J. Liq. Chromatogr. Relat. Technol. 35, 2753-2764 (2012). HPTLC of melitracen hydrochloride (1) and flupentixol hydrochloride (2) on silica gel with methanol - chloroform - toluene 2:9:9 + 1 drop ammonia. Quantitative determination by absorbance measurement at 291 nm. The hRf value of compounds (1) and (2) were 53 and 34 and selectivity regarding matrix was given. Linearity was in the range of 1600-6400 ng/band and 80-320 ng/band for (1) and (2), respectively. Limit of detection was found to be 27 ng/band and 5 ng/band for (1) and (2), respectively. Limit of quantification was found to be 82 ng/band and 14 ng/band for (1) and (2), respectively. The intermediate/inter-day/intra-day precision was below 0.2 % for (1) and 1.1 % for (2) (n=3). Recovery (by standard addition) was between 99.1 and 101.8 % for both (1) and (2). The method showed comparable results with HPLC.

J. Planar Chromatogr. 25, 559-565 (2012). HPTLC of zafirlukast on silica gel with toluene - methanol - acetone 7:2:1. Quantitative determination by absorbance measurement at 240 nm. Linearity was in the range of 50-400 ng/zone. Limits of detection and quantification were 16 and 50 ng/zone, respectively.

J. Planar Chromatogr. 25, 523-527 (2012). TLC of a mixture of 12 non-steroidal anti-inflammatory drugs on silica gel with ethyl acetate - toluene - methanol - acetic acid 40:40:1:1. Quantitative determination by absorbance measurement at 254 nm. Limits of detection were in the range of 5-500 ng/zone. The method showed comparable results to a validated HPLC method.

J. of Qilu Med. & Pharm. 31 (6), 327-330 (2012) Zhachong Shisanwei Pill is a herbal TCM preparation for invigorating the circulation of blood. TLC on silica gel 1) for Fructus Chebulae and the standard gallic acid, with chloroform – ethyl acetate – formic acid 3:2:1, detection by spraying with 2 % ferric chloride in ethanol and heating at 105 ºC until the zones are visible in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with chloroform – cyclohexane 5:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 ºC until the zones are visible in daylight; 3) for Aquilaria agallocha (Lour.) Roxb, with toluene – ethyl acetate 4:1, detection under UV 366 nm; 4) for Rhizoma Acori Tatarinowii, with petroleum ether (60-90 ºC) – ethyl acetate 8:3, detection under UV 366 nm.

J. Planar Chromatogr. 26, 147-152 (2013). TLC of poly(methylmethacrylate) (PMMA) on silica gel with acetonitrile binary solvent mixtures with carbon tetrachloride, toluene, methylene chloride, chloroform and dichloroethane. Mark-Kuhn-Houwink parameters for PMMAs, Snyder’s parameters and the measured intrinsic viscosity values for PMMA. The characteristics of adsorption-exclusion transition depends both on thermodynamic affinity of the solvent for the polymer and macromolecule-to-pore size ratio.

J. Planar Chromatogr. 26, 165-171 (2013). OPLC of essential and non essential amino acids in proteins like hen egg yolk protein, bovine serum albumin (BSA), and Type I Collagen from bovine achilles tendon on silica gel with n-butanol - glacial acetic acid - water 40:3:12 and 50 bar external pressure, 300 µL rapid eluent flush, 100 µL/min eluent flow rate, 3200 µL development volume and 1950 s developing time. Detection by spraying first with 4-hydroxybenzaldehyde (1 % in acetone) and secondly, with ninhydrin (0.25 % in acetone), followed by heating at 110 °C for 10 min. Qualitative determination by absorbance measurement at 460 nm.

Modern J. of Integrated Trad. Chinese & Western Med. 22 (6), 659-661 (2013). Niuhuang Jiedu Ruanjiaonang capsules are a herbal TCM preparation for treating sore throat, gingival swelling and pain, aphthae, swollen red eyes, etc. For quality control, TLC on silica gel 1) for Artificial Calculus Bovis and the standards cholic acid and hyodesoxycholic acid, with n-hexane – ethyl acetate – acetic acid – methanol 20:25:2:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 2) for Radix et Rhizoma Rhei, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm; 3) for Radix Scutellariae and the standard baicalin, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 1 % ferric chloride in ethanol and viewing in daylight; 4) for Borneol, with cyclohexane – ethyl acetate 4:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight. Quantification of borneol by GC.

by thin-layer chromatography) (Chinese). J. of Trad. Chinese Med. & Pharm. Consult. 4 (5), 481-482 (2012). As a widely used TCM herbal drug, Polygonum orientale Linn. is prescribed for treatment of rheumatic arthritis, coronary heart disease, stomach pain, etc. Description of a method for identifying the counterfeit drug and for routine quality control of the crude drug by identification of the flavonoids quercetin and kaempferol in the drug samples obtained by extraction of the dry stem, leaves and fruits of the herb after acid hydrolysis. TLC on silica gel with toluene – ethyl acetate – formic acid 20:14:1, detection by spraying with 1 % aluminium chloride in ethanol and heating mildly, viewing under UV 366 nm.