Modern J. of Integrated Trad. Chinese & Western Med. 21 (36), 4087-4088 (2012). Bushen Huayu granules are a herbal TCM for treating osteoporosis. For quality control, TLC on silica gel 1) for E. brevicornum Maxim and the standard icraiin, with ethyl acetate – acetone – methanol – water 20:2:3:2, detection by spraying with 1 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Radix Rehmanniae and the standard 5-hydroxymethylfurfural, with petroleum ether (60-90 °C) – ethyl acetate 1:1, detection under UV 254 nm.

Chinese J. of Med. Guide 9 (11), 136-137 (2012). Honeysuckle, as a traditional Chinese medicinal herbal crude drug, is the dried flower bud of Lonicera japonica Thunb. It is effective in clearing heat and removing toxicity. For quality control, a method for the identification of chlorogenic acid and luteoloside in honeysuckle has been developed and optimized. TLC of the extracts of the crude drug on silica gel with ethyl acetate – acetone – formic acid – water 35:15:5:6, detection by spraying with 5 % aluminium chloride in ethanol, followed by 1) evaluation under UV 366 nm for luteolosid; 2) by spraying again with the upper phase of 1 % ferric chloride – 1 % potassium ferricyanide 1:1 and viewing under daylight for chlorogenic acid.

J. Anhui Med. & Pharm. 17 (2), 202-203 (2013). Banlangen granule, processed of Radix Isatidis, is a traditional Chinese herbal medicine prescribed clinically to treat sore throat, cheek swelling, acute and chronic tonsillitis and mumps. For quality control a method for the determination of leucine, a main antiviral effective component, is presented. TLC on silica gel with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C and viewing in daylight. Quantification by densitometry at 509 nm. Validation of the method by investigation of the linearity range (0.10-0.90 µg/zone, r = 0.998, n=5), precision (%RSD = 2.8 %, n=6), stability (%RSD = 0.8 %, n=6, towards single lane within one hour), and reproducibility (%RSD = 2.0 %, n=6, within plate). The recovery (by standard addition) was 98.8 % (%RSD = 1.1 %, n=6).

J. Planar Chromatogr. 26, 440-444 (2013). HPTLC of plumbagin in herbal formulation on silica gel with toluene - ethyl acetate - methanol 8:1:1. Quantitative determination by absorbance measurement at 265 nm. The hRf value of plumbagin was 70. Linearity was between 200 and 600 ng/zone. LOD and LOQ were 3 and 8 ng/zone. Average recovery (by standard addition) was found to be 100.0 %. Intermediate intra- and inter-day precision (n=6) was below 0.05 %.

J. Chil. Chem. Soc. 58, 1737-1740 (2013). HPTLC of enalapril maleate in tablets on silica gel with 1-buthanol - glacial acetic acid - water 12:3:5. Quantitative determination by absorbance measurement at 207 nm. The hRf values of enalapril and its degradation products enalapril diketopiperazine and enalaprilat were 62, 82 and 52, respectively. Linearity was in the range of 200-1200 ng/zone for enalapril. LOD and LOQ were 23 and 72 ng/zone. Intermediate intra- and inter-day precision was below 2 % (n=6).

J. of Inform. on Trad. Chinese Med. 30 (3), 61-63 (2013). Naodesheng Koufuye oral liquid is a herbal TCM preparation for treatment of cerebral arteriosclerosis and ischemic stroke. For quality control, TLC on silica gel 1) for Flos Carthami, with cyclohexane – ethyl acetate 11:6, detection by exposure to ammonia vapors and viewing under UV 366 nm; 2) for Radix Puerariae and the standard puerarin, with chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 366 nm; 3) for Rhizoma Ligustici Chuanxiong and the standard ferulic acid, with benzene – chloroform – methanol 2:2:1, detection under UV 254 nm. Quantification of ginsenoside Rb, ginsenoside Rg and notoginsenoside R by HPLC.

J. of Modern Trad. Chinese Med. 15 (4), 320-323 (2013). Leifengshi Jiaonang capsules are a herbal TCM preparation for the treatment of rheumatism, ankylosing spondylitis, and lupus erythematosus. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, heating at 105 °C and viewing in daylight and under UV 366 nm; 2) for Angelica sinensis, with petroleum ether (60-90 °C ) – diethyl ether – acetic acid 20:20:1, detection under UV 366 nm 3) for Epimedium sagittatum, with ethyl acetate – formic acid – butanone – water 10:1:1:1, detection under UV 366 nm. Quantification of ferulic acid by HPLC.

Chinese J. of Ethnomed. & Ethnopharm. 23, 51-52 (2012). Shengjing Yusi capsule is a herbal TCM preparation for treating male infertility. For quality control, TLC on silica gel 1) for Epimedium davidii Franch, with chloroform – methanol – water 13:7:2, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis, with petroleum ether (30-60°C) – ethyl acetate – formic acid 15:5:1, detection by spraying with 5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible in daylight.