Chinese J. of Henan Univ. (Med Sci.) 32 (2), 117-121 (2013). Shiming Granule is a herbal TCM preparation for invigorating blood circulation, improving liver and pulmonary functions and the eyesight. For quality control, TLC on silica gel 1) for Rhizoma et Radix Notopterygii, with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating until the zones are visible in daylight and under UV 366 nm; 2) for Bupleurum chinense, with ethyl acetate – methanol – water 8:2:1, detection by spraying with 2 % p-dimethylaminobenzaldehyde in 40 % sulfuric acid and heating at 60 °C, viewing under UV 366 nm; 3) for Rhizoma Atractylodis, with petroleum ether (60-90 °C) - ethyl acetate 20:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % sulfuric acid and heating until the zones are visible in daylight; 4) for Exocarpium Citri Rubrum, with ethyl acetate – methanol - water 100:17:10, detection by spraying with 3 % aluminiumchloride in ethanol, viewing under UV 366 nm; 5) for Radix Saposhnikoviae, with chloroform – methanol 4:1, detection under UV 254 nm; 6) for Salvia miltiorrhiza, with benzene – ethyl acetate 19:1, detection under UV 366 nm; 7) for Radix Paeoniae Rubra, with chloroform – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating until the zones are visible in daylight; 8) for Herba Equiseti Hiemalis, with cyclohexane – ethyl acetate – formic acid 20:10:1, detection by spraying with 5 % aluminium chloride in ethanol and viewing under UV 366 nm; 9) for Cuscuta chinensis Lam., with ethyl acetate – butanone – formic acid – water 11:1:1:1, detection by spraying with 5 % aluminium chloride in ethanol and heating at 105 °C until the zones are visible, and viewing under UV 254 nm; 10) for Rehmannia glutinosa Libosch, with chloroform – methanol – ammonia 40:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 11) for Rhizoma Atractylodis Macrocephalae, with cyclohexane – ethyl acetate 7:3, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:10 and heating at 105 °C until the zones arevisible in daylight; 12) for Radix Glycyrrhizae, with ethyl acetate – formic acid – water 15:3:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 13) for Radix Achyranthis Bidentatae, with chloroform – methanol 30:1, detection by spraying with 5 % phosphomolybdic acid in n-propanol and heating at 110 °C until the zones are visible in daylight.

J. Liq. Chromatogr. Relat. Technol. 37, 2872-2881 (2014). 2D-HPTLC of 17 alpha-ethinylestradiol on RP-18 with methanol – acetonitrile – water 2:2:1 in the first direction and cyclohexane – butyl acetate – methanol 8:6:1 in the second direction, both over 70 mm. With the grafted method both plates were combined in a 2D separation. Detection by dipping into a mixture of sulfuric acid (98 %) and water (1:49), followed by heating at 110 °C for 10 min. Compound was also detected using a yeast estrogen screening assay using the strain Saccharomyces cerevisiae BJ3505.

J. Liq. Chromatogr. Relat. Technol. 37, 1416-1426 (2014). HPTLC of lumefantrine (1) and artemether (2) on silica gel with toluene – ethyl acetate – acetic acid 4:15:5. Detection by dipping into a derivatization reagent (10 % each of methanolic concentrated sulfuric acid and anisaldehyde), followed by heating at 110 °C for 3 min. Quantitative determination by absorbance measurement at 519 nm. The hRF values for (1) and (2) were 55 and 70, respectively. Linearity was in the range of 60-360 ng/zone for (1) and 10-60 ng/zone for (2). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 7 and 26 ng/zone for (1) and 2 and 6 ng/zone for (2), respectively. Recoveries were between 97.6-101.3 % for both (1) and (2).

Chinese J. of Med. Guide 11 (16), 507-509 (2013). Xiaoxumingtang keli granule is a herbal TCM effective for promoting blood circulation and removing blood stasis, prescribed as an assistant drug for the treatment of stroke, hemiplegia，rheumatis, etc. For quality control, TLC 1) for Saposhnikovia divaricata (Trucz.) Schischk. and the standards isoacteoside and 4’-o-beta-glucopyranosyl-5-o-methylvisamminol on silica gel with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 254 nm; 2) for Panax ginseng C. A. Mey. and the standards ginsenoside Rg1, Re, and Rb1 on silica gel with chloroform – methanol 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight and under UV 366 nm; 3) for Ligusticum chuanxiong Hort. and the standards ligustrazine and ferulic acid on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 4) for Paeonia veitchii and the standard paeoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 5) for Ephedra sinica Stapf and the standard (1R,2S)-(-)-ephedrine hydrochloride, on silica gel with chloroform – methanol – concentrated ammonia 40:10:1, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C until the zones are visible in daylight; 6) for Scutellaria baicalensis Georgi and standard baicalin, on plates made of silica gel - sodium acetate – 0.3 % CMC 50:8:150, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation in daylight.

J. Liq. Chromatogr. Relat. Technol. 37, 2784-2799 (2014). HPTLC of atorvastatin (1), ezetimibe (2) and fenofibrate (3) on silica gel with toluene – methanol – triethylamine 16:3:1. Quantitative determination by absorbance measurement at 245 nm. The hRF values for (1) to (3) were 10, 20 and 80, respectively. Linearity was in the range of 100-350 ng/zone for (1) and (2) and 1600-5600 ng/zone for (3). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 60 and 90 ng/zone for (1), 80 and 100 ng/zone for (2) and 90 and 150 ng/zone for (3), respectively. Recoveries were in the range of 97.6-102.6 %.

J. of China Pharm. 22 (17), 32-33 (2013). Guiqi Tongru Heji compound oral liquid is a herbal TCM preparation for the treatment of postpartum hypogalactia. For quality control, TLC on silica gel 1) for Vaccaria segetalis, with chloroform – methanol – water 15:7:2, detection by spraying with fresh solution of (bismuth subnitrate - glacial acetic acid – water 17:200:800) – 40 % potassium iodate – glacial acetic acid – water 1:1:4:15, evaluation in daylight; 2) for Scrophularia ningpoensis Hemsl., with ethyl acetate - methanol – formic acid – glacial acetic acid - water 30:1:2:2:4, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones were visible in daylight; 3) for Angelica sinensis, with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

J. Ethnopharmacol. 156, 309-315 (2014). HPTLC of (1) phyllanthin and (2) ellagic acid in the whole plant of Phyllanthus rheedei on silica gel with toluene - ethyl acetate - formic acid 6:2:1, detection at UV 366 nm. The hRF values of (1) and (2) were 47 and 27, respectively. Fractions rich in phyllanthin and ellagic acid showed anti-HBV and hepatoprotective activity.

lanceolata with an energy audit (thermal × microwave × acoustic):_x000D_ a case study of HPTLC determination with additional specificity using on-line/off-line_x000D_ coupling with DAD/NIR/ESI-MS. Phytochem. Anal. 25, 551-560 (2014). HPTLC of (1)taraxasterol, (2) taraxasterol acetate and (3) stigmasterol in P. lanceolata on silica gel with hexane - ethyl acetate 22:3, detection by spraying with anisaldehyde sulphuric acid reagent, followed by air drying for 40 min. Quantitative determination by absorbance measurement at 645 nm. The hRF values of (1) to (3) were 75, 33 and 19. Linearity was in the range of 1-5 μg/zone for (1) to (3). The intermediate intra-day and inter-day precisions were below 2.3 % (n=3). The LOD and LOQ were 530 and 1760 ng/zone for (1), 240 and 790 ng/zone for (2) and 110 and 370 ng/zone for (3), respectively. Recoveries were in the range of 98-105 % for (1), 101-131 % for (2) and 95-116 % for (3).