CBS 90, 10-11 (2003). HPTLC of periwinkle cell samples on prewashed silica gel with ethylacetate - diethylamine 9:1 in horizontal developing chamber over 25 mm. Detection by radiation with short-wave UV 254 nm. Quantitative determination by fluorescence measurement at 254 nm or 365 nm.

CBS 88, 14-15 (2002). HPTLC of mushroom extracts on silica gel with dichloromethane - ethyl acetate - methanol 3:1:1 and HPTLC of hydroquinone, rutin, resorcinol, and ascorbic acid on silica gel with toluene - methanol - acetic acid 45:8:4 over 80 mm with chamber saturation. Determination of bioactivity with DPPH-biotest by spraying with 5 mg (2,2-di-(4-tert-octylphenol)-1-picrylhydrazyl in 10 mL acetone.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (8), Append. 9-11 (2004). TLC on silica gel previously immersed in 0.5 % NaOH solution, developed with 1) ethyl acetate - methanol - water 100:17:13; and 2) with the upper phase of toluene - ethyl acetate - formic acid - water 20:10:1:1. Detection by spraying with AlCl3 solution and under UV 365 nm. Identification by fingerprint technique and comparison with the standard. Quantification of scutellarin by HPLC.

J. Planar Chromatogr. 18, 34 -38 (2005). Considering the latest technical and methodological developments, modern high-performance thin-layer chromatography, also known as planar chromatography, is a reliable and powerful analytical technique, in full compliance with current good-manufacturing practice (cGMP). With the proper equipment TLC is the method of choice when many samples must be analysed at low cost per sample. Advantages of HPTLC are shown in the analysis of botanicals: 1) Identification (separation of Stephania tetrandra root extracts with tetrandrine as standard on silica gel with toluene - ethyl acetate - methanol -ammonia 100:100:50:3; detection under UV at 254 and 366 nm, under white light after derivatization with iodine, and under UV at 366 nm after derivatization with anisaldehyde. 2) Semi-quantitative assessments in process control and stability tests (separation of fatty acids of Saw Palmetto products on RP-18 by two fold development with dichloromethane - acetic acid - acetone 2:4:5. 3) Quantitfication of marker compounds, like curcumin measured at 366 nm/>400 nm on silica gel with toluene - acetic acid 4:1. 4) Choice of stationary phase (separation of flavonoids on conventional TLC plates and on HPTLC plates with formic acid - water - ethyl methyl ketone - ethyl acetate 10:10:30:50 and detection with natural products reagent; switching to HPTLC reduced analysis time to a quarter and gave sharper bands). 5) Choice of mobile phase; 6) Derivatization and 7) Chromatogram evaluation.

Planta Med. 71, 194-196 (2005). Analytical TLC of glaucopines A and B on silica gel with dichloromethane - methanol 19:1. Detection by spraying with 50 % sulfuric acid.

Planta Med. 70, 993-1000 (2004). Preparative TLC of catuabine and its hydroxymethyl derivative 7-exo-hydroxy-N-methyl-catuabine on silica gel with dichloromethane - acetone 97:3 and toluene - acetone - methanol - ammonia 45:45:7:3. Detection under UV light at 254 and 366 nm and by spraying with potassium iodoplatinate reagent (0.25 mL of 5 % hexachloroplatinic acid solution mixed with 2.25 mL of 10 % potassium iodide solution and dissolved with 5 mL water).

Planta Med. 72, 187-189 (2006). Preparative TLC of shinpherocarpin, 7,4’-dihydroxy-2’,5’-dimethoxyisoflav-3-ene, and 7-hydroxy-4’-methoxy-3’-prenylisoflavone (5-deoxy-3’-prenylbiochanin A) on silica gel with hexane - dichloromethane 3:7 (multiple development). Detection under UV light at 254 nm.

Phytochem. Anal. 17, 164-166 (2006). HPTLC of gymnemagenin on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity of the determination of gymnemagenin was observed in the range of 4 - 10 µg. The average percentage recovery from an extract was 99.1 %, the content of leaves was 1.61 % (dry weight).