J. Chromatogr. A 1524, 266-272 (2017). Demonstration of the antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds. Investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria by TLC and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography. Transfer of antibacterial fractions obtaining infusion-transfusion OPLC to HPLC-MS/MS analysis resulted in the characterization of three active compounds, two of them were identified as linoleic and linolenic acid. Adoption of OPLC method to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide, sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

J. Chromatogr. 312, 357-385 (1984). Two-dimensional TLC of dansylated basic nitrogen fractions (BNF) on silica with cyclohexane - ethyl acetate 2:3 and with benzene - triethylamine 5:1. Detection by UV 366 nm. Dansylation of the plant amines was carried out prior to TLC with DNS chloride in acetone (5 mg/ml) in the presence of excess solid NaHCO3. Standards for comparison were separated with chloroform - butylacetate 8:3 and with cyclohexane - benzene - methanol 2:7:1. Rf values of more than 80 plant amines are reported.

Pharm. Anal. (Yaowu Fenxi Zazhi) 5, 18-20 (1985). (Chinese) (TLC-UV spectrophotometric determination of paeoniflorin in Chinese paeonia medicinal herbs and Ming Mu Xiao Yao San preparations.) TLC of paeoniflorin on silica. The spot was eluted with ethanol. Determination by spectrophotometry at 234 nm. Approx. 100 % recovery.

Detection by spraying with 10 % sulfuric acid. Quantification by densitometry at 460 nm.

Helv. Chim. Acta 69, 2038-2047 (1986). TLC of triterpene glycosides on silica with chloroform - methanol - water 58:35:7 and 70:30:3. Also HPTLC on RP-8 with methanol - water 6:4. Detection with Godins reagent. Bayogenin derivatives shoved a blue color, whereas those of oleanolic acid were purple.

J. High Resol. Chromatogr. 10, 362-364 (1987). Application of the top triangle of the „prisma“ model for mobile phase optimization of polar compounds in normal phase chromatography. TLC and HPTLC of ginsenosides on silica with water - methanol - MEK in various proportions. OPLC of the separated ginsenosides on silica with MEK - methanol - water 70:22:8. Detection by spraying with vanillin - sulfuric acid and densitometry at 550 nm.

I. The culture of Callus and the analysis of active components) (Chinese). J. Chinese Herb Med. 18, 367-370 (1987) (Zhong Caoyao). TLC of andrographolide and neo-andrographolide on silica with chloroform - ethanol 4:1 and ethyl acetate - ethanol 6:1. Detection by exposing to iodine vapor, or spraying with sulfuric acid and Kedde reagent.

J. Chinese Trad. Med. 12, 301-302 (1987) (Zhongyao Tongbao). TLC on silica with ethyl acetate - methanol - water 20:3.4:2.7. Quantification by densitometry at 290 nm and 720 nm, resp.