J. Ethnopharmacol. 174, 178-186 (2015). HPTLC of magnolol (1) and honokiol (2) in the cortex of Magnolia officinalis and aristolochic acid I (3) and II (4) in the radix of Aristolochia baetica on silica gel with methanol - ethyl acetate - toluene 1:2:30 for (1) and formic acid - water - ethyl acetate - toluene 1:1:10:20 for (2). Detection of (1) and (2) by spraying with vanillin reagent, followed by heating at 110 °C for 5 min. Detection of (3) and (4) by spraying with stannous chloride 100 g/L in diluted hydrochloric acid, followed by heating at 100 °C for 1 min. Identification under UV light at 365 nm for (3) and (4). The hRF values for (1) to (4) were 40, 50, 46 and 54, respectively.

J. Ethnopharmacol. 175, 324-334 (2015). HPTLC of betaine in Achyranthes aspera root extract on silica gel with methanol - water 9:1. Detection by spraying with Dragendorff's reagent followed by 10 % ethanolic sulfuric acid and drying at 110 °C for 5 min. Quantitative determination by absorbance measurement at 520 nm. The hRF value for betaine was 36. Linearity was in the range of 1-5 μg/zone. LOD and LOQ were 0.10 and 0.13 μg/zone. The intermediate precision was below 2 % (n=3).

J. Planar Chromatogr. 28, 354-361 (2015). TLC of rosuvastatin (1) and fenofibrate (2), rosuvastatin (1) and ezetimibe (3) and rosuvastatin (1) and aspirin (4) (in three different pharmaceutical combination products) on silica gel with toluene - acetone - acetic acid 30:10:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (4) were 19, 83, 43 and 53, respectively. Linearity was in the range of 500-4000 ng/zone for all four drugs. LOD and LOQ were 77 and 234 ng/zone for (1), 38 and 116 ng/zone for (2), 108 and 327 ng/zone for (3) and 144 and 438 ng/zone for (4), respectively. The intermediate precision was below 1 % (n=3). Average recovery ranged between 98.9 and 99.4 % for (1) to (4).

J. Planar Chromatogr. 29, 165-175 (2016). HPTLC of 15 steroid and phenanthrene derivatives on silica gel, RP-18 and CN phase with five binary mobile phases (acetonitrile – water, acetonitrile – dimethyl sulfoxide, acetonitrile – methanol, acetone – petroleum ether, and acetone – water). Quantitative structure‒retention relationships analysis showed the chromatographic behavior of the tested compounds.

J. AOAC Int. 99, 374-379 (2016). HPTLC of glabridin in the roots of Glycyrrhiza glabra on silica gel with hexane – ethyl acetate – chloroform 5:4:3. Quantitative determination by absorbance measurement at 285 nm. The hRF value of glabridin was 48. Linearity was in the range of 50-500 ng/zone. The LOD and LOQ were 10 ng/zone and 30 ng/zone, respectively. Intermediate precisions were below 2 %. Recovery was between 100.6 and 102.0 %. Compared to a validated HPLC method there was no statistically significant difference in the mean values.

CBS 115, 11-12 (2015). HPTLC of lysergic acid amides (ergot alkaloides) on silica gel with dichloromethane – methanol (or isopropanol) – triethylamine 9:1:0.002 with chamber saturation for 20 min to a migration distance of 70 mm. Evaluation under UV 254 nm and 366 nm. Densitometric evaluation by absorbance measurement at 254 nm. Target zones were directly eluted with methanol (with 1 % formic acid) to an ESI-MS and detected in positive ionization mode._x000D_

J. Liq. Chromatogr. Relat. Technol. 39, 271-276 (2016). HPTLC of ziprasidone and its impurities on silica gel with toluene – methanol – glacial acetic acid 15:1:1. Quantitative determination by absorbance measurement at 250 and 320 nm. The hRF value was 42 for ziprasidone and 19, 28, 31, 58 and 70 for its impurities. LOQ of impurities was 25 ng/zone. Recovery was between 94.9 and 106.7 %. See also J. Planar Chromatogr. 29, 239-246 (2016).

J. of Chromatogr. A 1415, 155-160 (2015). Presentation of a technique for direct analysis of raw samples by TLC coupled with electrospray ionization mass spectrometry (ESI-MS) instead of conventional MS analysis, which for raw samples commonly requires time-consuming and laborious sample pretreatment and separation using HPLC or GC. The analytes of interest could be extracted, ionized and detected by ESI-MS with much reduced matrix interference because the interfering compounds were retained by the sorbent material of the TLC plate. Demonstration by applying in direct analysis of samples containing common interfering compounds, e.g. salts and detergents. Rapid detection and quantification of target analytes in raw samples showed that the TLC-ESI-MS method was simple, rapid, efficient and could be effectively applied in offline and online separation and detection of different components in raw samples, e.g. plant extracts.