J. Chromatogr. Sci. 53 (2), 312-319 (2015). Investigation of lipophilicity for newly synthesized N-substituted-2-phenylacetamide derivatives by TLC on RP-18 in the presence of one protic (methanol) and one aprotic solvent (acetonitrile) followed by mathematical evaluation using different software packages. Establishment of the dependences between the partition coefficient, log P as the standard measure of lipophilicity and the alternative lipophilic parameters using the retention chromatographic constant RM0 and m values (the intercept and the slope of the linear dependence between volume fractions of the organic solvent and the retention parameters obtained experimentally by TLC), employing linear regression analysis and multivariate methods, cluster analysis (CA) and principal component analysis (PCA). All applied methods gave approximately similar results. Although there is a linear dependence between the two chromatographic parameters RM0 and m, only RM0 shows suitable similarity with the standard measure of lipophilicity of the investigated N-substituted-2-phenylacetamide derivatives at the given conditions. This proves that the chromatographic retention constant, RM0, obtained by reversed phase TLC could be successfully used for the description of lipophilicity of the compounds. The results confirmed that the applied linear regression analysis and the multivariate analysis (CA and PCA) have the ability to compare lipophilic parameters of the investigated phenylacetamide derivatives obtained in different ways.

J. Sep. Sci. 38, 4180-4186 (2015). HPTLC of glycyrrhizic acid in the dried root and rhizoma of the plants Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, and Glycyrrhiza glabra L. on silica gel with ethyl acetate - water - acetic acid - formic acid 14:2:2:1. Detection by spraying with 10 % solution of sulfuric acid in ethanol, followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 365 nm.

J. Planar Chromatogr. 28, 395-397 (2015). HPTLC of 25-B-NBOMe (a N-(2-methoxy)benzyl-substituted phenylethylamine hallucinogen) in seized blotters (small, square pieces of absorbant paper impregnated with LSD or other hallucinogens) on silica gel with cyclohexane – toluene – diethylamine 15:3:2. Quantitative determination by absorbance measurement at 298 nm. The hRF value for 25-B-NBOMe was 34. Linearity was in the range of 19-115 μg/zone. LOD and LOQ were 7 and 22 μg/zone. The intermediate precision was below 6.3 % (n=6). Average recovery was 98 %.

J. Ethnopharmacol. 190, 116-141 (2016). This review discusses the progress in chemical analysis of Angelica sinensis and its preparations. The study describes TLC as a rapid separation method and qualitative analysis technology that is commonly used for routine chemical analysis of A. sinensis. The advantages of the technique as well as TLC systems using ferulic acid and ligustilide as markers were described.

Food Chem. 214, 162-168 (2017). HPTLC of cinnamic acid (1), ferulic acid (2), caffeic acid (3) and flavonoid-glycosides (4) in the grains of Kodo millet (Papsalum scrobiculatum) on silica gel with ethyl acetate – formic acid – water 30:2:2. Detection by exposure to iodine vapor. The hRF values for (1) to (4) were 52, 15, 7 and 10, respectively.

Planta Medica 82, 11/12, 1117-1121 (2016). TLC was applied for two purposes: first, to monitor the subfractionation of the methanol extract of the whole Calamus acanthophyllus (eluent and layer not given); second, to analyze the sugar composition of callaphylloside (a weakly cytotoxic pregnastanol saponoside). For this purpose, callaphylloside was heated for 3 h with HCl 2 N under reflux; TLC of the lyophilized and neutralized ethyl acetate extract of the mixture on silica gel with ethyl acetate – n-butanol – water 2:7:1 together with standards. The sugar moiety consisted in rhamnose and glucose (2 units of each, as confirmed through NMR of the compound)._x000D_

Chinese J. of Ethnomed. & Ethnopharm. (10), 57-59 (2013). Sendeng-9 Tang decoction is a traditional Mongolian herbal preparation for treatment of joint swelling and pain. For quality control, TLC of its extracts (1) for Cortex Phellodendri Chinensis, on silica gel with benzene – ethyl acetate – methanol – isopropanol – ammonia 12:6:3:3:1 after saturation with ammonia vapor, detection under UV 365 nm; (2) for Sophora flavescens, on 2 % NaOH-containing silica gel with the lower phase of chloroform – methanol – ammonia 50:6:3 (stored for a night at < 10 ºC) and detection by spraying with (A) 5 % potassium bismuthate solution, (B) 5 % sodium nitrite in 70 % ethanol, and viewing in daylight; (3) for Gardenia jasminoides Ellis, on silica gel with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 ºC until the zones are visible in daylight.

J. Liq. Chromatogr. Relat. Technol. 39, 674-681 (2016). HPTLC of a group of thiouracil azo dyes on RP-18 with different mixtures of water and ethanol, isopropanol, dioxane, or tetrahydrofurane. Detection under UV light at 254 nm. Compounds lipophilicity was examined as key molecular descriptor of their potential biological activity.