International Journal of ChemTech Research 2(1), 646-652 (2010). TLC on silica gel with toluene - methanol - triethylamine 9:3:1 with chamber saturation for 30 min. The hRf value was 71. Densitometric evaluation at 282 nm. The method was linear in the range of 100-2000 ng/band with r2 = 0.9973. The repeatability of sample application and measurement of peak area (%RSD, n=6) was 0.461 % and 0.363 %. The %RSD for intra-day and inter-day variation was 0.752-0.961 % and 0.848-1.082 % respectively. The LOD and LOQ was 10 and 50 ng/zone, respectively. The average recovery was 99.5 %. The samples were subjected to stress conditions (acid, base, oxidative, thermal) and all degradation products were well resolved from the main compound. The method was found suitable for stability studies and for routine analysis of the drug in biological fluids.

J. Serb. Chem. Soc. 75(4), 513-521 (2010). A TLC method has been reported for prediction of retention behavior of several beta-diketonato complexes of cobalt(III), chromium(III), and ruthenium(III). Aim of this study was to develop a model for accurate quantitative relationships between molecular structures and retention behavior of mixed beta-diketonato complexes. TLC of 36 complexes on silica gel with four mono- and five two-component mobile phases. Quantitative structure - retention relationships (QSSRS) shall allow the prediction of retention of new solutes and structurally similar compounds, as well as their molecular description.

International Journal of ChemTech Research 2(2), 807-812 (2010). Chloroform and ethyl acetate extracts of leaves and bark of Ficus mollis (Moraceae) were subjected to TLC fingerprint profiling on silica gel with toluene - ethyl acetate - formic acid 16:2:1. Evaluation under UV 254 nm. Derivatization with vanillin-sulfuric acid reagent, followed by heating at 105 °C until colorization. In the bark 7 well-defined zones were observed, whereas in leaves 10 zones were observed. Heavy metal and mineral analysis was performed by atomic absorption spectroscopy.

J. Chromatogr. A 1217(8), 1395-1398 (2010). TLC of atenolol, propranolol and salbutamol and their enantiomers by using different modes of loading/impregnating the Cu(II) complexes of L-proline, L-phenylalanine, L-histidine, N,N-dimethyl-L-phenylalanine, and L-tryptophan on silica gel with 1) the Cu(II)-L-amino acid complex as chiral mobile phase additive, 2) ascending development of plain commercial plates in the solutions of Cu complex, and 3) using a solution of Cu(II)acetate as mobile phase additive for the commercial TLC plates impregnated by development with the amino acid solutions. Detection by exposure to iodine vapor. The detection limit was 0.18 µg for each enantiomer. For the best method performance the Cu(II)cation has to be involved.

F-247 (2010). TLC of aceclofenac and thiocolchicoside on silica gel with methanol – chloroform – water 48:1:1. The hRf values were 70 and 83 for thiocolchicoside and aceclofenac, respectively. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 30-180 ng/band for thiocolchicoside and 750-4500 ng/band for aceclofenac. The recovery was in the range of 99.2-100.0 % for both drugs.

J. Pharm. Sci. & Res. 2(2), 143-148 (2010). TLC of salbutamol sulphate (SS), bromhexine hydrochloride (BH), and etofylline (ET) in fixed dose formulation on hand-made silica gel plates with methanol – n-hexane 2:1. All three compounds were well separated with hRf values of 25 for SS, 71 for ET and 91 for BH. Detection at 254 nm as well as by exposure to iodine vapors. For spectophotometric quantification the bands of the selected drug in sample and standard mixture were scraped off, suspended in methanol, and the absorbance was measured at the maximum absorbance wavelength of each compound.

62nd Indian Pharmaceutical Congress Abstract No. F-249 (2010). TLC of risperidone and trihexyphenidyl hydrochloride on silica gel with methanol – chloroform – glacial acetic acid 160:40:0.1. Quantitative determination by absorbance measurement at 254 nm. The linearity was in the range of 20-60 µg/band and 10-30 µg/band for risperidone and trihexyphenidyl hydrochloride, respectively. The recovery was 99.4-99.9 % for both drugs.

Acta Chromatographica 21(2), 299-317 (2009). HPTLC of amtolmetin guacil on silica gel with toluene – ethyl acetate 2:3. Identification and quantification by densitometric analysis in absorbance mode at 320 nm. The samples were subjected to acidic and alkaline hydrolysis, oxidation, dry heat treatment, and photo-degradation. The method was suitable for stability studies and to study the kinetics of degradation of amtolmetin guacil.