China Pharm. 22 (20), 21-23 (2013). Clematis L. is a traditional mongolian medicines. HPTLC identification of C. aethusifolia, C. intricate, C. brevicaudata, C. hexapeta, C. macropetala and C. mandshurica on silica gel with (A) petroleum ether (60-90°C) – ethyl acetate – acetone 9:3:1 at 25 °C and relative humidity of 50 %, detection by spraying with sulfuric acid – water 1:3, followed by heating at 100 °C until the spots were visible, or (B) chloroform – methanol – water 30:6:1 at 25 °C and relative humidity of 50 %, detection by spraying with sulfuric acid – water 1:3, followed by heating to 100 °C and viewing at UV 366 nm.

Pharm. Clinics Chinese Materia Med. 4 (6), 21-23 (2013). Lishen Jiaonang capsule is a TCM preparation for treatment of renal diseases. For quality control, TLC on silica gel 1) for Equisetum hyemale L. and the standard kaempferide with cyclohexane – ethyl acetate – formic acid 20:10:1, detection by spraying with 5 % aluminum trichloride in ethanol and evaluation at UV 366 nm; 2) for Heartleaf Houttuynia Herb with benzene – chloroform – acetone 8:5:7, detection at UV 366 nm. Quantification of kaempferide by HPLC.

Food Chem. 187, 460-468 (2015). HPTLC-direct bioautography of bioactive compounds in the extracts of cold-pressed hemp (1), flax (2) and canola (3) seed oil on silica gel with toluene - ethyl acetate - formic acid - water 15:30:5:3 for (2) and toluene - ethyl acetate - acetic acid 80:25:4 for (1) and (3). HPTLC-DPPH scavenging activity was determined by dipping into a methanolic DPPH solution, followed by drying for 90 s in the dark and heating at 60 °C for 30 s. The hRF values of dominant radical scavenging zones were in the range of 75-85 for (1), 70-90 for (2) and 64 and 95-100 or (3). HPTLC-antimicrobial Aliivibrio fischeri assay allowed the determination of major antimicrobial zones at hRF 40-49 and 55-66 or (1), 23, 45 and 60 for (3) and 95 for (2). Additional effect-directed analyses employing acetylcholinesterase (AChE) assay, planar yeast estrogen (pYES) bioassay and Bacillus subtilis bioassay as well as subsequent HPTLC-ESI-MS allowed targeted characterization of bioactive compounds.

J. Liq. Chromatogr. Relat. Technol. 38, 1088-1093 (2015). HPTLC of tramadol hydrochloride in pharmaceutical formulation on silica gel with ethyl acetate - methanol - ammonia 90:8:5. Quantitative determination by absorbance measurement at 271 nm. The hRF value of tramadol hydrochloride was 64. Linearity was in the range of 1000-6000 ng/zone. Intra-day and inter-day precisions were below 2 % (n=3). Mean recoveries were found to be around 100 %.

J. Liq. Chromatogr. Relat. Technol. 38, 1577-1584 (2015). Reversed-phase TLC of 54 new antiproliferative 6,9-disubstituted quinobenzothiazines on RP-18 with acetone-TRIS buffer pH 7.4 mixtures containing amounts of acetone in the range of 50-85 % (v/v) in 5 % increments. Detection at UV 254 nm. The lipophilicity parameters of these compounds were determined experimentally and compared with theoretical values.

Marine Drugs 12 (5), 2633-2667 (2014). The saponin-enriched isobutanol-soluble fraction of a prepurified ethanol – water 7:3 extract of the viscera (all intern organs after removal of the body wall) of Holothuria lessoni Massin & al. (2009) was submitted to high performance centrifugal partition chromatography (HPCPC), before direct MS injection. For the monitoring of the HPCPC, TLC of subfractions on silica gel with the lower phase of chloroform – methanol – water 7:13:8. Detection under UV light and by spraying with 15 % sulfuric acid in ethanol followed by heating at 110 °C for 15 min, and evaluation in daylight, saponins are hued in maroon/dark purple. At least 39 new saponins and 36 reported triterpene glycosides (including holothurins, argusides and leucospilotasides), containing different aglycones and sugar moieties, were identified in the viscera of this sea cucumber.

J. AOAC Int. 98, 1226-1233 (2015). HPTLC of three phosphodiesterase type 5 inhibitors, sildenafil (1), vardenafil (2), and tadalafil (3), and eight of their analogs, hydroxyacetildenafil (4), homosildenafil (5), thiohomosildenafil (6), acetildenafil (7), acetaminotadalafil (8), propoxyphenyl (9), hydroxyhomosildenafil (10), hydroxyhomosildenafil (11) and hydroxythiohomosildenafil (12) in finished products on silica gel with TBME - methanol - ammonia 28% 20:2:1. Detection under 254 and 366 nm UV light and by absorbance measurement at 232 nm. LOD was 30 ng/zone for all substances. Confirmation by HPTLC-ESI-MS. The method was successfully_x000D_ applied to screening of 45 commercial lifestyle products. Of those, 31 products tested positive for at least one illegal component (sildenafil, tadalafil, propoxyphenyl, hydroxyhomosildenafil, or dimethylsildenafil).

J. Planar Chromatogr. 28, 343-351 (2015). TLC of cobalt(II) (1) and copper(II) (2) on silica gel impregnated with cetyltrimethylammonium bromide micelles in water. The mobile phase was acetone - 3 M hydrochloric acid 99:1 with 2.6 mM hexamethylenediamine. Detection by spraying with 1 % potassium ferrocyanide for (1) or 1% dimethylglyoxime solution in ethanol for (2). Quantitative determination by absorbance measurement at 470 and 615 nm, respectively. The hRF values for (1) and (2) were 65 and 26, respectively. The LOD was 0.02 ng/L for (1) and 0.1 µg/L for (2).