60th Indian Phamraceutical Congress PA-222 (2008). HPTLC of paracetamol, aceclofenac and rabeprazole on silica gel with ethyl acetate - methanol - glacial acetic acid 90:10:1. Quantitative determination by absorbance measurement at 275 nm. Linearity was in the range of 100-500 µg/mL, 20-100 µg/mL and 2-10 µg/mL for paracetamol, aceclofenac and rabeprazole respectively. Recovery was in the range of 98.9-100.1 % for all three compounds.

Abstract No. F-277, 61st IPC (2009). HPTLC of theobromine in different extracts of tea (Camelia sinensis) on silica gel with ethyl acetate - methanol 27:3. Quantitative determination by absorbance measurement at 274 nm. The maximum content of theobromine in tea samples was 2.3 %. Linearity was in the range of 3-15 µg/zone. The limit of detection and quantification was 30 and 140 ng/spot, respectively.

J. Planar Chromatogr. 22, 417-420 (2009). HPTLC of escitalopram oxalate and clonazepam on silica gel, prewashed with methanol, in a twin trough chamber saturated for 20 min at 25 °C with methanol - toluene - triethylamine 10:35:1. Quantitative determination by absorbance measurement at 253 nm.

var. cannabifolia (Sieb. et Zucc.) Hand. -Mazz. and Oleum Viticis Negundo by thin-layer chromatography) (Chinese). J. Chinese Trad. Med. & Pharm. (Shizhen Guoyi Guoyao) 20 (11), 2799-2800 (2009). TLC of TCM drug extracts on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 100:3. Detection by spraying with a solution of 5 % vanillin – 10 % sulfuric acid in ethanol 1:10 followed by heating at 105 °C until coloration, evaluation in visible and UV light.

Ind. J. Pharma. Sci. 8(4), 211-212 (2009). TLC of clopidogrel bisulphate on silica gel with carbon tetrachloride - ethyl acetate - ammonia 50:3:2. Quantitative determination by absorbance measurement at 230 nm. Linearity was between 300 and 1500 ng. In comparison with the labeled claim the amount of clopidogrel in tablets was 99.2 %. The recovery was 99.2 % (via peak area).

Abstract No. 9185, IHCB (2009). HPTLC of hydro alcoholic extracts of Berberis aristata on silica gel with benzene - ethyl acetate - diethyl amine 6:3:1. Detection by spraying with AlCl3 reagent (for estimation of flavonoids) or with Folin Ciocalteu reagent (for total phenolic content). The fingerprint profile was optimized using two different mobile phases: n-butanol - acetic acid - water 14:1:5 and n-propanol - formic acid - water 90:1:9. The extract showed 6 different spots and was found to contain 13.5 % w/w of berberin.

J. Pharma. Research 8(4), 187-191 (2009). HPTLC of abacavir sulphate and lamivudine on silica gel with methanol - acetone - n-butyl acetate 1:1:2. Quantitative determination by absorbance measurement at 284 nm. The hRf value of abacavir sulphate was 58 and of lamivudine 35. Linearity of abacavir sulphate and lamivudine was in the range of 240-1200 ng/spot and 120-600 ng/spot, respectively. The limit of detection and quantification of abacavir sulphate was 0.7 and 2 ng/spot, respectively, and of lamivudine 1 and 3 ng/spot, respectively.

Abstract No. F-270, 61st IPC (2009). HPTLC of fenoverine on silica gel with methanol - butyl acetate 1:4. The hRf value of fenoverine was 67. The linearity was in the range of 50 to 500 ng/band with a correlation coefficient of 0.9976. The limit of detection and quantification was 30 ng/band and 100 ng/band, respectively. The recovery was 100.1 %.