methyl ethyl ketone 3:3:4. The best separation was achieved with acetone - methyl ethyl ketone - hexane 3:4:3. Densitometric evaluation at 366 nm.

J. Planar Chromatogr. 22, 283-286 (2009). HPTLC of withaferin and extracts of the powdered root on silica gel, prewashed with methanol, with toluene - ethyl acetate - acetone 2:3:3 in a twin trough chamber saturated with mobile phase for 30 min. Detection by spraying with anisaldehyde reagent followed by heating for 15 min at 105 °C; characteristic orange fluorescence was observed for whithaferin. Quantitative determination by absorbance measurement at 214 nm. The limit of detection and quantification for withaferin A was 258 and 782 ng/zone, respectively.

60th Indian Pharmaceutical Congress PA-223 (2008). HPTLC of curcumin, demethoxycurcumin and bis-demethoxycurcumin on silica gel with chloroform - methanol 19:1. The hRf values were 25, 38, and 61 for bis-demethoxycurcumin, demethoxycurcumin, and curcumin respectively. Quantitative determination by absorbance measurement at 420 nm. The method was linear in the range of 50-400 ng/spot (curcumin), 10-150 ng/spot (demethoxycurcumin), and 5-40 ng/spot (bis-demethoxycurcumin). Recovery was in the range of 99.2-100.5 % for all three compounds.

J. Chinese Trad. & Herb. Drugs 40 (6), 900-903 (2009). TLC of the TCM drug extracts on silica gel with 1) ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:2; 2) petroleum ether (30-60 °C) - ethyl acetate - formic acid 15:5:1; 3) chloroform - ethyl acetate - methanol - water 31:81:25:2. Detection 1) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C until coloration; 2) under UV 254 nm.