IPC 56th 2004, Abstract No. GP-26. HPTLC of triphala, an ayurvedic formulation containing about 3.60 % of total phenolics. Separation of alcoholic triphala extracts on silica gel with n-hexane - ethyl acetate 2:1. Rf value of the main spot gallic acid was 0.04 in triphala and its formulation. The method was found to be very specific for gallic acid having a linearity range of 0.2 - 1.6 mg/mL. Several formulations analyzed by HPTLC contained 5.2 - 7.6 % of gallic acid. The reported method is suitable for estimation of gallic acid in raw material and formulations.

J. Planar Chromatogr. 18, 344-348 (2005). TLC of beta-diketonato complexes of the type M(acac) 3-n(phacphac)n and M(acac)3-n(phSacphSac)n, where M represents cobalt(III) or chromium(III), acac is the pentanedionato ion, phacphac the 1,3-diphenyl-1,3-propanedionato ion, and phSacphSac the 3-mercato-1,3-diphenylprop-2-en-1-thione ion (n= 0-3), on RP-18 with mixtures of tetrahydrofuran, acetonitrile, or acetone (as organic modifier) with water.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (4), 410-414 (2005). TLC of the extracts of the title Chinese traditional patent medicine on silica gel with 1) chloroform - ethyl acetate - methanol - formic acid 400:25:50:1 2) the lower phase of chloroform - methanol - water 32:17:5; 3) n-hexane - chloroform - water 15:8:2. Detection 1) by spraying with 5 % vanillin - H2SO4 solution and heating mildly until the spots are visualized; 2) by spraying with 2 % AlCl3 in methanol and inspection under UV 365 nm; 3) by exposing to iodine vapour. Identification by fingerprint techniques. Quantification of paeoniflorin by HPLC.

J. Planar Chromatogr. 18, 384-387 (2005). TLC of thirty-seven N-[(4-arylpiperazin-1-yl)alkyl]-2-azaspiro[4.4]nonane- and [4.5]decane-1,3-dione derivatives on RP-18 in a pre-saturated chamber with n-propanol - Tris buffer (pH 7.0) mixtures. Detection under UV at 254 nm.

Indian J. Pharm Sciences 67 (6), 687-690 (2005). HPTLC of sparfloxacin extracted with dichloromethane from plasma. A standard solution was prepared in methanol - dichloromethane 1:1. HPTLC on silica gel with chloroform - toluene - methanol - diethylamine 44:15:2:1. Quantitative determination by absorbance measurement at 301 nm. The method had a linearity range of 80-200 ng/spot with an average recovery of 89.17 %.

Indian Drugs 42 (6), 340-344 (2005). HPTLC of captopril and hydrochlorothiazide in tablets on silica gel with methanol - toluene - ethyl acetate - glacial acetic acid 2:12:6:1. Quantitative determination by absorbance measurement at 219 nm. The Rf value of hydrochlorothiazide was 0.38 and of captopril 0.57. The calibration curve response was 4-14 µg for both drugs. Recovery was determined by standard addition method. The percent recovery by area was found to be 100.25 for captopril and 99.98 for hydrochlorothiazide. The method was suitable for routine quality control of such formulations.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 859-861 (2005). TLC of Ganlu Xiaodu capsules on silica gel with chloroform - methanol - water 185:15:2. Detection under UV 254 nm. Identification by fingerprint techniques and by HPLC. Quantification of scutellarin by HPLC.

Indian Drugs 42 (7), 424-427 (2005) HPTLC of fexofenadine HCl from tablet dosage form on silica gel with dichloromethane - methanol 13:7. Quantitative determination by absorbance measurement at 260 nm. The linear detector response was observed between 0.2 and 1.0 µg. The method was validated to determine its accuracy and precision. The LOD was found to be 0.08 ng/µL, LOQ was 0.02 ng/µL. The recovery was carried out by standard addition method and was found to be 100.82 %.