J. of Chromatogr. A 1218 (33), 5693-5704 (2011) A micro-TLC platform for the fast analysis of low-molecular mass compounds from spirulina samples was developed. The target compounds were extracted with methanol, acetone or tetrahydrofuran. HPTLC on RP-18W with acetone - n-hexane 3:7 in an unsaturated chamber using a temperature controlled micro-planar chromatographic device based on a horizontal chamber. Detection under visible light before and after exposure to iodine vapor. Pictures of the chromatograms were acquired with an office scanner and digitalized. The quantitative data was analyzed using cluster analysis and principal components analysis. With this method it was possible to distinguish genuine spirulina and non-spirulina samples as well as fresh and expired commercial products.

Research J. Pharm. and Tech. 3(4), 1106-1108 (2010). TLC of spironolactone and torsemide in combined tablet dosage form on silica gel with n-hexane - ethyl acetate - methanol - glacial acetic acid 12:6:3:1. Quantitative evaluation by absorbance measurement at 263 nm. The hRf value of spironolactone and torsemide was 67 and 34, respectively. The linearity was in the range of 100-1000 ng/band for both drugs. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation.

Indian drugs 48 (02), 43-47 (2011). TLC of curcumin and 3-acetyl-11-keto-beta-boswellic acid on silica gel with chloroform - methanol 37:3 for curcumin and n-hexane - ethyl acetate 1:1 for boswellic acid derivatives. The hRf value of 3-acetyl-11-keto-beta-boswellic acid was 24 and of curcumin 59. Densitometric evaluation at 430 nm for curcumin and 254 nm for the acid. The method was linear in the range of 100-500 ng/band for curcumin and 1500-4000 ng/band for 3-acetyl-11-keto-beta-boswellic acid.

f. ) Etting, (Loranthaceae), growing on different substrates. J. Planar Chromatogr. 24, 60-65 (2011). HPTLC of phenolic compounds with caffeic acid, (+)-epicatechin, ellagic acid, gallic acid, and kaempferol as markers on silica gel with toluene - ethyl acetate - methanol - formic acid 14:6:1:1 in a twin-trough chamber with saturation for 30 min at 24 °C. Quantitative determination by absorbance measurement at 300 nm. Detection by dipping in anisaldehyde-sulfuric acid reagent followed by heating at 110 °C for 5 min. Evaluation under UV 254 nm and visible light after derivatization. Repeatability (n = 7) was between 0.5-2.4 %; intermediate precision was between 1.5-4.4 %. For (+)-epicatechin, ellagic acid, gallic acid, caffeic acid, and kaempferol, LOD was 332, 225, 21, 64, and 35 ng/zone, LOQ was 1157, 740, 67, 242, and 115 ng/zone, and precision (%RSD) was 3.8, 4.9, 4.7, 5.4, 1.7 %, respectively. The hRf value was 39 for (+)-epicatechin, 43 for ellagic acid, 55 for gallic acid, 65 for caffeic acid, and 72 for kaempferol.

J. Planar Chromatogr. 24, 348-351 (2011). Study of the lipophilicity and specific hydrophobic surface area of fourteen 1,3-benzoxazol-2(3H)-ones substituted in the benzene ring (fluoro-, chloro-, bromo-, dibromo-, amino-, and nitro-derivatives). TLC on RP-18 with methanol - water, methanol - aminoacetic acid buffer pH 2.7, and methanol - aminoacetic acid buffer pH 11.6. The concentration of methanol in the mobile phase ranged from 30-90 % in all cases. Detection under UV 254 nm. The linear correlation between the volume fraction of methanol and values over a limited range were established with good correlation coefficients (r > 0.98). The obtained results were compared with calculated partition coefficients.

J. Planar Chromatogr. 25, 504-508 (2012). Physical properties of polar and non-polar solvents under electric current pulse were studied. In addition, solvents were classified according to their behavior in a pulsating electric field with applications in planar dielectrochromatography.

J. Trad. Chinese Med. & Pharm. Consult. 3 (7), 368-369 (2011). Hormones have been widely applied in clinical practice, however, overdose hormones are liable to cause general anaphylaxis, and even serious harm to physical health. For quick identification of hormones in traditional Chinese preparations, TLC of the extracts of the preparations on silica gel with chloroform – petroleum ether (30-60 ºC) – methanol 5:3:1, detection at UV 254 nm. Identification of methyltestosterone, prednisolone acetate, estrone, ethinyloestradiol, prednisolone acetate, testosterone undecanoate, fluocinolone acetonide, dexamethasone, betamethasone sodium phosphate, and hydrocortisone acetate by fingerprint comparison with the standards. The method has been applied to the real life samples of four varieties of preparation. The results were compatible with those obtained by HPLC and MS-MS.

J. of China Pharm. 21 (10), 31-32 (2012). Chuanyushaoshang cream is a herbal TCM preparation for external application for protecting wounds, promoting wound healing, antibiosis, invigorating the circulation of blood, and relieving pain. For quality control, TLC of emodin, one of the key active constituents in the extracts of the preparations, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 7:3, detection at UV 366 nm.