J. Planar Chromatogr. 14, 191-193 (2001). HPTLC of convallatoxine on silica gel by multiple-gradient thin-layer chromatography with ethyl acetate - methanol - acetic acid. Development 'face down' in a horizontal chamber. Detection of spots with several reagents reported in the literature; quantitation by densitometry at 366 nm.

J. Planar Chromatogr. 15, 128-131 (2002). HPTLC of chrysophanol on silica gel with hexane - ethyl acetate 9:1. Detection and quantification by densitometry at 366 nm. Simple, specific, and efficient method.

J. Planar Chromatogr. 15, 463-465 (2002). TLC of phenolic acids (3,5-dihydroxybenzoic acid, vanillic acid, p-coumaric acid, p-hydroxybenzoic acid, gentisic acid, caffeic acid, syringic acid, sinapic acid, ferulic acid, protocatechuic acid, 2,4-dihydroxybenzoic acid) on RP-18 (first development) and in the perpendicular direction on silica gel. One-dimensional TLC on RP-18 with methanol - water 2:3, on silica gel with toluene - dioxane - formic acid 7:2:1 and with a mobile phase I, a gradient of decreasing polarity and formic acid concentration. Detection under UV 254 and 366 nm and by coupling with bis-diazotized sulfanilamide.

leaves, inflorecences and rhizomes. J. Planar Chromatogr. 16, 230-236 (2003). Optimization of separation TLC of free phenolic acids and those released after acidic and basic hydrolysis (gallic, chlorogenic, protocatechuic, m- and p-hydroxybenzoic, vanillic, isovanillic, caffeic, gentisic, syringic, o-, m-, and p-coumaric, ferulic, salicylic, a- and g-resorcylic, sinapic and veratric acid used as standards) by one- and two-dimensional development on cellulose, polyamide 11 and silica gel with different mobile phases. Detection under UV 254 and 366 nm, then in daylight after treatment with diazotized sulfanilic acid, both with and without spraying with 20% sodium carbonate solution. Densitometry. Phenolic acids can be successfully separated on polyamide 11 with benzene - methanol - acetic acid 45:8:4.

J. Planar Chromatogr. 16, 377-380 (2003). TLC of diosmin on silica gel with chloroform - methanol - water 23:12:2 with chamber saturation with 25% NH3. Quantitation by densitometry at 344 nm. Repeatable and accurate results were obtained, limit of detection was 20 ng; satisfactory recovery (99.8 to 100.3%); linearity is given from 5 to 50 mg/mL. New and simple procedure.

CBS 90, 10-11 (2003). HPTLC of periwinkle cell samples on prewashed silica gel with ethylacetate - diethylamine 9:1 in horizontal developing chamber over 25 mm. Detection by radiation with short-wave UV 254 nm. Quantitative determination by fluorescence measurement at 254 nm or 365 nm.

CBS 88, 14-15 (2002). HPTLC of mushroom extracts on silica gel with dichloromethane - ethyl acetate - methanol 3:1:1 and HPTLC of hydroquinone, rutin, resorcinol, and ascorbic acid on silica gel with toluene - methanol - acetic acid 45:8:4 over 80 mm with chamber saturation. Determination of bioactivity with DPPH-biotest by spraying with 5 mg (2,2-di-(4-tert-octylphenol)-1-picrylhydrazyl in 10 mL acetone.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (8), Append. 9-11 (2004). TLC on silica gel previously immersed in 0.5 % NaOH solution, developed with 1) ethyl acetate - methanol - water 100:17:13; and 2) with the upper phase of toluene - ethyl acetate - formic acid - water 20:10:1:1. Detection by spraying with AlCl3 solution and under UV 365 nm. Identification by fingerprint technique and comparison with the standard. Quantification of scutellarin by HPLC.