Acta Chromatographica 21(3), 443-452 (2009). HPTLC on silica gel with ethyl acetate – hexane 3:17. Detection and quantification by densitometry at the maximum absorbance wavelength of 250 nm. The linearity was in the range of 60–260 ng/zone with r=0.9997. The limits of detection and quantification were 20 and 60 ng/zone, respectively. The precision and repeatability of the method were found to be 0.8 and 1.1 %, respectively. Recovery ranged from 97.9-100.1 %. The maximum zerumbone content in the rhizome was 1.81 %.

Planta Med. 75, 1618-1624 (2009). TLC of gentiopicroside and methanolic plant extracts on silica gel containing 1 % CMC-Na with ethyl acetate - ethanol - water 20:2:1. Detection by spraying with 2 % vanillin-sulfuric acid reagent and by evaluation under UV 254 nm.

J. Planar Chromatogr. 24, 373-375 (2011). HPTLC of red clover capsule extracts and formononetin, biochanin A, daidzein, glycitein, and genistein on silica gel, prewashed with methanol, with dichloromethane - glacial acetic acid - ethyl acetate 12:2:1 in a horizontal chamber saturated for 15 min. Quantitative determination by densitometry at 260 nm. The hRf value was 29, 34, 41, 48, and 59 for daidzein, glycitein, genistein, formononetin, and biochanin A, respectively. The two major isoflavones are formononetin and biochanin A. The limit of detection and quantification was 14 and 47 ng/band for formononetin and 12 and 40 ng per band for biochanin A, respectively. The recovery was 93.3-100.7 % for formononetin and 102.0-109.4 % for biochanin A.

PHCOGJ 2(11), 374-380 (2010). Root bark of Alangium salvifolium (Linn.f.) Wang is a reputed drug mentioned in the ancient books of Ayurveda and Siddha for the treatment of epilepsy, jaundice, hepatitis etc. TLC of ethanolic, chloroform, and ethyl acetate extracts of the root bark of Alangium salvifolium on silica gel with toluene - ethyl acetate - diethylamine 10:7:1. Evaluation under UV 254 nm and 366 nm.

J. of China Pharm. 20 (9), 16-17 (2011). TLC of the extracts of Biyan syrup 1) for Herba Ephedrae, on silica gel with chloroform - methanol - ammonia 40:10:1, detection by spraying with ninhydrin reagent and heating at 105 ºC; 2) for Flos Magnoliae, on silica gel with chloroform - diethyl ether 5:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 3) for Radix Astragali, on silica gel with ethyl acetate - butanone - formic acid - water 5:3:1:1, detection by spraying with 5 % iron(III) chloride in ethanol; 4) for Angelica dahurica (Fisch.) Benth. et Hook, on silica gel with toluene - petroleum ether (60-90 ºC) - ethyl acetate 6:3:1, detection under UV 366 nm.

J. Strait Pharm. 22 (12), 59-60 (2010). TLC of the extracts of the title traditional Chinese medicine 1) for red Ginseng, on silica gel with benzene - ethyl acetate - formic acid 20:16:3, detection by spraying with 3 % FeCl3 in ethanol and viewing under daylight; 2) for Chinese Taxillus twig, on silica gel with water-saturated toluene - ethyl acetate - formic acid 5:4:1, detection by spraying with 5 % AlCl3 in ethanol; 3) for Hawthorn, on silica gel with toluene - ethyl acetate - glacial acetic acid 24:8:1, detection by spraying with 3-10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected; 4) for Alisma orientale, on silica gel with toluene - ethyl acetate - formic acid 14:7:2, detection by spraying with acetic acid - concentrated sulfuric acid - ethanol 1:1:1, heating at 105 °C until the zones were detected and viewing under daylight. Identification by comparison with the standards of the individual drug.

Chinese J. of Ethnomed. & Ethnopharm. 23, 61-62 (2010). Preparation of the samples by extracting Saururus chinensis (Lour.) Baill with methanol - 25 % hydrochloric acid 4:1 and sonication for 1 h (these were the best conditions of five different solvent compositions and different sonication times investigated). TLC of the obtained extracts on silica gel with 1) petroleum ether (60-90 ºC) - acetone 5:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 2) toluene - ethyl acetate - formic acid 5:2:1, detection by spraying with 1 % aluminium chloride in ethanol and evaluation under UV 366 nm; 3) hexane - ethyl acetate - formic acid 70:50:8, detection by spraying with 1 % aluminium chloride in ethanol and heating at 105 ºC, detection under daylight and UV 366 nm; 4) toluene - ethyl acetate - formic acid 5:4:1 saturated with hydrochloric acid, detection under daylight and UV 366 nm. System 4) provided the best separation and was most practical. Identification of quercetin by fingerprint comparison with the standard.

J. Planar Chromatogr. 25, 420-425 (2012). HPTLC of juglone (1), quercetin (2), myricetin (3), rutin (4), caffeic acid (5), and gallic acid (6) in the stem bark of Juglans regia L. on RP-18 with methanol - water - formic acid - acetic acid 61:58:3:3. Quantitative determination by absorbance measurement at 254 nm. The hRf values of compounds (1) to (6) were 16, 20, 31, 42, 55 and 78, respectively. Linearity was in the range of 13-800 ng/zone for (1) and (5), 100-1000 ng/zone for (2) and (6) and 50-800 ng/zone for (3) and (4). Limits of detection and quantification were 4 and 13 ng/zone for (1) and (5), 30 and 100 ng/zone for (2), 15 and 50 ng/zone for (3), (4) and (5), respectively. The intra-day and inter-day precisions for compounds (1) to (6) were in the range of 0.6-1.3 % and 0.5-1.9 %, respectively. Average recovery was between 98.6 and 101.1 %.