Pharmazie 63, 102-106 (2008). TLC of benzyl 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside 1’,4-lactone, 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside 1’,4-lactone, and 2-acetamido-6-O-acetyl-3-O-[(R)-1-carboxyethyl]-2-deoxy-alpha-D-glucopyranoside-1-alpha-(diphenyl phosphate) 1’,4-lactone on silica gel with ethyl acetate - acetone 2:1. TLC of triethylammonium muramyl phosphate on silica gel with isopropanol - water - 25 % ammonia 6:1:3. Evaluation under UV light and by heating the plate at 250 °C.

Pharmazie 63, 580-584 (2008). TLC of 5-{4-[2-(5-ethyl-6-{4-[5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]benzyl}-1,3-thiazolidine-2,4-dione (pioglitazone), 5-{4-[2-(5-ethyl-4-{4-[2-[5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]benzyl}-1,3-thiazolidine-2,4-dione, and 5-{6,4’-bis-[2-(5-ethylpyridin-2-yl)ethoxy]fenyl}pyrid-2-yl)-ethoxy]biphenyl-3-ylmethyl}-1,3-thiazolidine-2,4-dione on silica gel with chloroform - methanol - ammonia 60:30:1. Detection under UV 254 nm.

J. Liq. Chromatogr. Relat. Technol. 31, 1857-1870 (2008). HPTLC of phospholipids (phosphatidic acid, phosphatidylcholin, phosphatidylethanolamine, phosphatidylinositiol, lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylinositol) on silica gel with chloroform - methanol - water - 25 % ammonia 60:34:4:2 in a twin-trough chamber saturated for 20 min. Plates were conditioned to 47 % relative humidity. Detection by dipping for 6 s in modified copper sulfate reagent (20 g copper (II) sulfate pentahydrate were dissolved in 200 mL of cooled methanol, then under cooling 8 mL of sulfuric acid 98 % and 8 mL of ortho-phosphoric acid 85 % were added) followed by drying in cold air for 30 s and heating at 140 °C for 30 min. Quantitative determination by absorbance measurement at 360 nm, 420 or 720 nm, or by video densitometry. Investigation of several parameters of the chromatographic process, including chamber saturation, derivatization, plate activity, and batch to batch consistency of the plates. For reproducible results, the employed methodology must be strictly standardized.

CBS 101, 2-4 (2008). HPTLC of amino acids (from hydrolysis of peptides) on silica gel, Diol phase, and cellulose with either 2-butanol - acetic acid - pyridine - water 15:3:10:12 or 2-butanol - 25 % ammonia - pyridine - water 39:10:34:26 in a twin-trough chamber or horizontal chamber. Detection by dipping in ninhydrin solution (0.5 % in 2-propanol) followed by heating at 110 °C for 5 min. Better results are achieved by adding ninhydrin directly to the mobile phase at a 0.5 %-level. Quantitative determination by absorbance measurement at 440 nm. Selectivity was better on the cellulose and silica gel plate. Selection of the chromatographic system depended on which amino acids had to be separated and no general recommendation could be given. HPTLC analysis of a hydrolyzed peptide sample (containing Phe, Trp, D-Bal, Apc and Inp) on cellulose with the acidic mobile phase. All five amino acids were quantified between 70 and 130 % of the theoretical value for non-stable amino acids (degradation 5 to 30 %).

60th Indian Pharmaceutical Congress PA-205, (2008). HPTLC of diacerein on silica gel with ethyl acetate - n-hexane - acetic acid 120:19:1. The hRf value was 32. Quantitative determination by absorbance measurement at 258 nm. The method was linear in the range of 50-250 ng/spot. The recovery was 96-103 %.

J. Liq. Chromatogr. Relat. Technol. 30, 2337-2344 (2007). TLC of pralidoxime and obidoxime on silica gel impregnated by an 18 hours continous development with 10 % paraffin oil in n-hexane in an unsaturated chamber. Lipophilicity was determined on paraffin coated TLC plates using 30 %, 40 %, 50 %, 60 %, 70 %, and 80 % aqueous methanol, each mixture containing 1 % ammonium hydroxide). Hydrophilicity was determined by using plain silica gel with 70 %, 80 %, and 90 % aqueous methanol and 100 % methanol, each mixture containing 1 % ammonium hydroxide. Detection under white light and under UV 254 nm.

Asian J. Chem. 19(6), 4286 - 4290 (2008). HPTLC of rofecoxib and tizanidine hydrochloride on silica gel with acetone - methanol 1:1. The method was linear in the range of 2200-3300 ng/spot and 180-260 ng/spot for rofecoxib and tizanidine respectively. The recovery was between 99.7 and 102.6 % for both compounds. The method was useful for the simultaneous estimation of the drug content in pure and tablet dosage form.

Asian J. Chem. 18(2), 1078-1084 (2006). TLC of nimesulide and diclofenac sodium on silica gel with n-hexane - ethyl acetate - chloroform - acetic acid 16:2:3:1. Quantitative determination by absorbance measurement at 256 nm. The hRf value of nimesulide was 3 and of diclofenac sodium 57. Linearity was between 1.0 and 3.2 µg/zone for nimesulide and 0.5 and 1.6 µg/zone for diclofenac sodium. The recoveries (by standard addition method) were in the range of 99.1 and 101.0 for both drugs. The proposed method is precise and accurate and can be used for routine analysis of nimesulide and diclofenac sodium capsule formulation.