J. Planar Chromatogr. 18, 437-442 (2005). HPTLC of quercetin and kaempferol in horizontal chambers on silica gel (prewashed with methanol) with four mobile phases, e.g.1,4-dioxane - toluene - 85 % acetic acid 6:24:1 or on cellulose with five mobile phases. Evaluation under UV light at 254 and 366 nm before and after spraying with a 2 % solution of zirconium (IV) dichloride oxide in methanol. Quantitative determination by absorbance measurement at 373 for quercetin and at 347 nm for kaempferol.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 538-541 (2005). TLC of Biaoshi Ganmao granule extracts on silica gel developed with 1) chloroform - methanol - water 28:10:1, 2) chloroform - methanol - ammonia 40:10:1, 3) n-hexane - diethyl ether - glacial acetic acid 50:50:1, 4) ethyl acetate - methanol - water 100:17:13, 5) toluene - ethyl acetate - formic acid 20:10:1. Detection 1) under UV 365 nm, 2) by spraying with 2 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized, 3) under UV 254 nm, 4) by spraying with AlCl3 solution. Identification by fingerprint technique. Quantification of puerarin by HPLC. The results for four batches of real life sample are given.

Abstract G-29, IPC (2005). HPTLC of valdecoxib in tablets and human plasma on silica gel with methanol - water - chloroform 6:3:1. Celecoxib was used as internal standard. Quantitative determination by absorbance measurement at 254 nm. The compound was extracted from plasma with ethyl acetate showing an extraction yield of 85 %. Linearity was obtained in the range of 25-200 ng/mL, recovery rate was 99.8 % from tablets and 97.2 % from plasma.

whole-plant powder by High-Performance Thin-layer Chromatography. J. Planar Chromatogr. 19, 68-72 (2006). HPTLC of oleanolic acid on silica gel with dichloromethane - toluene - acetone - methanol 30:40:15:3. Detection by spraying with Liebermann-Burchard reagent; quantification by densitometry at 529 nm. Detection and quantitation limits were 0.1 µg and 0.5 µg, respectively. Oleanolic acid response was linear over the range 1 to 9 µg. The validated HPTLC method can be used for routine quality-control analysis of Oldenlandia corymbosa L. whole-plant powder and for quantitative determination of oleanolic acid.

Indian Drugs 42 (10), 671-674 (2005). HPTLC of desloratadine on silica gel with methanol - n-butanol - water - toluene - glacial acetic acid 20:30:10:20:1. Quantification in absorbance mode at 254 nm. The HPTLC system was quantitatively evaluated in terms of stability, precision, repeatability, specificity, accuracy and calibration, and was suitable for the analysis of desloratadine tablet dosage form. The linearity was in the range of 30-150 µg/mL with recovery of 98.8-102.0 %

Indian J. Pharm. Sci. 67 (3), 351-355 (2005). HPTLC of carbamazepine, phenytoin, and phenobarbitone in tablets on silica gel with toluene - acetone 5:2. Quantification by absorbance measurement at 217 nm. This method was quantitatively evaluated in terms of linearity, accuracy, precision, repeatability and specificity proving its utility in estimation of drug content in tablet dosage form.

Indian Drugs 42 (9), 600-603 (2005). HPTLC of cinnarizine and domperidone in tablets, on silica gel with toluene - ethyl acetate - methanol 14:1:5. Quantitative determination by absorbance measurement at 271 nm. Rf values of cinnarizine was 0.85 and of domperidone 0.4. Linearity was observed in the range of 0.1-0.4 for cinnarizine and 0.075-0.3 µg/µL for domperidone. The recoveries were in the range of 98.95-100.25 %. The tablet matrix did not interfere with the assay.

Acta Chrom. 16, 164-172 (2006). TLC of ampicillin, amoxicillin, penicillamine, benzylpenicillin and penicillin in commercial drugs (megaphen and hipenox) on self–made stannic arsenate – cellulose 1:4 layers containing 10 % CaSO4 as binder, with acetonitrile – acetic acid – chloroform 2:3:9, and acetone – acetic acid – chloroform 5:5:6. Detection by treatment with iodine vapour. The zones were extracted with methanol and spectrophotometrically determined at 460 nm using 2,3-dichloro-5,6-dicyano-p-benzoquinone as derivatization reagent.