J. Liq. Chromatogr. Relat. Technol. 28, 2489-2491 (2005). 2-D TLC of 4 ecdysteroids and several flavonoids on cyano phase with toluene - acetone - ethanol - 25 % ammonia 100:140:32:9 and ethyl acetate - ethanol - water 16:2:1. Detection under UV 254 nm, and under white light and UV 366 nm after spraying with vanillin-sulfuric acid reagent followed by heating. TLC of L-deprenyl and 14C-L-deprenyl((-)-N-methyl-N-propynyl(2-phenyl-1-methyl)ethylammonium hydrochloride) on silica gel with chloroform - methanol - water 7:5:1, and dichloromethane - triethanolamine 19:1 for elution and displacement, that is for the first and second dimensional developments, respectively.

J. Liq. Chromatogr. & Relat. Technol. 30, 1105-1114 (2007). TLC of salvinorin A, a potent naturally occuring kappa-opioid selective agonist, on silica gel and on RP-18 with n-hexane - ethyl acetate 1:1. Detection by spraying with vanillin - phosphoric acid reagent followed by heating.

Br. by High-Performance Thin-Layer Chromatography. J. Planar Chromatogr. 20, 117-120 (2007). HPTLC of ursolic acid (3beta-hydroxyuro-12-enoic acid) on silica gel prewashed with methanol in a twin-trough chamber with toluene - ethyl acetate - triethylamine - methanol 7:2:1:1. After derivatization with Liebermann-Burchard reagent the chromatograms were evaluated densitometrically at 366 nm in the fluorescence mode.

59th Indian Pharmaceutical congress C-344, 308, (2007). TLC of different fractions of fruits of Azadirecta indica on silica gel with ethyl acetate - n-hexane 1:1. The MIC value was determined in comparison with gedunin isolated from the plant. The fungi were sub cultured and incubated for seven days, then sprayed on the developed plate, followed by incubation at 37°C . Zones of inhibition were measured. Antifungal activity was observed with Aspergillus niger, Fusarium specis, and Penicillium chrysogenum.

Indian Drugs 44(10), 751 (2007). TLC and HPTLC of methanolic extracts of Eclipta alba, Launaea nudicaulis and Tridax procumbens, on silica gel with toluene - ethyl acetate - methanol 14:5:1. Evaluation under 254 and 366 nm. Detection by spraying with anisaldehyde sulfuric acid followed by evaluation at 525 nm.

Phytochem. Anal. 19, 116-121 (2008). HPTLC of cinnamaldehyde (1) in the shoots of Cinnamomum zeylanicum, eugenol (2) in the flower buds of Eugenia caryophyllus and piperine (3) in the fruits of Piper nigrum on silica gel with petroleum ether - dichloromethane - formic acid 20:40:1. Quantitative determination by absorbance measurement at 290 nm. The hRf values were 47, 61, and 12 for (1), (2), and (3), respectively. Linearity was between 54 and 735 ng/spot for (1), 533 and 8531 ng/spot for (2), and 50 and 300 ng/spot for (3). The limits of detection and quantification were 12 and 21 ng/spot for (1), 240 and 426 ng/spot for (2), and 18 and 40 ng/spot for (3). Recovery was 99 % for each substance. No significant intra- and interday variation was observed. The method proved to be rapid and useful in comparison with GC and HPLC methods.

J. Planar Chromatogr. 21, 21-26 (2008). HPTLC of extracts of Hoodia gordonii with fructose and beta-sitosterol as standards on silica gel with chloroform - methanol - water 70:30:3 in an automatic developing chamber fitted with a twin-trough chamber. The chamber was saturated for 20 min with mobile phase and relative humidity was controlled (33 %). Detection by dipping in anisaldehyde reagent, followed by heating at 100 °C for 3 min. Documentation and evaluation before derivatization under UV 366 nm and after derivatization under white light. The method was validated. It is specific and allows discrimination of Hoodia gordonii from Hoodia currorii, Hoodia parviflora, and the common adulterant prickly pear cactus (Opuntia ficus-indica). The sample is stable in solution and on the plate for at least 3 h, as well as during chromatography (2D test). After derivatization the chromatogram is stable for at least 1 hour. Precision (repeatability, intermediate precision, and reproducibility was good and the method is robust . The method is sensitive to changes in relative humidity. If relative humidity exceeds 47% the plate must be conditioned to 33% RH to ensure proper separation.

Abstract No. 9147, IHCB (2009). HPTLC of quercetin in methanolic leaf extracts of Viola odorata on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Quantitative determination by absorbance measurement at 366/>400 nm for quantification. The extract contained 0.36 % quercetin.