Biomed. Chromatogr. 19 (6), 474-478 (2005). HPTLC of enantiomers of verapamil on silica gel impregnated with vancomycin, a macrocyclic antibiotic, with acetonitrile - methanol – water 6:1:1. Detection by exposure to iodine vapors.

J. Planar Chromatogr. 20, 107-115(2007). TLC of atorvastatin, cerivastatin, fluvastatin, lovastatin, and simvastatin on silica gel, diol and cyano layers in horizontal chambers with binary mobile phases contaning hexane and a polar modifier in different proportions. Optimum normal-phase system was diol phase with hexane - tetrahydrofuran 3:2. Visualization under UV light at 254 nm. Quantitation by densitometry.

59th Indian Pharmaceutical Congress C-66, 240, (2007). Evaluation of Arjunakhseerpak and Arjunaristha, two ayurvedic formulations used for cardiovascular system, which contain Terminalia arjuna as the main ingredient. Simultaneous HPTLC of arjungenin and arjunolic acid from Terminalia arjuna on silica gel with toluene - ethyl acetate - acetic acid 10:10:1. Detection by spraying with 10 % sulphuric acid followed by heating. Densitometric evaluation at 366 nm. The UV spectra of the sandards arjungenin and arjunolic acid was found to be similar to that of arjungenin and arjunolic acid in the formulation. The method was found suitable for standardization of arjuna formulations.

Indian Drugs 44(10), 751 (2007). TLC and HPTLC of methanolic extracts of Eclipta alba, Launaea nudicaulis and Tridax procumbens, on silica gel with toluene - ethyl acetate - methanol 14:5:1. Evaluation under 254 and 366 nm. Detection by spraying with anisaldehyde sulfuric acid followed by evaluation at 525 nm.

Part II. Connection with RP18 and silica plates. J. Chromatogr. Sci. 46 (4), 291-297 (2008). Optimization of the one-dimensional TLC of alkaloid standards on cyano phase, RP-18W, and silica gel with various eluents containing silanol blockers (besides diluent and modifier), such as diethyl amine or ammonia. Separation of alkaloid mixtures with the most selective system (e.g. methanol - water 4:1 in the first direction and methanol - acetone - diisopropylether - diethylamine 15:15:69:1 in the second direction) with an adsorbent gradient method. Alkaloids or plant extracts of Chelidonium majus, Fumaria officinalis, or Glaucium flavum were chromatographed in the first system, the plates were connected with the plate pre-coated with various adsorbents, and partly separated fractions were transferred to the second layer and developed in a second system. Cyano - silica - RP-18W and cyano - silica - silica were used as the connected layers. The alkaloids were identified based on the Rf values of standards, and by comparison of UV spectra obtained by densitometry with a diode array detector.

Pharmazie 63, 102-106 (2008). TLC of benzyl 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside 1’,4-lactone, 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside 1’,4-lactone, and 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside-1-alpha-(diphenyl phosphate) 1’,4-lactone on silica gel with ethyl acetate - acetone 2:1. TLC of triethylammonium muramyl phosphate on silica gel with isopropanol - water - 25 % ammonia 6:1:3. Evaluation under UV light and by heating the plate at 250 °C.

Pharmazie 63, 580-584 (2008). TLC of 5-{4-[2-(5-ethyl-6-{4-[5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]benzyl}-1,3-thiazolidine-2,4-dione (pioglitazone), 5-{4-[2-(5-ethyl-4-{4-[2-[5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]benzyl}-1,3-thiazolidine-2,4-dione, and 5-{6,4’-bis-[2-(5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]biphenyl-3-ylmethyl}-1,3-thiazolidine-2,4-dione on silica gel with chloroform - methanol - ammonia 60:30:1. Detection under UV 254 nm.

J. Liq. Chromatogr. Relat. Technol. 31, 1857-1870 (2008). HPTLC of phospholipids (phosphatidic acid, phosphatidylcholin, phosphatidylethanolamine, phosphatidylinositiol, lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylinositol) on silica gel with chloroform - methanol - water - 25 % ammonia 60:34:4:2 in a twin-trough chamber saturated for 20 min. Plates were conditioned to 47 % relative humidity. Detection by dipping for 6 s in modified copper sulfate reagent (20 g copper (II) sulfate pentahydrate were dissolved in 200 mL of cooled methanol, then under cooling 8 mL of sulfuric acid 98 % and 8 mL of ortho-phosphoric acid 85 % were added) followed by drying in cold air for 30 s and heating at 140 °C for 30 min. Quantitative determination by absorbance measurement at 360 nm, 420 or 720 nm, or by video densitometry. Investigation of several parameters of the chromatographic process, including chamber saturation, derivatization, plate activity, and batch to batch consistency of the plates. For reproducible results, the employed methodology must be strictly standardized.